Fructan accumulation induced by nitrogen deficiency in barley leaves correlates with the level of sucrose:fructan 6-fructosyltransferase mRNA

Wang, Changwen; Ende, Wim Van den; Tillberg, Jan‐Eric

doi:10.1007/s004250000335

Cited by 51 publications

(50 citation statements)

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“…Therefore, although SST and FFT activities were not evaluated in the present study, the higher fructan concentration found in N-limited plants could be associated with increases in the activity of these enzymes, as reported by Wang et al (2000) for 6-SFT in barley leaves. Since sucrose is the precursor molecule in the biosynthesis of fructans, the metabolism of these two compounds is closely related.…”

Section: Discussionmentioning

confidence: 66%

Growth, photosynthate partitioning and fructan accumulation in plants of Vernonia herbacea (Vell.) Rusby under two nitrogen levels

Cuzzuol

Carvalho

Zaidan

2005

Braz. J. Plant Physiol.

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The effect of two nitrogen concentrations on fructan accumulation and plant growth was analysed in plants of Vernonia herbacea during a year period. Plants of this species accumulate inulin-type fructans in the underground reserve organs (rhizophores). The plants were cultivated in glasshouse conditions and received weekly nutrient solutions containing 1.3 mmol.L -1 NO 3 -(Nlimited) or 10.7 mmol.L -1 NO 3 -(N-sufficient). Plants treated with N-sufficient solution presented an increase in total growth with higher biomass, more but smaller leaves resulting in higher total leaf area, higher net assimilation rate, specific leaf mass and higher biomass allocation to aerial organs. In addition, these plants presented higher contents of reducing sugars and lower fructan contents. In contrast, N-limited plants showed reduced total growth with lower biomass, higher biomass allocation to underground organs and higher fructan contents. Therefore, although N-limited plants presented a higher fructan concentration, the amount of fructan produced per plant was similar in both N treatments. Key words: Asteraceae, cerrado, fructans, underground reserve organs.Crescimento, partição de fotossintatos e acúmulo de frutanos em plantas de Vernonia herbacea (Vell.) Rusby tratadas com dois níveis de nitrogênio. O efeito de duas concentrações de nitrogênio sobre o acúmulo de frutanos e seu crescimento foi analisado durante um ano em plantas de Vernonia herbacea. As plantas foram cultivadas em casa de vegetação e receberam semanalmente solução nutritiva contendo 1,3 mmol.L -1 NO 3 -(N-limitado) ou 10,7 mmol.L -1 NO 3 -(N-suficiente). As plantas tratadas com a solução N-suficiente apresentaram maior crescimento, com maior biomassa, número maior de folhas, porém com menor área, resultando em área foliar total mais elevada, taxa de assimilação líquida e massa foliar específica mais altas, maior alocação de biomassa para os órgãos aéreos. Além disso, revelaram teores mais altos de açúcares redutores e mais baixos de frutanos. Já as plantas que receberam solução N-limitada apresentaram menor crescimento com menor biomassa, maior alocação de biomassa para os órgãos subterrâneos e maior conteúdo de frutanos. Portanto, embora as plantas sob Nlimitado tenham apresentado maior concentração de frutanos, sua produção por planta foi semelhante nos dois tratamentos de nitrogênio. Palavras-chave: Asteraceae, cerrado, frutanos, órgãos subterrâneos de reserva.

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Section: Discussionmentioning

confidence: 66%

Growth, photosynthate partitioning and fructan accumulation in plants of Vernonia herbacea (Vell.) Rusby under two nitrogen levels

Cuzzuol

Carvalho

Zaidan

2005

Braz. J. Plant Physiol.

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“…Fructan biosynthesis is initiated by the enzyme sucrose:sucrose 1-fructosyltransferase (1-SST), which catalyses the formation of 1-kestose from sucrose (Vijn et al, 1998). It has been reported that 1-SST was induced by high sucrose contents in barley leaves (Muller et al, 2000;Wang et al, 2000). These indicate that the enzyme genes which catalyze sucrose biosynthesis are also important for fructan productions.…”

