From spherical compartments to polymer films: exploiting vesicle fusion to generate solid supported thin polymer membranes

Kyropoulou, Myrto; Avsar, Saziye Yorulmaz; Schoenenberger, Cora‐Ann; Palivan, Cornelia G.

doi:10.1039/d1nr01122g

Cited by 8 publications

(8 citation statements)

References 72 publications

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“…However, within the 12-h period, we observed a slight size decrease to an average diameter of 91 ± 60 nm, which was accompanied by increased scattering intensity at a micrometer size range (>1 µm) ( Figure 3 , triangles). This result can be explained by a slow aggregation of the vesicles with time at this pH and settling down, as reported recently [ 51 ], which agrees with the data on the average size and size distribution decrease.…”

Section: Resultssupporting

confidence: 92%

Dually Responsive Poly(N-vinylcaprolactam)-b-poly(dimethylsiloxane)-b-poly(N-vinylcaprolactam) Polymersomes for Controlled Delivery

et al. 2022

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Limited tissue selectivity and targeting of anticancer therapeutics in systemic administration can produce harmful side effects in the body. Various polymer nano-vehicles have been developed to encapsulate therapeutics and prevent premature drug release. Dually responsive polymeric vesicles (polymersomes) assembled from temperature-/pH-sensitive block copolymers are particularly interesting for the delivery of encapsulated therapeutics to targeted tumors and inflamed tissues. We have previously demonstrated that temperature-responsive poly(N-vinylcaprolactam) (PVCL)-b-poly(dimethylsiloxane) (PDMS)-b-PVCL polymersomes exhibit high loading efficiency of anticancer therapeutics in physiological conditions. However, the in-vivo toxicity of these polymersomes as biocompatible materials has not yet been explored. Nevertheless, developing an advanced therapeutic nanocarrier must provide the knowledge of possible risks from the material’s toxicity to support its future clinical research in humans. Herein, we studied pH-induced degradation of PVCL10-b-PDMS65-b-PVCL10 vesicles in-situ and their dually (pH- and temperature-) responsive release of the anticancer drug, doxorubicin, using NMR, DLS, TEM, and absorbance spectroscopy. The toxic potential of the polymersomes was evaluated in-vivo by intravenous injection (40 mg kg−1 single dose) of PVCL10-PDMS65-PVCL10 vesicles to mice. The sub-acute toxicity study (14 days) included gravimetric, histological, and hematological analyses and provided evidence for good biocompatibility and non-toxicity of the biomaterial. These results show the potential of these vesicles to be used in clinical research.

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Section: Resultssupporting

confidence: 92%

Dually Responsive Poly(N-vinylcaprolactam)-b-poly(dimethylsiloxane)-b-poly(N-vinylcaprolactam) Polymersomes for Controlled Delivery

et al. 2022

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“…QCM-D is commonly used in the analysis of vesicle adsorption on various substrates. [47][48][49] Reusability of our platform afforded by reversible hybridization of complementary DNA can be demonstrated using a silica-sputtered QCM sensor functionalized with NH 2 -DNA, which was further stabilized by the addition of a blocking agent. We developed a cyclic procedure that allows for reusing the DNA-microprinted surface (Figure 7E).…”

Section: Reusability Of a Cnc Platformmentioning

confidence: 99%

A DNA‐Micropatterned Surface for Propagating Biomolecular Signals by Positional on‐off Assembly of Catalytic Nanocompartments

Maffeis

Hürlimann

Krywko‐Cendrowska

et al. 2022

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Signal transduction is pivotal for the transfer of information between and within living cells. The composition and spatial organization of specified compartments are key to propagating soluble signals. Here, a high‐throughput platform mimicking multistep signal transduction which is based on a geometrically defined array of immobilized catalytic nanocompartments (CNCs) that consist of distinct polymeric nanoassemblies encapsulating enzymes and DNA or enzymes alone is presented. The dual role of single entities or tandem CNCs in providing confined but communicating spaces for complex metabolic reactions and in protecting encapsulated compounds from denaturation is explored. To support a controlled spatial organization of CNCs, CNCs are patterned by means of DNA hybridization to a microprinted glass surface. Specifically, CNC‐functionalized DNA microarrays are produced where individual reaction compartments are kept in close proximity by a distinct geometrical arrangement to promote effective communication. Besides a remarkable versatility and robustness, the most prominent feature of this platform is the reversibility of DNA‐mediated CNC‐anchoring which renders it reusable. Micropatterns of polymer‐based nanocompartment assemblies offer an ideal scaffold for the development of the next generation responsive and communicative soft‐matter analytical devices for applications in catalysis and medicine.

