From Tango to Quadrilla

Mazzon, Cristina; Viola, Antonella

doi:10.4161/cam.3982

Cited by 3 publications

(2 citation statements)

References 88 publications

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“…The stability of T cell-DC interaction is determined by multiple interconnected signals, from TCRs as well as stimulatory and inhibitory receptors that are integrated in a specialized membrane junction named the “immunological synapse”-IS ( 32 , 33 ) ( Figure 1 ). In T lymphocytes, signalling events occurring at this platform cause multiple downstream effects ranging from the dynamic rearrangement of the actin cytoskeleton, and the initiation of a gene expression cascade ultimately leading to the generation of effector and memory T cells ( 34 – 36 ).…”

Section: Signalling Compartmentalization In T Cellsmentioning

confidence: 99%

“…Next, we found that the kinase Lck is recruited into CD28-signaling rafts and directed to the IS upon CD28 engagement by a process requiring the CD28 COOH-terminal PxxPP motif and Vav-1, key regulator of the actin cytoskeleton rearrangements ( 63 ). Of note, IS lipid microdomains are also enriched in TCR signalling proteins, including the Src-family kinase Fyn, the adapter protein LAT, phosphoprotein associated with glycosphingolipid-enriched domains (PAG) or Csk-activating protein (Cbp) and Lck-interacting molecule (LIME) ( 33 , 64 ). Interestingly, the partitioning of Lck and LAT at the IS lipid microdomains is dictated by the post-translational modifications (PTM, including protein S-acylation) ( 65 – 67 ).…”

Section: Signalling Compartmentalization In T Cellsmentioning

confidence: 99%

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CD28 and chemokine receptors: Signalling amplifiers at the immunological synapse

2022

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T cells are master regulators of the immune response tuning, among others, B cells, macrophages and NK cells. To exert their functions requiring high sensibility and specificity, T cells need to integrate different stimuli from the surrounding microenvironment. A finely tuned signalling compartmentalization orchestrated in dynamic platforms is an essential requirement for the proper and efficient response of these cells to distinct triggers. During years, several studies have depicted the pivotal role of the cytoskeleton and lipid microdomains in controlling signalling compartmentalization during T cell activation and functions. Here, we discuss mechanisms responsible for signalling amplification and compartmentalization in T cell activation, focusing on the role of CD28, chemokine receptors and the actin cytoskeleton. We also take into account the detrimental effect of mutations carried by distinct signalling proteins giving rise to syndromes characterized by defects in T cell functionality.

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Section: Signalling Compartmentalization In T Cellsmentioning

confidence: 99%

Section: Signalling Compartmentalization In T Cellsmentioning

confidence: 99%

CD28 and chemokine receptors: Signalling amplifiers at the immunological synapse

2022

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La sinapsis inmunológica

Martin

Martín

Sanz

et al. 2013

Medicine - Programa de Formación Médica Continuada Acreditado

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Preparation of CD4<sup>+</sup> T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

Villanueva-Cabello

Martínez-Duncker

2016

JoVE

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The methods described herein for activation of naïve CD4 T cells in suspension and their adherence in coverslips for confocal microscopy analysis allow the spatial localization and visualization of gangliosides involved in CD4 T cell activation, that complement expression profiling experiments such as flow cytometry, western blotting or real-time PCR. The quantification of ganglioside expression through flow cytometry and their cellular localization through microscopy can be obtained by the use of anti-ganglioside antibodies with high affinity and specificity. Nonetheless, an adequate handling of cells in suspension involves the treatment of culture plates to promote the necessary adherence required for fluorescence or confocal microscopy acquisition. In this work, we describe a protocol for determining GD3 and GD2 ganglioside expression and colocalization with the TCR during naïve CD4 T cell activation. Also, real-time PCR experiments using <40,000 cells are described for the determination of the GD3 and GM2/GD2 synthase genes, demonstrating that gene analysis experiments can be performed with a low number of cells and without the need of additional low input RNA kits.

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From Tango to Quadrilla

Cited by 3 publications

References 88 publications

CD28 and chemokine receptors: Signalling amplifiers at the immunological synapse

CD28 and chemokine receptors: Signalling amplifiers at the immunological synapse

La sinapsis inmunológica

Preparation of CD4<sup>+</sup> T Cells for Analysis of GD3 and GD2 Ganglioside Membrane Expression by Microscopy

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