From Starfish Oocytes to Inflammation: The Unforeseeable Destiny of Roscovitine in Cystic Fibrosis

Witko‐Sarsat, Véronique

doi:10.1159/000445967

Cited by 2 publications

(2 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Target neutrophil survival pathways to promote apoptosis of a specific subset to favor the resolution of inflammation could be another option ( 26 ). Consistent with this notion, roscovitine has shown promising results in CF ( 58 , 59 ).…”

Section: Discussionmentioning

confidence: 54%

Specific circulating neutrophils subsets are present in clinically stable adults with cystic fibrosis and are further modulated by pulmonary exacerbations

et al. 2022

Self Cite

View full text Add to dashboard Cite

The progressive lung destruction in cystic fibrosis (CF) is tightly associated with chronic bacterial infection and neutrophil-dominated airway inflammation. CF pulmonary disease is complicated by episodes of acute exacerbations, contributing to irreversible lung damage. We hypothesized that circulating subsets of neutrophils from clinically stable adults with CF present some phenotypic specificities that could amplify their activation during an infectious episode. The aim of the present study was to examine the different neutrophil subsets in whole blood and in the low density neutrophils (LDN) that co-purify with peripheral blood mononuclear cells (PBMC) in clinically stable adults with CF and in CF adults during pulmonary exacerbations compared to healthy donors. Blood samples were obtained from 22 adults with CF (16 in stable state and 6 during pulmonary exacerbations) and from 20 healthy donors. Flow cytometry analysis of 13 different markers related to lineage (CD45, CD15), maturity (CD16, CD10, and CD33), activation (CD62L, CD11b, CD66b, and CD114), metabolism (GLUT-1, LOX1) and immunosuppression (PD1, PD-L1) was carried out within whole blood and within the LDN fraction. Unsupervised analysis of flow cytometry data was performed using visual t-distributed stochastic neighbor embedding (vi-tSNE). A significant increase in the CD11b expression in neutrophils from CF patients during exacerbations was observed compared to neutrophils from stable CF patients or to healthy donors, indicative of a circulating activation state due to an infectious status. The percentage of LDN was not increased in stable CF patients but increased during exacerbations. Analysis of neutrophil subsets using the double CD16/CD62L labeling revealed a significant increase in the CD16high/CD62Llow subset in all CF patients compared to healthy donors. In contrast, an increase in the CD16low/CD62Lhigh subset was observed only in CF patients during exacerbations. Unsupervised analysis identified a PD-L1high/CD114high population that was present in stable CF patients and as well as in CF patients during exacerbations.

show abstract

Section: Discussionmentioning

confidence: 54%

Specific circulating neutrophils subsets are present in clinically stable adults with cystic fibrosis and are further modulated by pulmonary exacerbations

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…The areas that are not covered by our review series, but are of interest for the CF community and discussed in previous publications are: (1) novel anti-inflammatory therapeutic approaches in CF lung disease, such as roscovitine [8,9], antiproteases [10] and others [11], (2) the quantitatively dominant, though functionally controversial role of neutrophils in CF airways, including disturbed apoptosis, neutrophil extracellular trap (NET) formation and other deviations/alterations [12,13,14,15,16,17], (3) impairments of the chemokine system in CF [12,18,19] and (4) the microbiome [3,20,21]. …”

mentioning

confidence: 99%

Innate Immunity in Cystic Fibrosis: Novel Pieces of the Puzzle

Öz

Hartl

2016

J Innate Immun

View full text Add to dashboard Cite

From Starfish Oocytes to Inflammation: The Unforeseeable Destiny of Roscovitine in Cystic Fibrosis

Cited by 2 publications

References 18 publications

Specific circulating neutrophils subsets are present in clinically stable adults with cystic fibrosis and are further modulated by pulmonary exacerbations

Specific circulating neutrophils subsets are present in clinically stable adults with cystic fibrosis and are further modulated by pulmonary exacerbations

Innate Immunity in Cystic Fibrosis: Novel Pieces of the Puzzle

Contact Info

Product

Resources

About