From physiology to systems metabolic engineering for the production of biochemicals by lactic acid bacteria

BacHBerry: BACterial Hosts for production of Bioactive phenolics from bERRY fruits

“…acetaldehyde and diacetyl), and vitamins. Furthermore, production of plant secondary metabolites, namely isoprenoids and stilbenes, has been demonstrated in this organism (Neves et al 2005;Katz et al 2011;Gaspar et al 2013;Song et al 2014). In addition, L. lactis is well known as an excellent host for the expression of both prokaryotic and eukaryotic proteins, and has been consequently used for development of live vaccine (Kunji et al 2005;Hernández et al 2007;Frelet-Barrand et al 2010;Pontes et al 2011).…”

“…Both L. lactis and C. glutamicum are used worldwide in numerous industrial processes, which consequently led to significant amount of research work dedicated towards improving our knowledge of their physiology and genetics. These efforts have resulted in development of large toolboxes for (heterologous) gene expression and introduction of genetic modifications in these bacteria (Burkovski 2008;Pontes et al 2011;Gaspar et al 2013). Furthermore, high-throughput methodologies for transcriptome, proteome and metabolome analysis, and genome-scale metabolic models are available for these species (Krömer et al 2004;Oliveira et al 2005;Shinfuku et al 2009;Gaspar et al 2013).…”

“…These efforts have resulted in development of large toolboxes for (heterologous) gene expression and introduction of genetic modifications in these bacteria (Burkovski 2008;Pontes et al 2011;Gaspar et al 2013). Furthermore, high-throughput methodologies for transcriptome, proteome and metabolome analysis, and genome-scale metabolic models are available for these species (Krömer et al 2004;Oliveira et al 2005;Shinfuku et al 2009;Gaspar et al 2013). Combined with their general robustness, ease of handling and the GRAS status, both L. lactis and C. glutamicum have a strong potential for becoming platform organisms for production of polyphenolic compounds.…”

“…It is a low G ? C aerotolerant bacterium with a relatively small genome and simple sugar metabolism: utilization occurs via glycolysis (Embden-Meyerhof-Parnas pathway) with lactic acid being the major end-product (Gaspar et al 2013). Its ability to ferment various carbohydrates, such as hexoses, pentoses and, in some strains, even complex carbohydrates of plant origin, as well as its high alcohol, acid and stress tolerance makes this bacterium suitable for the harsh environments commonly found under industrial production conditions.…”

Excess of threonine compared with serine promotes threonine aldolase activity in Lactococcus lactis IL1403

“…Moreover, the technological advances in the last decade have created the opportunity to exploit LAB for the bioproduction of value-added chemicals, e.g. vitamins, polysaccharides, low-calorie sweeteners (polyols), flavour compounds and ethanol (Gaspar et al, 2013).…”