From Pauling's abzyme concept to the new era of hydrolytic anti-DNA autoantibodies: a link to rational vaccine design? &amp;ndash; A review

Pavlović, Mirjana; Cavallo, Michelle; Kats, A. M.; Kotlarchyk, Alex; Zhuang, Hanqi; Shoenfeld, Yehuda

doi:10.1504/ijbra.2011.041735

Cited by 7 publications

(4 citation statements)

References 29 publications

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“…Lupus anti-DNA antibodies demonstrate the ability to penetrate living cells via receptors such as Myosin I, to enter the nucleus, and to trigger cell death in vitro. Hydrolytic anti-DNA antibody could potentially maintain and perpetuate disease by DNA hydrolysis in the nuclei of penetrated cells [36–42]. Future analyses should include cytotoxicity studies and fluorescence-aided tracking of purified, natural hydrolytic antibodies obtained and investigated by the herein described methods.…”

Section: Discussionmentioning

confidence: 99%

A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

Cavallo

Kats

Chen

et al. 2012

Autoimmune Diseases

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Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of antibodies against a variety of self-antigens including nucleic acids. These antibodies are cytotoxic, catalytic (hydrolyzing DNA, RNA, and protein), and nephritogenic. Current methods for investigating catalytic activities of natural abzymes produced by individuals suffering from autoimmunity are mostly discontinuous and often employ hazardous reagents. Here we demonstrate the utility of dual-labeled, fluorogenic DNA hydrolysis probes in highly specific, sensitive, continuous, fluorescence-based measurement of DNA hydrolytic activity of anti-ssDNA abzymes purified from the serum of patients suffering from SLE. An assay for the presence and levels of antibodies exhibiting hydrolytic activity could facilitate disease diagnosis, prediction of flares, monitoring of disease state, and response to therapy. The assay may allow indirect identification of additional targets of anti-DNA antibodies and the discovery of molecules that inhibit their activity. Combined, these approaches may provide new insights into molecular mechanisms of lupus pathogenesis.

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Section: Discussionmentioning

confidence: 99%

A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

Cavallo

Kats

Chen

et al. 2012

Autoimmune Diseases

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“…In addition, bioinformatics methods can help understand the specificity of catalytic antibodies to assist repeated tests in the laboratory, thus saving time and money. The related tools are applicable for autoimmune analysis of binding sites, prediction of binding activity and most likely motifs of antibody binding, and dynamics simulation of catalytic reaction [ 41 , 48 , 86 – 88 ]. Luo designed the new catalytic ability into the antibody scFv2F3 by combining computational design and site-directed mutagenesis [ 89 ].…”

Section: Other Methods Of Producing Catalytic Antibodiesmentioning

confidence: 99%

Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Zhao

Chen

et al. 2022

Appl Biochem Biotechnol

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Catalytic antibodies made it feasible to develop new catalysts, which had previously been the subject of research. Scientists have discovered natural antibodies that can hydrolyze substrates such as nucleic acids, proteins, and polysaccharides during decades of research, as well as several ways of producing antibodies with specialized characteristics and catalytic functions. These antibodies are widely used in chemistry, biology, and medicine. Catalytic antibodies can continue to play a role and even fully prevent the emergence of autoimmune disorders, especially in the field of infection and immunity, where the process of its occurrence and development often takes a long time. In this work, the development, design and evolution methodologies, and the expression systems and applications of catalytic antibodies, are discussed. Trial registration: not applicable.

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“…The purpose of knowing correct and aberrant splice-junctions in the context of viral DNA (such as DEN 1 virus) is pertinent to and implicates vaccine designs [14]. In general, a gene is first transcribed into pre-mRNA, which is a copy of genomic DNA containing exon and intron regions.…”

Section: Depicted Inmentioning

confidence: 99%

Fuzzy splicing in precursor-mRNA sequences: prediction of aberrant splice-junctions in viral DNA context

Neelakanta¹,

Chatterjee²,

Pavlović³

et al. 2011

JBiSE

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RNA splicing normally generates stable splice-junction sequences in viruses that are important in the context of virus mimicry. Potential variability in envelop proteins may occur with point-mutations inducing cryptic splice-junctions, which would remain unrecognized by T-memory cells of higher organisms in vaccine trials. Such aberrant splicejunctions result from evolution-specific non-conservation of actual splice-junction sites due to mutations; as such, locations of splice-junctions in a test DNA sequence could only be imprecisely specified. Such impreciseness of splice-junction locations (or cryptic sites) in a sequence is evaluated in this study via "noisy" attributes (with associated stochastics) to the mutated subspace; and, relevant fuzzy considerations are invoked with membership attributes expressed in terms of a spatial signal-to-noise ratio (SSNR). That is, SSNR adopted as a membership function expresses the belongingness of a site-region to exon/intron subspaces. An illustrative example with actual (Dengue 1 viral) DNA data is furnished demonstrating the pursuit developed in predicting aberrant splice-junctions at cryptic sites in the test sequence.

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From Pauling's abzyme concept to the new era of hydrolytic anti-DNA autoantibodies: a link to rational vaccine design? – A review

Cited by 7 publications

References 29 publications

A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

A Novel Method for Real-Time, Continuous, Fluorescence-Based Analysis of Anti-DNA Abzyme Activity in Systemic Lupus

Catalytic Antibodies: Design, Expression, and Their Applications in Medicine

Fuzzy splicing in precursor-mRNA sequences: prediction of aberrant splice-junctions in viral DNA context

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