From global proteome profiling to single targeted molecules of follicular fluid and oocyte: contribution to embryo development and IVF outcome

Benkhalifa, Moncef; Madkour, Aicha; Louanjli, Noureddine; Bouamoud, Nouzha; Saadani, Brahim; Kaarouch, Ismail; Chahine, Hikmat; Sefrioui, O; Merviel, Philippe; Copin, Henri

doi:10.1586/14789450.2015.1056782

Cited by 31 publications

(20 citation statements)

References 158 publications

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“…In the postgenomic era, proteomic techniques have been widely used in the field of reproductive medicine. HFF proteome has become a hotspot for research, which not only contributed to discovering proteins related to IVF outcomes, but also improved our comprehensive understanding of physiological process during follicle development and oocyte maturation [ 11 ]. Li and co-workers used surface-enhanced laser desorption/ionization-time of flight-mass spectrometry (SELDI-TOF-MS) combined with weak cation-exchange protein chip (WCX-2) to search for differentially expressed HFF proteins from mature and antral follicles [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Shen

Liu

Zhu

et al. 2017

Reprod Biol Endocrinol

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BackgroundHuman follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome.MethodsTo better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF.ResultsA total of 219 unique high-confidence (False Discovery Rate (FDR) < 0.01) HFF proteins were identified by searching the reviewed Swiss-Prot human database (20,183 sequences), and MS data were further verified by western blot. PANTHER showed HFF proteins were involved in complement and coagulation cascade, growth factor and hormone, immunity, and transportation, KEGG indicated their pathway, and STRING demonstrated their interaction networks. In comparison, 32% and 50% of proteins have not been reported in previous human follicular fluid and plasma.ConclusionsOur HFF proteome research provided a new complementary high-confidence dataset of folliculogenesis and oocyte maturation environment. Those proteins associated with innate immunity, complement cascade, blood coagulation, and angiogenesis might serve as the biomarkers of female infertility and IVF outcome, and their pathways facilitated a complete exhibition of reproductive process.Electronic supplementary materialThe online version of this article (doi:10.1186/s12958-017-0277-y) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Shen

Liu

Zhu

et al. 2017

Reprod Biol Endocrinol

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“…Human follicular fluid (HFF) provides a special and ideal microenvironment for oocyte maturation, composed of steroid hormones, metabolites and proteins, which are required for follicle development and oocyte maturation [7][8][9]. Importantly, HFF can be easily obtained during the aspiration of oocytes from follicle in the procedure of in vitro fertilization (IVF), which facilitates it to be as a useful source for the biomarker screening for oocyte maturation [10]. Proteomic technology has been applied to explore key molecules for the better understanding of the physiological processes involved in follicle development and oocyte maturation.…”

Section: Open Accessmentioning

confidence: 99%

Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

et al. 2020

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Background: Human follicular fluid (HFF), which is composed by essential proteins required for the follicle development, provides an important microenvironment for oocyte maturation. Recently, overweight status has been considered as a detrimental impact factor on oocyte maturation, but whether HFF proteome could provide protein markers for assessing overweight-based oocyte maturation deficiency is still unknown. Methods: To reveal the HFF-based molecular characteristics associated with abnormal oocyte maturation, an iTRAQbased comparative proteomic analysis was performed to investigate different HFF protein expression profiles from normal weight women and overweight status women. Results: Two hundred HFF proteins were quantified in our data, of which 43% have not been overlapped by two previous publications. Compared with the HFF proteins of normal weight women, 22 up-regulated HFF proteins and 21 down-regulated HFF proteins were found in the overweight status women. PANTHER database showed these altered HFF proteins participated in development, metabolism, immunity, and coagulation, and STRING database demonstrated their complicated interaction networks. The confidence of proteomic outcome was verified by Western blot analysis of WAP four-disulfide core domain protein 2 (WFDC2), lactotransferrin (LTF), prostate-specific antigen (KLK3), fibronectin (FN1), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH). Further, ELISA assay indicated WFDC2 might be a potentially useful candidate HFF marker for the diagnosis of oocyte maturation arrest caused by overweight status. Conclusions: Our work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women.

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“…Proteins being translated from RNA are directly responsible for cellular function, but different gene expression studies have, unfortunately, shown that a reliable prediction of proteins' function or abundance cannot be made. As for the human proteome, significant advances have been made for the analysis of the whole proteome and of the proteome of different disease states [3,5,[10][11][12][13][14][15][16][17][18][19][20]. Bearing all of it in mind, the analysis of proteins obtained from the cultivating media is challenging, and the results are not always conclusive.…”

Section: Albumin Depletion In Ivf Cultivation Mediummentioning

confidence: 99%

Proteomics as a Future Tool for Improving IVF Outcome

Mitulović¹,

Panić-Janković²

2020

Innovations in Assisted Reproduction Technology

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New technical and methodical and more efficient approaches beyond preimplantation genetic screening (PGS) are needed to elevate success rates in in vitro fertilization (IVF). One new approach could be the characterization of the embryos' proteome during the IVF process. This means that specific proteins secreted by the embryo in the surrounding cultivating medium can be analyzed and compared between embryos in order to identify potential markers for a successful embryo transfer and resulting pregnancy. Furthermore, this procedure could result with understanding the processes during the whole time of incubation, from the moment of oocyte fertilization until embryo transfer and subsequently implantation by analyzing the culturing medium used in multiple culture medium exchange during the cultivation period. This procedure of embryo transfer to a new culture medium is essential for the embryo's development and is performed daily or at least when the embryos reached the stage of embryoblast at day 4. The remaining medium after embryo removal is routinely discarded. However, this medium still can be useful for a detailed analysis of proteins and lipids that were secreted by the embryo during the previous incubation process and could help gaining information on the embryos' current developmental status.

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From global proteome profiling to single targeted molecules of follicular fluid and oocyte: contribution to embryo development and IVF outcome

Cited by 31 publications

References 158 publications

Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality

Proteomics as a Future Tool for Improving IVF Outcome

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