Free Amino Acid Composition in Hemolymph and Muscle of the Ghost Crab, Ocypode platytarsis

Sankar, R; Yogamoorthi, A.

doi:10.3923/pjbs.2012.490.495

Cited by 7 publications

(10 citation statements)

References 18 publications

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“…These in partially agreement with the obtained results whereas it stated the highly relevance of GLU (non-essential AA). But we can't generalize that where AA differ in its composition according to studded insect tissue (Sankar & Yogamoorthi, 2012); insect and the insect stage (Haag & Sullivan, 1984 and our un published data on PBW); host plant (PAL et al, 1973) and method used for detecting AA (Kleiner & Peacock, 1971).…”

Section: Resultsmentioning

confidence: 90%

“…According to the average of AA calculated in Table (2) it could be said that PBW had the highest average of AA (1.89, 1.32 & 2.41 for essential, semi-essential & nonessential AA, respectively), followed by APL (1.78, 1.22 & 2.34, respectively), then CLW (1.66, 1.16 & 2.17, respectively). Amino acids have long been recognized to differ in its composition according to studded insect (Haag &Sullivan, 1984 andSankar &Yogamoorthi, 2012), so it could be used as a taxonomic tool (Schaefer & Wallace, 1967). In addition, it also differed according to the studied insect stage ( (Haag & Sullivan, 1984 and our unpublished data on different stages of PBW); host plant (PAL et al, 1973) and detecting method (Kleiner & Peacock, 1971).…”

Section: Resultsmentioning

confidence: 99%

“…These percentages for essential AA were 34, 33 & 33% for PBW, CLW and APL, respectively, and for semi-essential AA were 23, 23 & 23% for PBW, CLW and APL, respectively. Sankar & Yogamoorthi (2012) mentioned that the non-essential amino acids play a more important part than the essential ones in the cellular osmotic pressure regulation.…”

Section: Resultsmentioning

confidence: 99%

“…Understanding of their chemical composition of amino acids (AA) has potential practical significance. Amino acids are not only the building blocks of proteins but are also key regulators of various pathological and physiological processes (Sankar & Yogamoorthi, 2012); including immune responses (Yoneda et al, 2009), isosmotic intracellular regulation (Weber & van Marrewijk, 1972), osmoregulation (Van Waarde, 1988) and neurotransmission (Anaya & D'Aniello, 2006). Also, it used as source of energy producing compounds (Van Marrewijk & Ravestein et al, 1974), as a taxonomic tool (Schaefer & Wallace, 1967), and for the determination of nutritional requirements (Kleiner & Peacock, 1971).…”

Section: Introductionmentioning

confidence: 99%

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Comparative Studies on the Free Amino Acid Composition in the Total Body Homogenate of some Lepidopterous and Neuropterous Insects دراسة لمقارنة محتوى الأحماض الأمینیة لبعض حشرات رتبتی حرشفیة وشبکیة الأجنحة

الشناوی¹,

أحمد²,

الشرقاوى³

2019

Journal of Plant Protection and Pathology

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Three different insect species belonging to three families [i.e. P. gossypiella (PBW), S. littoralis (CLW) and C. carnea (APL)] were studied for their amino acid composition. Seventeen free amino acids (FAA) figured out in the total body homogenate of the tested insects. The essential AA were threonine (THR), valine (VAL), methionine (MET), isoleucine (ILE), leucine (LEU), phenylalanine (PHE), lysine (LYS). Semi-essential AA were histidine (HIS), arginine (ARG), cysteine (CYS), and Non-essential were aspartic acid (ASP), serine (SER), glutamic acid (GLU), glycine (GLY), alanine (ALA), tyrosine (TYR), and proline (PRO). Generally, results showed that non-essential AA were the most predominant fraction of FAA, following by essential then semi-essential AA, and the PBW had the highest average of AA (1.89, 1.32 & 2.41 for essential, semi-essential & non-essential AA, respectively), followed by APL (1.78, 1.22 & 2.34, respectively), then CLW (1.66, 1.16 & 2.17, respectively). In details, from the results it could be grouped AA in 3 groups as follows: 1-A high AA concentration (more than 3 mg/100mg). 2-A moderate AA concentration (more than 2 mg/100mg). 3-A low AA concentration (less than 2 mg/100mg).The results showed that the PBW characterized by a high AA concentration of ASP (3.19 mg/100mg) and GLU (4.24 mg/100mg), while CLW and APL had only a high AA concentration of GLU (3.98 & 5.24 mg/100mg, respectively), and a moderate concentration of ASP (2.46 & 2.90 mg/100mg, respectively). In addition, GLU had a highest concentration in APL. The obtained results indicated that glutamic acid and aspartic acid (non-essential AA) were the most predominant FAA. For moderate AA, PBW characterized by an amino acids VAL and TYR (2.28 mg/100mg; 17.26% & 2.53 mg/100mg; 13.88%, respectively). In contrast, CLW and APL had a low VAL and TYR concentration (1.73 & 1.78 mg/100mg; 14.91 & 14.25% & 1.63 & 1.87 mg/100mg; 9.96 & 10.61% for VAL & TYR, respectively). Also, ALA had a moderate concentration in PBW (2.28 mg/100mg) and CLW (2.50 mg/100mg), while had a low concentration in APL (1.83 mg/100mg). In addition, CYS had a least concentration of AA (0.55, 0.59 and 0.57 mg/100mg for PBW, CLW and APL, respectively.

