Frequent Tn
            <i>2</i>
            Misannotation in the Genetic Background of
            <i>rmtB</i>

Martins, Willames M. B. S.; Gales, Ana Cristina

doi:10.1128/aac.00811-17

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…In China, rmtB is also the main prevalent genotype of 16S-RMTase and is often present in combination with armA [28]. Further genetic environment studies of rmtB in pMAS152 show that rmtB is located downstream of ∆Tn2 (tnpR-bla TEM-1B ), which is consistent with the classical genetic environment of rmtB [29]. In addition, we also found that the genetic environment nahP-groEL-IS91, located downstream of rmtB, is relatively conserved on plasmids in both P. aeruginosa and other Gram-negative bacilli, presenting the conserved genetic environment ∆Tn2-rmtB-nahP-groEL-IS91.…”

Section: Discussionsupporting

confidence: 58%

Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa

Mei,

Song,

Liu

et al. 2024

Microorganisms

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Bacterial antimicrobial resistance (AMR) poses a significant global public health challenge. The escalation of AMR is primarily attributed to the horizontal gene transfer (HGT) of antibiotic resistance genes (ARGs), often facilitated by plasmids. This underscores the critical need for a comprehensive understanding of the resistance mechanisms and transmission dynamics of these plasmids. In this study, we utilized in vitro drug sensitivity testing, conjugation transfer assays, and whole-genome sequencing to investigate the resistance mechanism of an extensively drug-resistant (XDR) Pseudomonas aeruginosa clinical isolate, MAS152. We specifically focused on analyzing the drug-resistant plasmid pMAS152 it harbors and its potential for widespread dissemination. Bioinformatics analysis revealed that MAS152 carries a distinct IncpP-2A plasmid, pMAS152, characterized by a 44.8 kb multidrug resistance (MDR) region. This region houses a 16S rRNA methyltransferase (16S-RMTase) gene, rmtB, conferring high-level resistance to aminoglycoside antibiotics. Notably, this region also contains an extended-spectrum β-Lactamase (ESBL) gene, blaPER-1, and an efflux pump operon, tmexCD-oprJ, which mediate resistance to β-Lactams and quinolone antibiotics, respectively. Such a combination of ARGs, unprecedented in reported plasmids, could significantly undermine the effectiveness of first-line antibiotics in treating P. aeruginosa infections. Investigation into the genetic environment of the MDR region suggests that Tn2 and IS91 elements may be instrumental in the horizontal transfer of rmtB. Additionally, a complex Class I integron with an ISCR1 structure, along with TnAs1, seems to facilitate the horizontal transfer of blaPER-1. The conjugation transfer assay, coupled with the annotation of conjugation-related genes and phylogenetic analysis, indicates that the plasmid pMAS152 functions as a conjugative plasmid, with other genus Pseudomonas species as potential hosts. Our findings provide vital insights into the resistance mechanisms and transmission potential of the XDR P. aeruginosa isolate MAS152, underlining the urgent need for novel strategies to combat the spread of AMR. This study highlights the complex interplay of genetic elements contributing to antibiotic resistance and underscores the importance of continuous surveillance of emerging ARGs in clinical isolates.

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Section: Discussionsupporting

confidence: 58%

Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa

Mei,

Song,

Liu

et al. 2024

Microorganisms

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“…The rmtB (aminoglycoside resistance)-carrying Tn2-rmtB element [15] and its variants [16] were widely found in resistance plasmids from Enterobacteriaceae species. Tn6377 was initially found in pKP96 from K. pneumoniae [17], and generated from insertion of the ISEcp1-bla CTX-M-65 -IS903D unit [18] at a site within the methyl-accepting chemotaxis gene mcp of the Tn3-family unit transposon Tn1722 [19].…”

Section: The Mdr Regions Of Phn7a8 Pct-kpc and P675920-1mentioning

confidence: 99%

WITHDRAWN: Structure genomics of two chimera plasmids p675920-1 and p675920-2 coexisting in a multi-drug resistant Klebsiella pneumoniae isolate

Feng¹,

Yin²,

Zhan³

et al. 2018

Oncotarget

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This study dealt with detailed genomic characterization of two multidrug resistant (MDR) plasmids p675920-1 and p675920-2 from a single clinical Klebsiella pneumoniae isolate 675920. p675920-1 was essentially a hybrid of the IncFII plasmid pHN7A8 and the IncR plasmid pKPC-LK30, and functioned as an IncFII plasmid with inactivation of the IncR replication gene. The backbone of p675920-2 was a hybrid of a novel replicon, three maintenance regions (22.0-, 2.7-, 2.6-kb in length, respectively) as found in pKPYL2, p10164-3 and pK1HV, respectively, and the entire 25.9-kb conjugal transfer region of pKPYL2. p675920-1 and p675920-2 carried a large number of resistance genes, which contributed to resistance to at least seven classes of antibiotics (β-lactams, quinolones, aminoglycosides, fosfomycins, sulphonamides, trimethoprims, and tetracyclines) and one kind of heavy mental (mercury). All of these resistance genes are associated with mobile elements such as insertion sequences, insertion sequence-based transposition units, and transposons, which constituted a total of three novel MDR regions, two in p675920-1 and another in p675920-2. Coexistence of two MDR plasmids p675920-1 and p675920-2 made K. pneumoniae 675920 tend to become extensively drug-resistant.

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“…The rmtB gene mainly encodes a 16S rRNA methyltransferase to mediate a high level of resistance to aminoglycoside antibiotics ( 37 ). We observed that the rmtB gene is commonly located downstream of the transposon Tn 2 to form the Tn 2 - rmtB component, which is related to the bla TEM-1 gene and positioned upstream of rmtB ( 38 ). Our analysis described above shows that bla KPC-2 , bla CTX-M-65 , bla TEM-1 , and rmtB were all carried by typical mobile genetic elements, which potentially contributed to the dissemination of resistance genes.…”

Section: Discussionmentioning

confidence: 99%

Coexistence of Multidrug Resistance and Virulence in a Single Conjugative Plasmid from a Hypervirulent Klebsiella pneumoniae Isolate of Sequence Type 25

Xia

Miao

Yuan

et al. 2022

mSphere

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Frequent Tn 2 Misannotation in the Genetic Background of rmtB

Cited by 4 publications

References 14 publications

Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa

Characterization and Implications of IncP-2A Plasmid pMAS152 Harboring Multidrug Resistance Genes in Extensively Drug-Resistant Pseudomonas aeruginosa

WITHDRAWN: Structure genomics of two chimera plasmids p675920-1 and p675920-2 coexisting in a multi-drug resistant Klebsiella pneumoniae isolate

Coexistence of Multidrug Resistance and Virulence in a Single Conjugative Plasmid from a Hypervirulent Klebsiella pneumoniae Isolate of Sequence Type 25

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