Section: Discussionmentioning

confidence: 99%

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

et al. 2009

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To develop the bunching onion (Allium fistulosum L.; genomes, FF) chromosomespecific genetic markers for identifying extra chromosomes, eight shallot (A. cepa L. Aggregatum group; genomes, AA) -A. fistulosum monosomic addition plants (AA+nF) and 62 shallot -A. fistulosum single-alien deletion plants (AAF-nF) were analyzed by 23 different chromosome-specific genetic markers of shallot. The eight monosomic addition plants consisted of one AA+2F, two AA+6F, and five AA+8F. Of the 62 single-alien deletion plants, 60 could be identified as six different single-alien deletion lines (AAF-1F, -3F, -4F, -6F, -7F, and -8F) out of the eight possible types. Several single-alien deletion lines were classified on the basis of leaf and bulb characteristics. AAF-8F had the largest number of expanded leaves of five deletion plants. AAF-7F grew most vigorously, as expressed by its long leaf blade and biggest bulb size. AAF-4F had very small bulbs. AAF-7F and AAF-8F had different bulbs from those of shallot as well as other types of single-alien deletion lines in skin and outer scale color. Regarding the sugar content of the bulb tissues, the single-alien deletion lines showed higher fructan content than shallot. Moreover, shallot could not produce fructan with degree of polymerization (DP) 12 or higher, although the single-alien deletion lines showed DP 20 or higher. The content of S-alk(en)yl-L-cysteine sulfoxide (ACSO) in the single-alien deletion lines was significantly lower than that in shallot. These results indicated that chromosomes from A. fistulosum might carry anonymous factors to increase the highly polymerized fructan production and inhibit the synthesis of ACSO in shallot bulbs. Accordingly, alien chromosomes from A. fistulosum in shallot would contribute to modify the quality of shallot bulbs.

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“…(Amiard et al, 2003;Wang et al, 2003). A detailed fructan analysis in both Clipper and Sahara leaves may clarify whether these compounds have any direct involvement in B tolerance.…”

Section: Metabolite Profiling Of Barley In Response Tomentioning

confidence: 99%

An Investigation of Boron Toxicity in Barley Using Metabolomics

et al. 2006

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Boron (B) is an essential micronutrient that affects plant growth at either deficient or toxic concentrations in soil. The aim of this work was to investigate the adaptation of barley (Hordeum vulgare) plants to toxic B levels and to increase our understanding of B toxicity tolerance mechanisms. We used a metabolomics approach to compare metabolite profiles in root and leaf tissues of an intolerant, commercial cultivar (cv Clipper) and a B-tolerant Algerian landrace (cv Sahara). After exposure to elevated B (200 and 1,000 mM), the number and amplitude of metabolite changes in roots was greater in Clipper than in Sahara. In contrast, leaf metabolites of both cultivars only responded following 1,000 mM treatment, at which B toxicity symptoms (necrosis) were visible. In addition, metabolite levels were dramatically altered in the tips of leaves of the sensitive cultivar Clipper after growth in 1,000 mM B compared to those of Sahara. This correlates with a gradual accumulation of B from leaf base to tip in B-intolerant cultivars. Overall, there were always greater differences between tissue types (roots and leaves) than between the two cultivars. This work has provided insights into metabolic differences of two genetically distinct barley cultivars and information about how they respond metabolically to increasing B levels.Boron (B) is an essential micronutrient for vascular plants. However, when B is present at high concentrations in the soil or ground water, plant growth and reproduction can be affected by B toxicity. B toxicity has been recognized as an important problem limiting crop production in the low rainfall and on highly alkaline and saline soils in regions of Australia, West Asia, and North Africa. Because soil amelioration is impractical, the development of B-tolerant cultivars is a rational solution to the problem.B freely diffuses into the roots as boric acid [B(OH) 3 ; pK a 5 9.25] and accumulates in the cytoplasm as the borate anion [B(OH) 4 2 ] due to pH-dependent interconversion. An inability to exclude B from the roots results in high B concentrations in the tissue. B phytotoxicity manifests itself in a broad range of physiological effects, including decreased shoot and root growth, root cell division and RNA content, reduced leaf chlorophyll, lower photosynthetic rates and stomatal conductance, and reduced levels of lignin and suberin (for review, see Nable et al., 1997). Leaf symptoms of toxicity in barley (Hordeum vulgare) are characterized by interveinal chlorotic and/or necrotic patches, generally at the margins and tips of older leaves. This reflects the accumulation of B at the end of the transpiration stream (Nable et al., 1997). Following long-term exposure to high B concentrations in the soil, overall vegetative plant growth is retarded and this leads to either a reduction in or a complete lack of seed set.B is also an essential nutrient, although its role in plant growth, development, and metabolism remains to be clarified. Originally, B was thought to be essentially immobile in the ...

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Fructan accumulation induced by nitrogen deficiency in barley leaves correlates with the level of sucrose:fructan 6-fructosyltransferase mRNA

Cited by 51 publications

References 31 publications

Growth, photosynthate partitioning and fructan accumulation in plants of Vernonia herbacea (Vell.) Rusby under two nitrogen levels

Growth, photosynthate partitioning and fructan accumulation in plants of Vernonia herbacea (Vell.) Rusby under two nitrogen levels

Molecular and biochemical identification of alien chromosome additions in shallot (Allium cepa L. Aggregatum group) carrying extra chromosome(s) of bunching onion (A. fistulosum L.)

An Investigation of Boron Toxicity in Barley Using Metabolomics

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