Freezability of rat epididymal sperm induced by raffinose in modified Krebs–Ringer bicarbonate (mKRB) based extender solution

Yamashiro, Hideaki; Han, Young Joon; Sugawara, Atsushi; Tomioka, Ikuo; Hoshino, Yumi; Sato, Eimei

doi:10.1016/j.cryobiol.2007.08.011

Cited by 28 publications

(36 citation statements)

References 45 publications

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“…T h e i n t e g r i t y o f t h e s p e r m a c r o s o m e w a s evaluated using fluorescein isothiocyanate-labelled peanut (A. Hypogaea) agglutinin (FITC-PNA) staining [8,[24][25][26][27]. Ten µL of diluted semen with 140 µL phosphate buffered saline (PBS) was mixed with 10 µL of EthD-1 and incubated at 37ºC for 15 min.…”

Section: Assessment Of Acrosomal Integritymentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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Section: Assessment Of Acrosomal Integritymentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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show abstract

“…Assessment of acrosomal integrity.The integrity of the sperm acrosome was evaluated with fluorescein isothiocyanate-labelled peanut (Arachis hypogaea) agglutinin (FITC-PNA) staining [15,30,34,52]. Ten microliters of diluted semen with 140 µl phosphate buffered saline (PBS) was mixed 10 µl of ethidiumhomodimer-1 and incubated at 37°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Kaeoket

Donto

Nualnoy

et al. 2012

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of this study was to determine the effect of gamma-oryzanol-enriched -rice bran oil on the quality of cryopreserved boar semen. Ten boars provided semen of proven motility and morphology for this study. The semen was divided into three portions in which lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with 2 types of rice bran oils, at a gamma-oryzanol concentration of 0 mg/ml of lactose egg yolk (LEY) freezing extender (group A, control), 0.1 mg/ml(0.16 mMol) of freezing extender (group B) and 0.1 mg/ml of freezing extender (group C). Semen suspensions were loaded in medium straws (0.5 ml) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability and acrosomal integrity. There was a significantly higher percentage of progressive motility (34 versus 47.0 and 48.5, P<0.001), viability (35.5 versus 48.1 and 50.1, P<0.001) and acrosomal integrity (39.8 versus 50.8 and 54.9, P<0.001) in the gamma-oryzanolenriched rice bran oil-supplemented groups (groups B, C) than in the control group (group C), respectively. In conclusion, addition of gamma-oryzanol-enriched rice bran oil to LEY freezing extender is appropriated for improving the quality of frozen-thawed boar semen. Cryopreservation of boar spermatozoa offers an effective way to maintain their valuable genetic material and transport it between countries, thereby minimizing boar transportation. It has been demonstrated that certain processes in cryopreservation including elimination of seminal plasma by centrifugation and reduction in the temperature result in cold shock, owing to a decrease in quantity of antioxidants and an increase in reactive oxygen species (ROS) contents in the semen [6,8,43]. The ROS can lead to oxidative stress and lipid peroxidation of the sperm plasma membrane, which have detrimental effects on spermatozoa during cryopreservation, for instance, reduction in progressive motility, acrosomal integrity, sperm-oocyte fusion and fertilizing potential [1-3, 17, 41, 53].During the past decade, several studies have focused on supplementation of freezing extender for frozen boar semen with a variety of antioxidants (e.g., vitamin E, vitamin C, L-cysteine, glutathione, taurine, pyruvate, SOD, catalase) in an attempt to minimize the detrimental effects of ROS, which occur during the freezing process [7,10,16,23,26,38,39].Gamma-oryzanol, a phytosteryl ferulate mixture extracted from rice bran oil, has received a great deal of attention, because of its various significant health-promoting functions such as antioxidant activity, inhibition of lipoperoxidation by its scavenging activity [4], reduction in LDL cholesterol and induction of HDL cholesterol [40,41,48] and inhibition of platelet aggregation [13]. In humans, many studies have been reported on its potential implications as a UV-A filter in sunscreen cosmetics [14] and as a treatment for type 2 diabetes mellitus [12] and allergic reactions [3...

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“…We first studied to develop an ideal freezing extender and method for rat sperm cryopreservation (Yamashiro et al, 2007). Experiments were conducted to study its postthaw characteristics when freezing with raffinose-free buffer or various concentrations of raffinose and egg yolk dissolved in distilled and deionised water, PBS, or mKRB based extender.…”

Section: Freezability Of Rat Sperm Induced By Raffinose In Modified Kmentioning

confidence: 99%

Cryopreservation of Rat Sperm

Yamashiro¹,

Sato²

2012

Current Frontiers in Cryopreservation

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Freezability of rat epididymal sperm induced by raffinose in modified Krebs–Ringer bicarbonate (mKRB) based extender solution

Cited by 28 publications

References 45 publications

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Cryopreservation of Rat Sperm

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