FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan

Kubota, Hiroaki; Uwamino, Yoshifumi; Matsui, Mari; Sekizuka, Tsuyoshi; Suzuki, Yasunori; Okuno, Rumi; Uchitani, Yumi; Ariyoshi, Tsukasa; Aoki, Wataru; Suzuki, Satowa; Kuroda, Makoto; Shinkai, Takayuki; Yokoyama, Keiko; Sadamasu, Kenji; Funakoshi, Takeru; Murata, Mitsuru; Hasegawa, Naoki; Iwata, Satoshi

doi:10.1093/jac/dky291

Cited by 13 publications

(7 citation statements)

References 13 publications

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“…In 2010, the CDC reported the first carriage of NDM-1-producing E. cloacae in patients previously treated in India (208,209). Recently, metallo-␤-lactamases that comprise IMP-type enzymes and NDM-, GIM-, VIM-, and serine-carbapenemasetype KPC and FRI have been characterized (24,210,211). OXA-48-type serine carbapenemase seems to be the most prevalent (206,212).…”

Section: Molecular Mechanisms Of Resistancementioning

confidence: 99%

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

2019

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SUMMARY The genus Enterobacter is a member of the ESKAPE group, which contains the major resistant bacterial pathogens. First described in 1960, this group member has proven to be more complex as a result of the exponential evolution of phenotypic and genotypic methods. Today, 22 species belong to the Enterobacter genus. These species are described in the environment and have been reported as opportunistic pathogens in plants, animals, and humans. The pathogenicity/virulence of this bacterium remains rather unclear due to the limited amount of work performed to date in this field. In contrast, its resistance against antibacterial agents has been extensively studied. In the face of antibiotic treatment, it is able to manage different mechanisms of resistance via various local and global regulator genes and the modulation of the expression of different proteins, including enzymes (β-lactamases, etc.) or membrane transporters, such as porins and efflux pumps. During various hospital outbreaks, the Enterobacter aerogenes and E. cloacae complex exhibited a multidrug-resistant phenotype, which has stimulated questions about the role of cascade regulation in the emergence of these well-adapted clones.

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Section: Molecular Mechanisms Of Resistancementioning

confidence: 99%

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

2019

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“…Limited reports on FRI-type carbapenemases have been published since its description in 2015 and include those from the United Kingdom (FRI-2), 4 Germany (FRI-3), 5 Japan (FRI-4, FRI-5, FRI-8), 6–8 and Canada (FRI-6). 9 In addition, a report from 2019 described a novel carbapenemase FLC-1 (FRI-like carbapenemase) that shared 87% amino acid similarity to FRI-5 10 and 99% similarity to FRI-8.…”

Section: Brief Reportmentioning

confidence: 99%

Genetic and Phenotypic Characterization of the First Canadian Case of Ambler Class A Carbapenemase FRI-8

Mataseje

Doualla-Bell

Boyd

et al. 2023

Microbial Drug Resistance

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This study investigated the mechanism of carbapenem resistance in an Enterobacter cloacae complex positive by the modified carbapenem inactivation method (mCIM) but negative by the Rosco Neo-Rapid Carb Kit, β CARBA, and conventional PCR for common carbapenemase genes (KPC, NDM, OXA-48, IMP, VIM, GES, and IMI/NMC). Using whole genome sequencing (WGS) data we confirmed the identification of Enterobacter asburiae (ST1639) and the presence of bla FRI-8 located on a 148kb IncFII(Yp) plasmid. This is the first occurrence of a clinical isolate harboring the FRI-8 carbapenemase and the second occurrence of FRI in Canada. This study highlights the need to use both WGS and phenotypic screening methods for detection of carbapenemase-producing strains if we consider the growing diversity of carbapenemases.

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“…Pulsed-field gel electrophoresis using an S1-nuclease-digested DNA plug (S1-PFGE) showed that TA6363 carried three plasmids (Fig. 1), with the second larger one (pTMTA63632; ∼90 kbp) harboring the novel bla IMP gene according to sequencing plasmids extracted from the S1-PFGE gel (MiSeq; Illumina, San Diego, CA, USA) (9, 10). We found that this bla IMP gene encoded a Ser262Gly point mutant of IMP-11 metallo-β-lactamase, and we named this novel variant “ bla IMP-68 ” (see Fig.…”

Section: Observationmentioning

confidence: 99%

“…Additionally, we tested bla IMP-11 for comparison, and conjugation transfer was tested using E. coli J53, as previously described (9). The MICs of β-lactams were determined by the dry strip method using Etest.…”

Section: Observationmentioning

confidence: 99%

IMP-68, a Novel IMP-Type Metallo-β-Lactamase in Imipenem-Susceptible Klebsiella pneumoniae

Kubota

Suzuki

Okuno

et al. 2019

mSphere

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IMP-type metallo-β-lactamases comprise one group of the “Big 5” carbapenemases. Here, a novel blaIMP-68 gene encoding IMP-68 (harboring a Ser262Gly point mutant of IMP-11) was discovered from meropenem-resistant but imipenem-susceptible Klebsiella pneumoniae TA6363. The Ser262Gly substitution was previously identified as important for substrate specificity according to a study of other IMP variants, including IMP-6. We confirmed that IMP-68 exhibited weaker imipenem-hydrolyzing activity than that for other carbapenems, demonstrating that the antimicrobial susceptibility profile of TA6363 originated from IMP-68 substrate specificity, with this likely to affect treatment strategies using antibacterial agents in clinical settings. Notably, the carbapenem resistance conferred by IMP-68 was undetectable based on the MIC of imipenem as a carbapenem representative, which demonstrates a comparable antimicrobial susceptibility profile to IMP-6-producing Enterobacteriaceae that previously spread in Japan due to lack of awareness of its existence.

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FRI-4 carbapenemase-producing Enterobacter cloacae complex isolated in Tokyo, Japan

Abstract: The FRI-4-producing E. cloacae complex was isolated in Japan, whereas all other FRI variants have been found in Europe, suggesting that the spread of FRI carbapenemases is global.

Cited by 13 publications

References 13 publications

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

Enterobacter spp.: Update on Taxonomy, Clinical Aspects, and Emerging Antimicrobial Resistance

Genetic and Phenotypic Characterization of the First Canadian Case of Ambler Class A Carbapenemase FRI-8

IMP-68, a Novel IMP-Type Metallo-β-Lactamase in Imipenem-Susceptible Klebsiella pneumoniae

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