Frequency and potential application of HLA antibodies from pregnant women in Mumbai

Shankarkumar, U.; Gupte, Snehalata C.; Pednkar, S. V.; Ghosh, Kanjaksha; Mohanty, Dipika

doi:10.1007/bf02709171

Cited by 13 publications

(9 citation statements)

References 14 publications

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“…HLA‐A, ‐B, and ‐C locus antigens were identified by the National Institute of Health (NIH) two‐stage microlymphocytotoxicity assay (Terasaki & McClelland, 1964). A total of 190 antisera, indigenous (Shankarkumar et al ., 1998) as well as commercial (Biotest; BAG, Dreieich, Germany), were used to define 17 specificities for the HLA‐A locus antigens, 29 for the HLA‐B locus antigens, and eight for the HLA‐C locus antigens. The typing tray included a minimum of three antisera for each supertypic specificity.…”

Section: Methodsmentioning

confidence: 99%

Molecular diversity of HLA‐Cw alleles in the Maratha community of Mumbai, Maharashtra, western India

Shankarkumar

Ghosh

Mohanty

2005

Int J Immunogenetics

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Recent advances suggest a significant role for the HLA-C locus as a target of alloreactions after bone marrow transplantation. The biological importance of products of the HLA-C locus, both as transplant antigens and as ligands for natural killer (NK) cells, is well established. A total of 10 different serologically defined HLA-Cw antigen specificities (Cw1-Cw10) are encoded by the C locus; however, there are now 151 different alleles that can be identified by molecular methods. Serological definition of Cw alleles therefore includes 20-50% blanks, which cannot be detected by the available antisera. We used the molecular method of polymerase chain reaction (PCR)-based sequence-specific amplification and probe hybridization to define Cw alleles in 91 individuals from the Maratha community, and compared the data with data for 92 serologically typed Maratha individuals from India. We identified Cw*12, Cw*14, Cw*15, Cw*16 and Cw*18, along with the serologically identified Cw*01, Cw*02, Cw*03, Cw*04, Cw*06 and Cw*07 alleles. The HLA-Cw blank allele frequency in the Maratha was reduced from 0.5706 to 0.00. Furthermore, by using a molecular technique, it was possible to identify novel allele subtypes, such as Cw*0104, Cw*0203 and Cw*0707, and a high frequency of Cw* 1801 in the Maratha community compared with other Indian and world populations. Our results will have clinical implications in related and unrelated HLA-matched bone marrow transplantation in India.

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Section: Methodsmentioning

confidence: 99%

Molecular diversity of HLA‐Cw alleles in the Maratha community of Mumbai, Maharashtra, western India

Shankarkumar

Ghosh

Mohanty

2005

Int J Immunogenetics

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“…A total of 190 antiserum were used for defining 17 specificities for HLA A locus, 29 for HLA B locus, 8 for HLA C locus and 10 for HLA DR locus antigens. The antisera were commercial (Biotest, Germany; Behring, Germany; Pelfreez, USA) as well as indigenous (Shankarkumar et al 1998) in origin. The typing tray included a minimum of three antisera for each supertypic specificity.…”

Section: Methodsmentioning

confidence: 99%

HLA Antigens in Nadars a Native Dravidian Caste Group of Tamil Nadu, South India

Sridharan

Shankarkumar

2004

International Journal of Human Genetics

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One hundred and thirty seven unrelated Nadar individuals residing in Tamil Nadu, (South India) were studied for HLA A, B, C and DR locus antigen profiles. The allele frequencies of HLA A9, B5, Cw4, Cw7, DR2 and DR6 were increased while frequencies of HLA A10, B8, B16, B46, B78, Cw5, Cw9 and DR9 were decreased in the Nadars. The gene frequencies of HLA A1, A3, A28, B5, B17, B37, Cw4, Cw5, DR1, DR6 and DR10 were increased while that of A2, A19, B16, B46, Cw3, DR5 and DR9 were decreased when compared with gene frequencies of other Asian populations reported. Two Locus haplotype analyses revealed that A11-B5, A1-B17, A3-B44, A24-B51, B5-Cw9, B44-Cw1, DR4-DQ2 and DR6-DQ1 were significant haplotypes among the positive linkage disequilibrium haplotypes. Where as A1-B5, B5-Cw1 and DR1-DQ2 were significant haplotypes among the negative linkage disequilibrium haplotypes. The haplotypes A3-B44, A24-B51, B44-Cw1 and DR6-DQ1 observed in Nadars were unique when compared to other Indian populations reported in literature. The observed antigen frequencies and linkage disequilibrium in Nadars suggest the influence of genetic drift caused by selection, geography and culture with a lesser degree of admixture. Further the study reveals that the Nadar population of India cannot be considered as a single panmictic population with reference to genetic characteristics, which may have a clinical relevance in unrelated donor selection for allogenic Bone marrow transplantation in India.

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“…A minimum of two to three antisera was used for each specificity. The antisera were commercial (Biotest; Pelfreez) and indigenous [13] in origin.…”

Section: Hla Serological Typingmentioning

confidence: 99%

Immunogenetic association in patients with antineutrophil cytoplasmic antibodies (ANCA) from Mumbai, Maharashtra, India

Shankarkumar

Ghosh

Pradhan

et al. 2005

Journal of Autoimmunity

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Frequency and potential application of HLA antibodies from pregnant women in Mumbai

Cited by 13 publications

References 14 publications

Molecular diversity of HLA‐Cw alleles in the Maratha community of Mumbai, Maharashtra, western India

Molecular diversity of HLA‐Cw alleles in the Maratha community of Mumbai, Maharashtra, western India

HLA Antigens in Nadars a Native Dravidian Caste Group of Tamil Nadu, South India

Immunogenetic association in patients with antineutrophil cytoplasmic antibodies (ANCA) from Mumbai, Maharashtra, India

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