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Section: Resultsmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparative Studies on the Free Amino Acid Composition in the Total Body Homogenate of some Lepidopterous and Neuropterous Insects دراسة لمقارنة محتوى الأحماض الأمینیة لبعض حشرات رتبتی حرشفیة وشبکیة الأجنحة

الشناوی¹,

أحمد²,

الشرقاوى³

2019

Journal of Plant Protection and Pathology

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“…Thirteen amino acids made up of 8 EAA and 5 NEAA were isolated from M. vollenhovenii and Tympanotonus fuscatus from Benin River, Nigeria [8], while Abdel-Salam [9] reported 9 EAA and 7 NEAA in important crustaceans from Egyptian and Saudi Arabia coasts. For crabs, Sudhakar et al [10] and Sankar and Yogamoorthi [11] reported 7 EAA, 7 NEAA and 7 EAA, 5 NEAA in Ocypode platytarsis and Portunis sanguinolentus respectively. The nutritive value of any animal is determined by the presence of EAAs [10].…”

Section: Resultsmentioning

confidence: 99%

Amino Acid Composition of Macrobrachium vollenhovenii and Callinectes amnicola from Badagry Creek, Lagos, Nigeria

Jimoh¹,

Clarke²,

II³

2019

SARJAF

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Amino acid composition of the body parts of Macrobrachium vollenhovenii and Callinectes amnicola collected from Badagry creek were assessed and determined. Fifteen and twelve samples of M. vollenhovenii and C. amnicola respectively were used in the study. The weight of the prawns ranged from 4.09g-17.50g while the crab ranged from 31.00g-119.82g. The prawns were separated into three (3) different parts which are the whole prawn, flesh and exoskeleton while the crabs were limited to the whole crab and the flesh. Chemical analysis was carried out following the modified method AOAC method 982.30, while the data obtained were analyzed using SPSS version 20.0. Eighteen amino acids were reported made up of ten (10) essential amino acids (EAA) and eight non-essential amino acids (NEAA). The EAA were arginine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan and valine, while the NEAA were glycine, alanine, proline, aspartate, glutamate, tyrosine and cystine. The ratio of EAA: NEAA in M. vollenhovenii and C. amnicola was 1.25. There were significant differences (p=0.05) among the amino acids from the various parts analysed. Glutamate recorded the highest concentration while cystine recorded the lowest concentration in both crustaceans. Thus, these crustaceans probably play important roles in learning and sensitivity due to the concentration of glutamate which is a powerful excitatory neurotransmitter that is released by nerve cells in the brain. On the basis of nutrition and higher concentration of amino acids, M. vollenhoveni and C. amnicola are better sources of protein and can serves as substitutes for other proteinous food.Food and tissue proteins contain 20 different amino acids including essential and non-essential amino acids of nutritional importance. Shellfishes contain varying levels of high quality protein rich in all the valuable dietary essential amino acids. Amino acids are mainly obtained from proteins in diet therefore a sufficient quantity of dietary protein is required for growth, survival, development, reproduction and maintaining good health throughout life [3,4].Since amino acids have been reported to have a lot of biological importance, and prawns/crabs are regarded as good sources of proteins, this study was carried out to determine the amino acid composition of the African river prawn, Macrobrachium vollenhovenii and the crab, Callinectes amnicola from Badagry Creek, Lagos, Southwest Nigeria.

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External Influences on Invertebrate Brain Histamine and Related Compounds via an Automated Derivatization Method for Capillary Electrophoresis

Parrot

Pavón-Vergés

Perrot‐Minnot

et al. 2017

ACS Chem. Neurosci.

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Histamine has been shown to modulate visual system and photic behavior in arthropods. However, few methods are available for the direct quantification of histamine and its precursor and metabolites in arthropod brain. In this work, a method for the separation of histamine, its precursor histidine, and its metabolite N-methyl-histamine from brain extracts of a freshwater crustacean has been developed using capillary electrophoresis with laser-induced fluorescence detection. Molecules were tagged on their primary amine function with naphthalene-2,3-dicarboxaldehyde, but derivatized histamine and N-methyl-histamine exhibited poor stability in contrast to derivatized histidine. To overcome this limitation, an automated derivatization performed within the capillary electrophoresis instrument was optimized and quantitatively validated. The limits of detection were 50, 30, and 60 nmol/L for histidine, histamine, and N-methyl-histamine, respectively. This study reports, for the first time, the amounts of histamine and its related compounds in brain extracts from populations of the freshwater amphipod Gammarus fossarum, and shows that these amounts vary mainly according to population and season, but are not affected by an experimental electrical shock.

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Free Amino Acid Composition in Hemolymph and Muscle of the Ghost Crab, Ocypode platytarsis

Cited by 7 publications

References 18 publications

Comparative Studies on the Free Amino Acid Composition in the Total Body Homogenate of some Lepidopterous and Neuropterous Insects دراسة لمقارنة محتوى الأحماض الأمینیة لبعض حشرات رتبتی حرشفیة وشبکیة الأجنحة

Comparative Studies on the Free Amino Acid Composition in the Total Body Homogenate of some Lepidopterous and Neuropterous Insects دراسة لمقارنة محتوى الأحماض الأمینیة لبعض حشرات رتبتی حرشفیة وشبکیة الأجنحة

Amino Acid Composition of Macrobrachium vollenhovenii and Callinectes amnicola from Badagry Creek, Lagos, Nigeria

External Influences on Invertebrate Brain Histamine and Related Compounds via an Automated Derivatization Method for Capillary Electrophoresis

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