From procambium patterning to cambium activation and maintenance in the Arabidopsis root

Wang, Xin; Mäkilä, Riikka; Mähönen, Ari Pekka

doi:10.1016/j.pbi.2023.102404

Cited by 6 publications

(2 citation statements)

References 120 publications

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“…promotes differentiation and/or maintenance of the vascular procambium. Down-regulation of WOX4 in Arabidopsis generated small plants with undifferentiated ground tissue and exhibited severe reductions in differentiated xylem and phloem [58]. The genome editing approach for this gene would facilitate uniform patterning in Eastern carrot cvs.…”

Section: Grn Associated With Vc Patterning Between Test Carrot Cultivarsmentioning

confidence: 99%

Comparative Root Transcriptome Profiling and Gene Regulatory Network Analysis between Eastern and Western Carrot (Daucus carota L.) Cultivars Reveals Candidate Genes for Vascular Tissue Patterning

Kulkarni,

Cholin,

Bajpai

et al. 2023

Plants

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Carrot (Daucus carota L.) is a highly consumed vegetable rich in carotenoids, known for their potent antioxidant, anti-inflammatory, and immune-protecting properties. While genetic and molecular studies have largely focused on wild and Western carrot cultivars (cvs), little is known about the evolutionary interactions between closely related Eastern and Western cvs. In this study, we conducted comparative transcriptome profiling of root tissues from Eastern (UHSBC-23-1) and Western (UHSBC-100) carrot cv. to better understand differentially expressed genes (DEGs) associated with storage root development and vascular cambium (VC) tissue patterning. Through reference-guided TopHat mapping, we achieved an average mapping rate of 73.87% and identified a total of 3544 DEGs (p < 0.05). Functional annotation and gene ontology classification revealed 97 functional categories, including 33 biological processes, 19 cellular components, 45 metabolic processes, and 26 KEGG pathways. Notably, Eastern cv. exhibited enrichment in cell wall, plant-pathogen interaction, and signal transduction terms, while Western cv. showed dominance in photosynthesis, metabolic process, and carbon metabolism terms. Moreover, constructed gene regulatory network (GRN) for both cvs. obtained orthologs with 1222 VC-responsive genes of Arabidopsis thaliana. In Western cv, GRN revealed VC-responsive gene clusters primarily associated with photosynthetic processes and carbon metabolism. In contrast, Eastern cv. exhibited a higher number of stress-responsive genes, and transcription factors (e.g., MYB15, WRKY46, AP2/ERF TF connected via signaling pathways with NAC036) were identified as master regulators of xylem vessel differentiation and secondary cell wall thickening. By elucidating the comparative transcriptome profiles of Eastern and Western cvs. for the first time, our study provides valuable insights into the differentially expressed genes involved in root development and VC tissue patterning. The identification of key regulatory genes and their roles in these processes represents a significant advancement in our understanding of the evolutionary relations and molecular mechanisms underlying secondary growth of carrot and regulation by vascular cambium.

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Section: Grn Associated With Vc Patterning Between Test Carrot Cultivarsmentioning

confidence: 99%

Comparative Root Transcriptome Profiling and Gene Regulatory Network Analysis between Eastern and Western Carrot (Daucus carota L.) Cultivars Reveals Candidate Genes for Vascular Tissue Patterning

Kulkarni,

Cholin,

Bajpai

et al. 2023

Plants

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show abstract

“…Pre-procambial initials start dividing at the globe stage, forming a radial pattern resembling post-embryonic root vasculature ( Rodriguez-Villalon et al., 2014 ). The signaling pathways regulating primary vascular development were discussed in several recent excellent review papers ( Fischer and Teichmann, 2017 ; Tonn and Greb, 2017 ; Wang, 2020 ; Turley and Etchells, 2022 ; Wang et al., 2023 ). This paper mainly focuses on advances in regulating plant vascular cambial activity and secondary growth.…”

Section: Introductionmentioning

confidence: 99%

Endogenous and environmental signals in regulating vascular development and secondary growth

Wang

2024

Front. Plant Sci.

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How Moving Hormonal Signals Regulate Plant Vascular Differentiation, Adaptation, and Evolution: Hypotheses and Evidence

Aloni

2023

Progress in Botany

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From procambium patterning to cambium activation and maintenance in the Arabidopsis root

Cited by 6 publications

References 120 publications

Comparative Root Transcriptome Profiling and Gene Regulatory Network Analysis between Eastern and Western Carrot (Daucus carota L.) Cultivars Reveals Candidate Genes for Vascular Tissue Patterning

Comparative Root Transcriptome Profiling and Gene Regulatory Network Analysis between Eastern and Western Carrot (Daucus carota L.) Cultivars Reveals Candidate Genes for Vascular Tissue Patterning

Endogenous and environmental signals in regulating vascular development and secondary growth

How Moving Hormonal Signals Regulate Plant Vascular Differentiation, Adaptation, and Evolution: Hypotheses and Evidence

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