From Cascaded Catalytic Nucleic Acids to Enzyme–DNA Nanostructures: Controlling Reactivity, Sensing, Logic Operations, and Assembly of Complex Structures

Wang, Fuan; Lü, Chunhua; Willner, Itamar

doi:10.1021/cr400354z

Cited by 596 publications

(424 citation statements)

References 919 publications

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“…HCR draws on principles from the emerging disciplines of molecular programming and dynamic nucleic acid nanotechnology to provide isothermal enzyme-free signal amplification in diverse technological settings Wang et al, 2014;Jung and Ellington, 2014;Ikbal et al, 2015) and it is particularly wellsuited to the demands of in situ amplification (Choi et al, 2010(Choi et al, , 2014.…”

Section: Resultsmentioning

confidence: 99%

Mapping a multiplexed zoo of mRNA expression

et al. 2016

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In situ hybridization methods are used across the biological sciences to map mRNA expression within intact specimens. Multiplexed experiments, in which multiple target mRNAs are mapped in a single sample, are essential for studying regulatory interactions, but remain cumbersome in most model organisms. Programmable in situ amplifiers based on the mechanism of hybridization chain reaction (HCR) overcome this longstanding challenge by operating independently within a sample, enabling multiplexed experiments to be performed with an experimental timeline independent of the number of target mRNAs. To assist biologists working across a broad spectrum of organisms, we demonstrate multiplexed in situ HCR in diverse imaging settings: bacteria, whole-mount nematode larvae, whole-mount fruit fly embryos, whole-mount sea urchin embryos, whole-mount zebrafish larvae, whole-mount chicken embryos, whole-mount mouse embryos and formalin-fixed paraffinembedded human tissue sections. In addition to straightforward multiplexing, in situ HCR enables deep sample penetration, high contrast and subcellular resolution, providing an incisive tool for the study of interlaced and overlapping expression patterns, with implications for research communities across the biological sciences.

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Section: Resultsmentioning

confidence: 99%

Mapping a multiplexed zoo of mRNA expression

et al. 2016

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“…Fig. 5 (a) shows the effect of typical group analysis and evaluation method based Formula (1) that proposed in literature (Wang, Lu and Willner, 2014). rw c evaluates the recommended capacity of rater.…”

Section: Experimental Platformmentioning

confidence: 99%

Group Analysis and Evaluation Method of Enterprise Competitiveness Based on Law of Logic Partial Order

2017

Revista Tecnica De La Facultad De Ingenieria Universidad Del Zu

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The node in the network of enterprise competitiveness is possible to receive service and information with poor quality from the other nodes. Group analysis and evaluation is a common method to solve this problem. The calculation mechanism of enterprise competitiveness based on score feedback has the following disadvantages: unable to distinguish the difference between malicious evaluation and error evaluation given by honest nodes; need second evaluation on the score credibility, which slows down the computing speed of competitiveness; the method of using number to express the node reputation is not natural. In fact, the use of group analysis and evaluation is to determine the relative order of node credibility. Therefore, a kind of reputation based law of logic partial order (RLPO) was proposed. The mathematical modeling and theoretical analysis were conducted based on RLPO and its malicious attacks. Results show that the influence of non-malicious error of RLPO decreases with the exponent of law of logic partial order number; generally, the effect of malicious attack on RLPO decreases with the polynomial of law of logic partial order number; for the collusion attack by design, the malicious attacks were effectively neutralized because RLPO must introduce the right information. Therefore, RLPO no longer feeds back rating information. So there is no group analysis and evaluation problem caused by score feedback (such as no need of second evaluation for the credibility of feedback information).

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“…The objective of this section is therefore not to present an exhaustive list, already published (Jung and Ellington 2014;Wang et al 2014;Gerasimova and Kolpashchikov 2014), but to present how advances made in the context of nucleotide sequence detection can be cleverly combined with aptamer selectivity.…”

Section: Amplification Strategies and Aptamersmentioning

confidence: 99%