From molecular genetics to functional genomics and physiome

Haulică, I

doi:10.1111/j.1582-4934.2002.tb00463.x

Cited by 3 publications

(2 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Traditional anti-gene agents, antisense oligonucleotides and ribozymes, and the new promising anti-gene strategy, RNA interference, which disrupt the expression of diseaserelated genes, could potentially be of utility not only in the treatment of allergy, but also in the therapeutics of many other diseases, including cancer [69,[70][71][72][73][74][75][76][77].…”

Section: Discussionmentioning

confidence: 99%

Antisense- and RNA interference-based therapeutic strategies in allergy

Popescu¹

2005

J Cellular Mol Med

View full text Add to dashboard Cite

Modern therapeutic methods for manipulation of gene expression in allergic diseases have been receiving increased attention in the emerging era of functional genomics. With the growing application of gene silencing technologies, pharmacological modulation of translation represents a great advance in molecular therapy for allergy. Several strategies for sequence-specific post-transcriptional inhibition of gene expression can be distinguished: antisense oligonucleotides (AS-ONs), ribozymes (RZs), DNA enzymes (DNAzymes), and RNA interference (RNAi) triggered by small interfering RNAs (siRNAs). Potential anti-mRNA drugs in asthma and other allergic disorders may be targeted to cell surface receptors (adenosine A 1 receptor, high-affinity receptor Fc-ε RI-α, cytokine receptors), adhesion molecules and ligands (ICAM-1, VLA-4), ion channels (calcium-dependent chloride channel-1), cytokines and related factors (IL-4, IL-5, IL-13, SCF, TNF-α, TGF-β1), intracellular signal transduction molecules, such as tyrosine-protein kinases (Syk, Lyn, Btk), serine/threonine-protein kinases (p38 α MAP kinase, Raf-1), non-kinase signaling proteins (RasGRP4), and transcription factors involved in Th2 differentiation and allergic inflammation (STAT-6, GATA-3, NF-κB). The challenge to scientists is to determine which of the candidate targets warrants investment of time and resources. New-generation respirable AS-ONs, external guide sequence ribozymes, and RNA interference-based therapies have the potential to satisfy unmet needs in allergy treatment, acting at a more proximal level to a key etiopathogenetic molecular process, represented by abnormal expression of genes. Moreover, antisense and siRNA technologies imply a more rational design of new drugs for allergy.

show abstract

Section: Discussionmentioning

confidence: 99%

Antisense- and RNA interference-based therapeutic strategies in allergy

Popescu¹

2005

J Cellular Mol Med

View full text Add to dashboard Cite

show abstract

“…From molecular genetics to functional genomics and proteomics, a whole range of data are still brought together to decipher the impact of time-set mechanisms upon basic mechanisms of life. The ion channels activity, transcription and translation, various phosphorylation processes are involved in this circadian clockweb [53].…”

Section: Cardiovascular and Cerebrovascular Pathophysiologymentioning

confidence: 99%

Genetic clock of biologic rhythms

Badiu

2003

J Cellular Molecular Medi

View full text Add to dashboard Cite

Biologic rhythms are tighly regulated in all species, in order to addapt to circadian variations of light, temperature and other biological related living conditions. The intrinsic mechanisms of rhythm regulation from every cell to a whole organism is embedded into the genetic clockwork. The mammalian clock genes are reviewed with their interrelation. The per gene promoter is stimulated by clock/bmall heterodimer and trigger the per protoeins synthesis. Innitialy considered as a simple negative feed‐back, from to the phosphorylated per/cry heterodimer towards clock/bmall transcription stimulus, the clockweb of the cell became complex. General characteristics of cell clock genes are integrated in a model. In addition, the involvement of clock genes in peripheral tissues is discussed.

show abstract

New Challenges and Perspectives in Physiology. From the Classical Concept of Homeostasis to Physiome

Haulică¹

2005

Acta Endo (Buc)

View full text Add to dashboard Cite

From molecular genetics to functional genomics and physiome

Cited by 3 publications

References 19 publications

Antisense- and RNA interference-based therapeutic strategies in allergy

Antisense- and RNA interference-based therapeutic strategies in allergy

Genetic clock of biologic rhythms

New Challenges and Perspectives in Physiology. From the Classical Concept of Homeostasis to Physiome

Contact Info

Product

Resources

About