Frozen section analysis of sentinel lymph nodes in melanoma patients

Koopal, Sietze A.; Tiebosch, A.T.M.G.; Piers, D. A.; Plukker, John; Koops, Heimen Schraffordt; Hoekstra, Harald J.

doi:10.1002/1097-0142(20001015)89:8<1720::aid-cncr11>3.0.co;2-f

Cited by 62 publications

(22 citation statements)

References 21 publications

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“…In breast cancer, reports on intraoperative SLN sensitivity range from 47% to 74% (22,23) whereas in melanoma the sensitivity is even worse, with reports from 38% to 47% (24,25). Consequently, many patients have to undergo a second surgical procedure to complete lymph node dissection after definitive pathologic assessment identifies microscopic metastatic disease not evident on intraoperative analysis.…”

Section: Introductionmentioning

confidence: 99%

Molecular Staging of Cervical Lymph Nodes in Squamous Cell Carcinoma of the Head and Neck

Ferris

Raja³

et al. 2005

Cancer Research

103

119

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Clinical staging of cervical lymph nodes from patients with squamous cell carcinoma of the head and neck (SCCHN) has only 50% accuracy compared with definitive pathologic assessment. Consequently, both clinically positive and clinically negative patients frequently undergo neck dissections that may not be necessary. To address this potential overtreatment, sentinel lymph node (SLN) biopsy is currently being evaluated to provide better staging of the neck. However, to fully realize the potential improvement in patient care afforded by the SLN procedure, a rapid and accurate SLN analysis is necessary. We used quantitative reverse transcription--PCR (QRT-PCR) to screen 40 potential markers for their ability to detect SCCHN metastases to cervical lymph nodes. Seven markers were identified with good characteristics for identifying metastatic disease, and these were validated using a set of 26 primary tumors, 19 histologically positive lymph nodes, and 21 benign nodes from patients without cancer. Four markers discriminated between positive and benign nodes with accuracy > >97% but only one marker, pemphigus vulgaris antigen (PVA), discriminated with 100% accuracy in both the observed data and a statistical bootstrap analysis. A rapid QRT-PCR assay for PVA was then developed and incorporated into a prototype instrument capable of performing fully automated RNA isolation and QRT-PCR. The automated analysis with PVA provided perfect discrimination between histologically positive and benign lymph nodes and correctly identified two lymph nodes with micrometastatic tumor deposits. These assays were completed ( from tissue to result) in f f30 minutes, thus demonstrating the feasibility of intraoperative staging of SCCHN SLNs by QRT-PCR. (Cancer Res 2005; 65(6): 2147-56)

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Section: Introductionmentioning

confidence: 99%

Molecular Staging of Cervical Lymph Nodes in Squamous Cell Carcinoma of the Head and Neck

Ferris

Raja³

et al. 2005

Cancer Research

103

119

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“…Additional positive nonSLNs were identified in 10 of 74 patients (14%). This low sensitivity was confirmed in other subsequent studies (Koopal et al 2000;Tanis et al 2001). The authors do suggest the potential use of frozen sectioning of a sentinel node found within the parotid gland or neck, thereby reducing the risk of facial and spinal accessory nerve injury during a second operative procedure.…”

Section: Other Emerging Strategiesmentioning

confidence: 54%

Impact of Sentinel Node Biopsy on Outcome in Melanoma

Kelly¹,

Kerin²

2011

Treatment of Metastatic Melanoma

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“…The difficulty in finding small deposits of tumor cells in lymph nodes is an important disadvantage of this technique and its indication is controversial [12][13][14] .…”

Section: Discussionmentioning

confidence: 99%

Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis

et al. 2014

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PURPOSE:To simulate a lymph node metastasis in an animal model using activated carbon, assess their identification in frozen section analysis and compare with histopathological examination in paraffin. METHODS:Thirty two adult female rats were used. They received the carbon injection on its hind legs. Half of the rats was sacrificed on day one, and the other half after 21 days. Thus, 64 lymph nodes were dissected and split longitudinally. One half of the lymph node was sent immediately to frozen section analysis. The other half was fixed in 10% formaldehyde to be cut in paraffin. Slides were divided into quadrants and classified by the presence of carbon in these four quadrants_ They were also classified by the carbon staining intensity. RESULTS:Comparing the slides obtained in the first day and 21 days, there was a tendency of carbon to spread over time, but without statistical significance. The intensity did not alter over time. CONCLUSION:There was no concordance between the two methods of pathological analysis, however the actived carbon was seen in all lymph nodes.

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Frozen section analysis of sentinel lymph nodes in melanoma patients

Cited by 62 publications

References 21 publications

Molecular Staging of Cervical Lymph Nodes in Squamous Cell Carcinoma of the Head and Neck

Molecular Staging of Cervical Lymph Nodes in Squamous Cell Carcinoma of the Head and Neck

Impact of Sentinel Node Biopsy on Outcome in Melanoma

Sentinel lymph node biopsy in rats. Comparison between paraffin and frozen section analysis

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