From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

Khan, Raheema L.; Khraibi, Ali A.; Dumée, Ludovic F.; Corridon, Peter R.

doi:10.3389/fbioe.2023.1091554

Cited by 26 publications

(19 citation statements)

References 182 publications

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“…This characteristic supports its conduciveness xenografting [25]. Moreover, recent studies have also revealed the potential to generate a limitless supply of corneal xenografts using slaughterhouse waste [11,[27][28][29][30]. Based on this potential, we assess current applications and performance in preclinical and clinical practices of various decellularization protocols used to create Kpros and their ability to maintain pertinent structural and physiological capacities.…”

Section: Introductionmentioning

confidence: 79%

“…It provides a more effective alternative to ease the constraints of autologous grafting than synthetic vascular tissue engineering procedures [10,20]. The elimination of cellular components and related waste should reduce any potential host rejection or immune reaction [27]. The purpose of corneal decellularization is to yield biocompatible ECM components that possess latent biochemical cues to support in vivo tissue remodeling and long-term transplantability [10,20].…”

Section: Methods Of Corneal Decellularizationmentioning

confidence: 99%

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A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

Wang¹,

Elbahrawi²,

Abdukadir³

et al. 2023

Preprint

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Corneal opacity is a leading cause of vision impairment and suffering worldwide. Transplantation can effectively restore vision and reduce chronic discomfort. However, there is a considerable shortage of viable corneal graft tissues. Tissue engineering may address this issue by advancing xeno- keratoplasty as a viable alternative to conventional keratoplasty. In particular, livestock decellularization strategies offer the potential to generate bioartificial ocular prosthetics in sufficient supply to match existing and projected needs. To this end, we have examined the best practices and characterizations that have supported the current state-of-the-art driving preclinical and clinical applications. Identifying the challenges that delimit activities to supplement the donor corneal pool derived from acellular scaffolds allowed us to hypothesize a model for keratoprosthesis applications derived from livestock combining 3D printing and decellularization.

show abstract

Section: Introductionmentioning

confidence: 79%

Section: Methods Of Corneal Decellularizationmentioning

confidence: 99%

A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

Wang¹,

Elbahrawi²,

Abdukadir³

et al. 2023

Preprint

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show abstract

“…Furthermore, functional cross-linking techniques may generate interconnected fibrillar scaffolds that can support tunable toughness and strength 80 . Likewise, additional coatings can also be employed to reduce scaffold antigenicity 10 and porosity 81 . Again, such coating molecules can be coupled with fluorescent labels for future IVM investigations to gauge their effectiveness in supporting optimized approaches.…”

Section: Discussionmentioning

confidence: 99%

“…Whole organ decellularization has been described as one of the most promising ways to construct a bioartificial kidney 6 . Decellularization focuses on extracting the extracellular matrix (ECM) from the native tissue and organ with as many structural and functional clues as possible 7 – 10 . The ECM can then be employed as a natural template for regeneration, as observed traditionally in commercial substitutes 11 – 13 .…”

Section: Introductionmentioning

confidence: 99%

Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy

Corridon

2023

Sci Rep

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Organ decellularization creates cell-free, collagen-based extracellular matrices that can be used as scaffolds for tissue engineering applications. This technique has recently gained much attention, yet adequate scaffold repopulation and implantation remain a challenge. Specifically, there still needs to be a greater understanding of scaffold responses post-transplantation and ways we can improve scaffold durability to withstand the in vivo environment. Recent studies have outlined vascular events that limit organ decellularization/recellularization scaffold viability for long-term transplantation. However, these insights have relied on in vitro/in vivo approaches that need enhanced spatial and temporal resolutions to investigate such issues at the microvascular level. This study uses intravital microscopy to gain instant feedback on their structure, function, and deformation dynamics. Thus, the objective of this study was to capture the effects of in vivo blood flow on the decellularized glomerulus, peritubular capillaries, and tubules after autologous and allogeneic orthotopic transplantation into rats. Large molecular weight dextran molecules labeled the vasculature. They revealed substantial degrees of translocation from glomerular and peritubular capillary tracks to the decellularized tubular epithelium and lumen as early as 12 h after transplantation, providing real-time evidence of the increases in microvascular permeability. Macromolecular extravasation persisted for a week, during which the decellularized microarchitecture was significantly and comparably compromised and thrombosed in both autologous and allogeneic approaches. These results indicate that in vivo multiphoton microscopy is a powerful approach for studying scaffold viability and identifying ways to promote scaffold longevity and vasculogenesis in bioartificial organs.

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“…A greater understanding of the roles of key signaling pathways has also allowed investigators to differentiate stem cell niches into various lineages. We believe that shortly, organoids derived from patients’ cells will be able to repopulate decellularized renal scaffolds and printed tissues or even be injected back into the patients to restore their native dysfunction ( 69 – 71 ). Nevertheless, many technical ( 72 – 78 ) and ethical ( 79 – 87 ) issues still need to be solved in this field.…”

Section: Efforts To Devise Effective Genetic Alterations In the Kidneymentioning

confidence: 99%

Still finding ways to augment the existing management of acute and chronic kidney diseases with targeted gene and cell therapies: Opportunities and hurdles

Corridon

2023

Front. Med.

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The rising global incidence of acute and chronic kidney diseases has increased the demand for renal replacement therapy. This issue, compounded with the limited availability of viable kidneys for transplantation, has propelled the search for alternative strategies to address the growing health and economic burdens associated with these conditions. In the search for such alternatives, significant efforts have been devised to augment the current and primarily supportive management of renal injury with novel regenerative strategies. For example, gene- and cell-based approaches that utilize recombinant peptides/proteins, gene, cell, organoid, and RNAi technologies have shown promising outcomes primarily in experimental models. Supporting research has also been conducted to improve our understanding of the critical aspects that facilitate the development of efficient gene- and cell-based techniques that the complex structure of the kidney has traditionally limited. This manuscript is intended to communicate efforts that have driven the development of such therapies by identifying the vectors and delivery routes needed to drive exogenous transgene incorporation that may support the treatment of acute and chronic kidney diseases.

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From waste to wealth: Repurposing slaughterhouse waste for xenotransplantation

Cited by 26 publications

References 182 publications

A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

A Proposed Model of Xeno-Keratoplasty Using 3D Printing and Decellularization

Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy

Still finding ways to augment the existing management of acute and chronic kidney diseases with targeted gene and cell therapies: Opportunities and hurdles

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