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“…Solid-supported polymer and lipid membranes are produced by Langmuir–Blodgett, 32 vesicle fusion, 33 , 34 in the form of polymer disks, 35 tethered bilayers, 36 , 37 and recently reported, solvent-assisted (SA) method. 38 − 40 Of particular interest is the SA method as it is straightforward and requires no special equipment or complex and time-consuming sample preparation conditions.…”

Section: Introductionmentioning

confidence: 99%

“…14,22,30 The architecture and properties of a hybrid membrane can be controlled by adjusting the miscibility/ T h i s c o n t e n t i s immiscibility effects that depend on the interaction between the amphiphilic copolymers and the lipids, 4,9,22,23,31 and also on the preparation method. Solid-supported polymer and lipid membranes are produced by Langmuir−Blodgett, 32 vesicle fusion, 33,34 in the form of polymer disks, 35 tethered bilayers, 36,37 and recently reported, solvent-assisted (SA) method. 38−40 Of particular interest is the SA method as it is straightforward and requires no special equipment or complex and time-consuming sample preparation conditions.…”

Section: Introductionmentioning

confidence: 99%

“…Solid-supported polymer and lipid membranes are produced by Langmuir–Blodgett, vesicle fusion, , in the form of polymer disks, tethered bilayers, , and recently reported, solvent-assisted (SA) method. − Of particular interest is the SA method as it is straightforward and requires no special equipment or complex and time-consuming sample preparation conditions. , Briefly, the amphiphilic molecules are dissolved in an organic solvent, which is then continuously replaced by an aqueous phase, which triggers the self-assembly process and induces the membrane formation. While the SA method has been previously used to generate lipid membranes , and very recently polymer membranes, its potential to develop hybrid membranes has not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

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Tailoring a Solvent-Assisted Method for Solid-Supported Hybrid Lipid–Polymer Membranes

et al. 2022

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Combining amphiphilic block copolymers and phospholipids opens new opportunities for the preparation of artificial membranes. The chemical versatility and mechanical robustness of polymers together with the fluidity and biocompatibility of lipids afford hybrid membranes with unique properties that are of great interest in the field of bioengineering. Owing to its straightforwardness, the solvent-assisted method (SA) is particularly attractive for obtaining solid-supported membranes. While the SA method was first developed for lipids and very recently extended to amphiphilic block copolymers, its potential to develop hybrid membranes has not yet been explored. Here, we tailor the SA method to prepare solid-supported polymer–lipid hybrid membranes by combining a small library of amphiphilic diblock copolymers poly(dimethyl siloxane)–poly(2-methyl-2-oxazoline) and poly(butylene oxide)- block –poly(glycidol) with phospholipids commonly found in cell membranes including 1,2-dihexadecanoyl- sn -glycero-3-phosphocholine, 1-palmitoyl-2-oleoyl- sn -glycero-3-phosphoethanolamine, sphingomyelin, and 1,2-dioleoyl- sn -glycero-3-phosphoethanolamine- N -(glutaryl). The optimization of the conditions under which the SA method was applied allowed for the formation of hybrid polymer–lipid solid-supported membranes. The real-time formation and morphology of these hybrid membranes were evaluated using a combination of quartz crystal microbalance and atomic force microscopy. Depending on the type of polymer–lipid combination, significant differences in membrane coverage, formation of domains, and quality of membranes were obtained. The use of the SA method for a rapid and controlled formation of solid-supported hybrid membranes provides the basis for developing customized artificial hybrid membranes.

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From spherical compartments to polymer films: exploiting vesicle fusion to generate solid supported thin polymer membranes

Abstract: Solid supported polymer membranes as scaffold for the insertion of functional biomolecules provide the basis for mimicking natural membranes. They also provide the means for unraveling biomolecule-membrane interactions and engineering...

Cited by 8 publications

References 72 publications

Dually Responsive Poly(N-vinylcaprolactam)-b-poly(dimethylsiloxane)-b-poly(N-vinylcaprolactam) Polymersomes for Controlled Delivery

Dually Responsive Poly(N-vinylcaprolactam)-b-poly(dimethylsiloxane)-b-poly(N-vinylcaprolactam) Polymersomes for Controlled Delivery

A DNA‐Micropatterned Surface for Propagating Biomolecular Signals by Positional on‐off Assembly of Catalytic Nanocompartments

Tailoring a Solvent-Assisted Method for Solid-Supported Hybrid Lipid–Polymer Membranes

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