From the lung to the knee joint: Toxicity evaluation of carbon black nanoparticles on macrophages and chondrocytes

Ma, Juan; Guo, Anyi; Wang, Shunhao; Man, Siliang; Zhang, Yunjian; Liu, Sijin; Liu, Yajun

doi:10.1016/j.jhazmat.2018.04.025

Cited by 32 publications

(26 citation statements)

References 54 publications

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“…To the best of our knowledge, the dose- and time-dependent cytotoxicity of GO and rGO has never been tested on human chondrocytes. The cytotoxic analysis on human chondrocytes may assume relevant importance because of the peculiarity of the articular cavities, which are closed spaces along the peripheral circulation route featured by a slow rate interchange of nutrients with the blood [ 25 ]. Using such carbon-based nanomaterials in biodegradable scaffolds for the replacement or regeneration of functions in the cartilage can promote the release of these materials.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes

et al. 2021

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Recently, graphene and its derivatives have been extensively investigated for their interesting properties in many biomedical fields, including tissue engineering and regenerative medicine. Nonetheless, graphene oxide (GO) and reduced GO (rGO) are still under investigation for improving their dispersibility in aqueous solutions and their safety in different cell types. This work explores the interaction of GO and rGO with different polymeric dispersants, such as glycol chitosan (GC), propylene glycol alginate (PGA), and polydopamine (PDA), and their effects on human chondrocytes. GO was synthesized using Hummer’s method, followed by a sonication-assisted liquid-phase exfoliation (LPE) process, drying, and thermal reduction to obtain rGO. The flakes of GO and rGO exhibited an average lateral size of 8.8 ± 4.6 and 18.3 ± 8.5 µm, respectively. Their dispersibility and colloidal stability were investigated in the presence of the polymeric surfactants, resulting in an improvement in the suspension stability in terms of average size and polydispersity index over 1 h, in particular for PDA. Furthermore, cytotoxic effects induced by coated and uncoated GO and rGO on human chondrocytes at different concentrations (12.5, 25, 50 and 100 µg/mL) were assessed through LDH assay. Results showed a concentration-dependent response, and the presence of PGA contributed to statistically decreasing the difference in the LDH activity with respect to the control. These results open the way to a potentially safer use of these nanomaterials in the fields of cartilage tissue engineering and regenerative medicine.

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Section: Introductionmentioning

confidence: 99%

Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes

et al. 2021

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“…The biocompatibility is a crucial assessment for biomaterials [ 5 , 38 , 39 ]. To assess the cytotoxicity of the composite GMO hydrogels in vitro , BMSCs and HUVECs were used for the subsequent experiments.…”

Section: Resultsmentioning

confidence: 99%

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Zhou

Luo

et al. 2022

Bioactive Materials

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“…Studies have shown that CBNPs have obvious toxic effects on animals. For example, after CBNPs invade organisms, they can cause oxidative stress in the organism, affect the structure and function of lysozyme, and also cause lung and systemic in ammation (Ma et al 2018, Zhang et al 2019a. At the same time, CBNPs have also been proved to have signi cant effects on the liver.…”

Section: Introductionmentioning

confidence: 99%

Carbon Black Nanoparticles Exposure Induces Intestinal Flora Dysbiosis and Consequent Activation of Gut-liver Axis Leading to Liver Lipid Accumulation in Zebrafish

Liu

Wang

et al. 2021

Preprint

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BackgroundCarbon black nanoparticles (CBNPs) are a major carbonaceous nanomaterial, which have been widely left in the environment. The integrity of the gut-liver axis function is critical to the survival of animals. Therefore, we studied the effects of three concentrations of CBNPs (50, 100, 200 mg/L) on zebrafish intestines, liver and intestinal flora. ResultsThe results showed that CBNPs exposure could reduce the diversity of intestinal flora, change the structure of core microbial populations, enhance the permeability of the intestinal mucosal barrier, and cause changes in genes related to tight junctions in intestinal tissues. The H&E staining and Oil red O staining showed that CBNPs exposure would lead to vacuolar degeneration and lipid accumulation in zebrafish liver. Further detection of glycolipid metabolism related genes showed that CBNPs exposure induced the up-regulation of glycolysis related genes PFKFB3, LDHA, and LEPr, reduces the expression of glycogen synthase kinase GSK-3b, and increases lipid transport and production related genes PPAR-α, PPAR-γ, LIPC, apoa4, Fabp2 and Fabp11 expression. ConclusionsIn brief, our data demonstrated that CBNPs exposure induced intestinal microflora disturbance in zebrafish can lead to liver lipid accumulation.

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From the lung to the knee joint: Toxicity evaluation of carbon black nanoparticles on macrophages and chondrocytes

Cited by 32 publications

References 54 publications

Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes

Graphene Oxide and Reduced Graphene Oxide Nanoflakes Coated with Glycol Chitosan, Propylene Glycol Alginate, and Polydopamine: Characterization and Cytotoxicity in Human Chondrocytes

Synergistic osteogenic and angiogenic effects of KP and QK peptides incorporated with an injectable and self-healing hydrogel for efficient bone regeneration

Carbon Black Nanoparticles Exposure Induces Intestinal Flora Dysbiosis and Consequent Activation of Gut-liver Axis Leading to Liver Lipid Accumulation in Zebrafish

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