Frequency of circulating iNKT cells among Iranian healthy adults

Fereidouni, Mohammad; Hosseini, Reza Farid; Azad, Farahzad Jabbari; Schenkel, Jason M.; Varasteh, Abdolreza; Mahmoudi, Mahmoud

doi:10.1002/cyto.b.20489

Cited by 11 publications

(15 citation statements)

References 38 publications

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“…Our results are in line with the results from two studies showing lower circulating NKT cell levels in male subjects [19,27]. However, no gender-related differences of NKT cell frequency were found in other previous studies [23][24][25], possibly because of NKT cell marker, sample size, sex ratio, subject age range, and inclusion or exclusion criteria differences. In the present study, a gender-related difference in circulating NKT cell levels was only found in elderly subjects.…”

Section: Discussionsupporting

confidence: 92%

“…This unusually high level in a healthy individual has been previously reported [29]. However, when this individual was excluded, the distribution of circulating NKT cell frequency found in the present study was similar to those found in previous studies [21,23,25].…”

Section: Discussionsupporting

confidence: 92%

“…Our findings regarding the high variability shown by circulating NKT cell levels in healthy populations are supported by previous reports of levels ranging from the limit of detection at 0.01% to 2% [21,23,25,26]. However, in the present study, the range was even wider (0.01-5.15%), which is probably due to the inclusion of a healthy male subject with a NKT cell proportion of >5% of total peripheral blood lymphocytes and >7% of total T cells.…”

Section: Discussionsupporting

confidence: 65%

“…Reductions in circulating NKT cell levels have been reported for several disorders, such as autoimmune diseases [13][14][15][16], chronic infections [17,18], and tumors [19,20]. Furthermore, several human studies have reported wide ranges of age-and/or sex-related variations in circulating NKT cell levels among healthy subjects [19,[21][22][23][24][25][26][27]. However, most studies have been performed in small Caucasian populations with narrow age ranges or unbalanced sex ratios.…”

Section: Introductionmentioning

confidence: 96%

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Age- and gender-related differences in circulating natural killer T cells and their subset levels in healthy Korean adults

et al. 2012

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 96%

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Age- and gender-related differences in circulating natural killer T cells and their subset levels in healthy Korean adults

et al. 2012

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“…Detection of iNKT cells using 6B11 and anti-CD3 mAbs tends to yield higher frequencies compared with detection using C15 and anti-Vβ11 mAbs (46, 47), which might be because not all Vα24i pairs with Vβ11. However, the analyses in those studies were not conducted in a pairwise manner.…”

Section: Discussionmentioning

confidence: 94%

Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs

Chamoto

Guo

Scally

et al. 2017

The Journal of Immunology

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The human invariant natural killer T (iNKT) TCR is largely composed of the invariant TCR Vα24-Jα18 chain and semi-variant TCR Vβ11 chains with variable CDR3β sequences. The direct role of CDR3β in antigen recognition has been extensively studied. Although it has been noted that CDR3β can interact with CDR3α, how this interaction might indirectly influence antigen recognition is not fully elucidated. We observed that the third position of Vβ11 CDR3 can encode an arginine (Arg) or serine (Ser) residue as a result of somatic rearrangement. Clonotypic analysis of the two iNKT TCR types with a single amino acid substitution revealed that the staining intensity by anti-Vα24 antibodies depends on whether Ser or Arg is encoded. When stained with an anti-Vα24-Jα18 antibody, human primary iNKT cells could be divided into Vα24 low- and high-intensity subsets, and Arg-encoding TCR Vβ11 chains were more frequently isolated from the Vα24 low-intensity compared with the Vα24 high-intensity subpopulation. The Arg/Ser substitution also influenced antigen recognition as determined by CD1d multimer staining and CD1d-restricted functional responses. Importantly, in silico modeling validated that this Ser to Arg mutation could alter the structure of not only the CDR3β but also the CDR3α loop. Collectively, these results indicate that the Arg/Ser encoded at the third CDR3β residue can effectively modulate the overall structure of and antigen recognition by human iNKT TCRs.

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Evaluation of cord blood immunoglobulin E and its association with maternal factors in a group of Iranian newborns

Fereidouni

Nami

Serki

et al. 2019

J of Cellular Biochemistry

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Allergic disorders are among the most common diseases around the world especially in children. Many factors contribute to the pathogenesis of atopic disorders, but early events during the pregnancy are very important. The aim of this study was to evaluate the level of cord blood immunoglobulin E (CB-IgE) and its association with maternal in a group of Iranian newborns. In a crosssectional study, 163 pregnant women randomly selected and information about pregnancy and atopy were taken by questionnaire. Blood samples of mothers and matched cord blood were collected and total serum IgE levels were measured by enzyme-linked immunosorbent assay (ELISA) method. To rolling out the possibility of contamination with maternal blood, total IgA was checked for all the cord blood samples. Sixteen percent of mothers had the history of atopic diseases and the mean IgE level was significantly higher in an atopic than nonatopic mothers (241 vs 102, P < 0.001). About 73.9% of cord blood samples, had high IgE level (>0.9 IU/mL). The level of cord blood IgE (CB-IgE) was not significantly different in male and female newborns (2.14 vs 2.15 IU/mL). There was no significant correlation between maternal factors such as age, pregnancy variables, allergens exposure, smoking, and maternal IgE with cord blood IgE.The results of this study showed that CB-IgE is high in a remarkable number of samples; independent of maternal or fetal factors. Further studies need to evaluate the reasons for the high level of IgE in cord blood in our area. K E Y W O R D S allergic predisposition, cord blood immunoglobulin E, Iranian newborns, maternal exposure, sensitization

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Frequency of circulating iNKT cells among Iranian healthy adults

Cited by 11 publications

References 38 publications

Age- and gender-related differences in circulating natural killer T cells and their subset levels in healthy Korean adults

Age- and gender-related differences in circulating natural killer T cells and their subset levels in healthy Korean adults

Key Residues at Third CDR3β Position Impact Structure and Antigen Recognition of Human Invariant NK TCRs

Evaluation of cord blood immunoglobulin E and its association with maternal factors in a group of Iranian newborns

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