From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis

Khan, Mohd. Kamran; Choudhary, Saumya; Hakkı, Erdoğan E.; Akkaya, Mahinur S.; Thomas, George

doi:10.1007/s10722-014-0114-5

Cited by 33 publications

(27 citation statements)

References 201 publications

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“…Marker systems based on different wheat properties like protein content, flour colour, gluten strength has facilitated the upgrading of durum quality (Nachit et al, 2001;Maccaferri et al, 2006). Other than these properties, genetic diversity studies of diverse accessions is strengthen by the advancement and thriving appliance of various types of molecular markers (Khan et al, 2014). For more than two decades, simple sequence repeats (SSRs) or microsatellite markers (Tautz et al, 1986) have been progressively used for diversity analysis of native and contemporary cultivars (Todorovska et al, 2005;Al Khanjari et al, 2007;Colomba and Gregorini, 2011) and has shown promising polymorphism.…”

Section: Detection Of Microsatellitesmentioning

confidence: 99%

Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

HAKKI¹,

DOGRAR²,

PANDEY³

et al. 2014

Not Bot Hort Agrobot Cluj

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Combination of elemental and protein studies along with molecular data using microsatellite markers may lead to the better and realistic determination of relatedness between the varieties and their populations. In this study, the extent of diversity among five Turkish durum wheat cultivars and their populations has been assessed using seven microsatellite markers and the elemental analysis together with the differences in their protein content. In molecular analysis, total 23 alleles have been obtained among all the genotypes with middling of 4.6 per primer. On employing UPGMA Dendrogram, Principle Coordinate Analysis (PCoA) and Winboot analyses, both inter and intra varietal polymorphic studies had shown similar clustering with minor differences. As a result of AMOVA performed, the extent of diversity was found to be higher among the genotypes (76%) in comparison to the variability within the genotypes (24%). In elemental analyses, 'Selcuklu-97' was found to be the most efficient variety with high content of several elements. Also, strong and positive correlation has been observed between magnesium-phosphorus, magnesium-sulphur and sulphur-sodium, while noteworthy negative correlation has been observed between sodium and zinc. The protein content of the genotypes was found in the range of 15.17-16.90%. The diversity revealed in durum genotypes can be employed in genetic expansion of the crop. These involved varieties may aid to avoid genetic attrition coming up from the landraces. The information provided can be utilized by breeders for appropriate selection of both, genetically and nutritionally efficient durum wheat varieties.

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Section: Detection Of Microsatellitesmentioning

confidence: 99%

Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

HAKKI¹,

DOGRAR²,

PANDEY³

et al. 2014

Not Bot Hort Agrobot Cluj

Self Cite

View full text Add to dashboard Cite

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“…Efforts have been made over the decades to study the genetic diversity of crop population (Fu 2015;Govindaraj et al 2015). Various molecular methods have also been developed for genetic analysis of wheat populations (Khan et al 2014). The potential contribution of main generation molecular markers and their applications have been extensively reviewed (Landjeva et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Structure of Mongolian Wheat Based on SSR Markers: Implications for Conservation and Management

Ya¹,

Raveendar²,

Bayarsukh³

et al. 2017

Plant Breed. Biotech.

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Production of spring wheat, the major crop in Mongolia, accounts for 98% of the cultivated area. Understanding genetic variability in existing gene bank accessions is critical for collection, conservation and use of wheat germplasms. To determine genetic diversity and population structure among a representative collection of Mongolian local wheat cultivars and lines, 200 wheat accessions were analyzed with 15 SSR markers distributed throughout the wheat genome. A total of 85 alleles were detected, with three to five alleles per locus and a mean genetic richness of 5.66. Average genetic diversity index was 0.69, with values ranging from 0.37-0.80. The 200 Mongolian wheat accessions were mainly divided into two subgroups based on structure and phylogenetic analyses, and some phenotypes were divergent by the subgroups. Results from this study will provide valuable information for conservation and sustainable use of Mongolian wheat genetic resources.

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“…Despite the development of the next-generation sequencing, the DNA fingerprinting is still the preferred way of plant genetic resources (PGR) genetic characterization [10–13]. Molecular biology provides various methods based on the DNA polymorphism which are not affected by the environment; they require only a small amounts of biological material at any growth stage that can be easily transported from the collection site to a laboratory and stored for a long time [14].…”

Section: Introductionmentioning

confidence: 99%

“…In genetic diversity studies, many different types of molecular markers have been used, including RFLP (Restriction Fragments Length Polymorphism), RAPD (Random Amplified Polymorphic DNA), AFLP (Amplified Fragment Length Polymorphism), ISSR (Inter-Simple Sequence Repeats), SSR (Simple Sequence Repeats), CAPS (Cleaved Amplified Polymorphic Sequences), SNP (Single Nucleotide Polymorphism), etc. [10,16–18]. ISSR is cheap and effective genotyping technique that have been used for determining genetic diversity [19,20].…”

Section: Introductionmentioning

confidence: 99%

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

et al. 2016

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The assessment of diversity and population structure and construction of a core collection is beneficial for the efficient use and management of germplasm. A unique collection of common oat landraces, cultivated in the temperate climate of central Europe until the end of the twentieth century, is preserved in the Polish gene bank. It consists of 91 accessions that have never been used in breeding programs. In order to optimise the use of this genetic resource, we aimed to: (1) determine genetic and agro-morphological diversity, (2) identify internal genetic variation of the tested accessions, (3) form a core collection and (4) recognise the accessions useful for breeding programs or re-release for cultivation. The collection was screened using ISSR markers (1520 loci) and eight agro-morphological traits. Uniquely, we performed molecular studies based on 24 individuals of every accession instead of bulk samples. Therefore, assessment of the degree of diversity within each population and the identification of overlapping gene pools were possible. The observed internal diversity (Nei unbiased coefficient) was in the range of 0.17–0.31. Based on combined genetic and agro-morphological data, we established the core collection composed of 21 landraces. Due to valuable compositions of important traits, some accessions were also identified as useful for breeding programs. The population structure and principal coordinate analysis revealed two major clusters. Based on the previous results, the accessions classified within the smaller one were identified as obsolete varieties instead of landraces. Our results show that the oat landraces are, in general, resistant to local races of diseases, well adapted to local conditions and, in some cases, yielding at the level of modern varieties. Therefore, in situ conservation of the landraces in the near future may be satisfactory for both farmers and researchers in terms of the genetic resources preservation.

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From RFLP to DArT: molecular tools for wheat (Triticum spp.) diversity analysis

Cited by 33 publications

References 201 publications

Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

Molecular and Elemental Characterization of Selected Turkish Durum Wheat Varieties

Genetic Diversity and Population Structure of Mongolian Wheat Based on SSR Markers: Implications for Conservation and Management

Promoting the Use of Common Oat Genetic Resources through Diversity Analysis and Core Collection Construction

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