Fructose-responsive genes in the small intestine of neonatal rats

Cui, Xuelin; Soteropoulos, Patricia; Tolias, Peter; Ferraris, Ronaldo P.

doi:10.1152/physiolgenomics.00056.2004

Cited by 48 publications

(48 citation statements)

References 40 publications

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“…Furthermore, it is known that glucocorticoid hormones (Si, Slc2a5 and Treh) and thyroid hormones (Si, Slc2a5 and Treh) enhance intestinal gene expressions during the transient suckling-weaning period (4,5,13,32). In addition, the expressions of these genes are known to be upregulated by dietary carbohydrate (9,10,51,52). Therefore, the changes in gene expression between days 13 and 27 detected in our microarray analysis may be regulated by these hormones and dietary factors.…”

Section: Discussionmentioning

confidence: 74%

“…Agbemafle et al ( 8 ) examined glucocorticoid hormoneresponsive genes during the suckling period by microarray analysis. Cui et al ( 9,10 ) showed that fructoseresponsive genes, such as glucose-6-phosphatase, fructose-1, 6-bisphosphatase and the PI3 kinase cascade, were upregulated by fructose perfusion in weaning rats. However, these observations only focused on glucocorticoid hormone-or fructose-responsive genes, and the detailed changes in gene expression in the rat small intestine during the transient suckling-weaning period remain unknown.…”

Section: Discussionmentioning

confidence: 99%

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Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Mochizuki

Yorita

Goda

2009

J Nutr Sci Vitaminol

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SummaryIt is well-known that the small intestine of rodents rapidly undergoes differentiation and maturation during the transient suckling-weaning period from postnatal days 13 to 27. In the present study, we examined the gene expression changes in the jejunum of rats during the transient suckling-weaning period by microarray analysis. In the microarray data, we found that the expressions of many genes related to digestion/absorption/ excretion of nutrients/ions, such as members of the solute carrier ( Slc ) family and ATP-binding cassette ( Abc ) subfamily, were rapidly induced during this period. Furthermore, some transcriptional factors/cofactors ( Thrsp , Ppargc1a , Klf15 and Vdr ), which are presumably important for the induction of intestinal gene expression after weaning, were rapidly induced during this period. In contrast, genes related to transport of nutrients, such as folate, zinc, fat and phosphate, which are important for early development, were highly expressed in the suckling period and then gradually decreased during weaning. These results indicate that the jejunum matures during the suckling-weaning period accompanied by changes in the expression of many genes related to digestion/absorption/excretion and some genes for transcriptional factors/cofactors. Key Words transient suckling-weaning period, jejunum, solute carrier family, ATP-binding cassette subfamily, transcriptional factors/cofactorsThe morphology of the small intestine in rodents changes dramatically during the first 2-3 wk after birth as they are gradually weaned. During this period, the diet composition changes from one with less carbohydrate (milk) to one rich in carbohydrate (solid food) ( 1 ). Several studies have already shown that the expressions of genes related to carbohydrate digestion/absorption, such as disaccharidases [sucrase-isomaltase ( Si ) and trehalase ( Treh )], which are involved in the digestion of starch/sucrose or trehalose into monosaccharides, and hexose transporters [SGLT1 ( Slc5a1 ), GLUT5 ( Slc2a5 ) and GLUT2 ( Slc2a2 )], which are involved in monosaccharide absorption from the lumen, are elevated between 2 and 3 wk after birth in rodents ( 2 -5 ). In addition, we previously reported that the gene expressions of liver-type fatty acid-binding protein [L-FABP ( Fabp1 )] and cellular retinol-binding protein type II [CRBPII ( Rbp2 )], which are involved in transporting fatty acids and vitamin A, respectively, from the lumen to enterocytes, as well as a ␤ -oxidation rate-controlling gene [acyl-CoA oxidase ( Acox1 )], were highly expressed in the transient suckling-weaning period, and declined after weaning ( 6 , 7 ). Furthermore, the gene expression of peroxisome proliferator-activated receptor ␣ [PPAR ␣ ( Ppara )], a transcriptional factor for these genes, was associated with these gene expression changes ( 6 ).These changes during the transient suckling-weaning period may be regulated by the nutritional changes as well as hormones, such as thyroid hormone and glucocorticoid hormone, because such hormones ar...

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Mochizuki

Yorita

Goda

2009

J Nutr Sci Vitaminol

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“…to stimulate phosphofructo-2-kinase/fructose-2,6-bisphosphatase transcription in neonatal rat intestine (9).…”

Section: Discussionmentioning

confidence: 99%

Fructose-induced increases in neonatal rat intestinal fructose transport involve the PI3-kinase/Akt signaling pathway

Cui¹,

Schlesier²,

Fisher³

et al. 2005

American Journal of Physiology-Gastrointestinal and Liver Physi

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Cui, Xue-Lin, Anna M. Schlesier, Elda L. Fisher, Carla Cerqueira, and Ronaldo P. Ferraris. Fructose-induced increases in neonatal rat intestinal fructose transport involve the PI3-kinase/Akt signaling pathway. Am J Physiol Gastrointest Liver Physiol 288: G1310 -G1320, 2005. First published February 3, 2005 doi:10.1152/ajpgi.00550.2004.-Expression of rat glucose transporter-5 (GLUT5) is tightly regulated during development. Expression and activity are low throughout the suckling and weaning stages, but perfusion of the small intestinal lumen with fructose solutions during weaning precociously enhances GLUT5 activity and expression. Little is known, however, about the signal transduction pathways involved in the substrate-induced precocious GLUT5 development. We found that wortmannin and LY-294002, inhibitors of phosphatidylinositol 3-kinase (PI3-kinase) specifically inhibited the increase in fructose uptake rate and brushborder GLUT5 protein abundance but not GLUT5 mRNA abundance. Perfusion of EGF, an activator of PI3-kinase, also resulted in a marked wortmannin-inhibitable increase in fructose uptake. Perfusion of fructose for 4 h increased cytosolic immunostaining of phosphatidylinositol-3,4,5-triphosphate (PIP 3), the primary product of PI3-kinase, mainly in the mid-to upper-villus regions in which the brush-border membrane also stained strongly with GLUT5. Perfusion of glucose for 4 h had little effect on fructose or glucose uptake and PIP 3 or GLUT5 staining. SH-5, an Akt inhibitor, prevented the increase in fructose uptake and GLUT5 protein induced by fructose solutions, and had no effect on glucose uptake. The PI3-kinase/Akt signaling pathway may be involved in the synthesis and/or recruitment to the brush border of GLUT5 transporters by luminal fructose in the small intestine of weaning rats. Increases in fructose transport during the critical weaning period when rats are shifting to a new diet may be modulated by several signaling pathways whose cross talk during development still needs to be elucidated. development; glucose; intestine; epidermal growth factor; mucosa BECAUSE OF DRAMATIC INCREASES in consumption of soft drinks and fruit juices, per capita consumption of fructose in the United States has increased by 10 times in 30 yr to almost 60 g fructose per day (4, 39). Paralleling this remarkable increase in fructose consumption is an alarming increase in incidence of obesity and in prevalence of type II diabetes (4,14). What makes this correlation disturbing is that per capita fructose consumption in very young children has increased faster than that of the general population and in the 1980s was already ϳ30 -40 g fructose per day representing 10% of their energy intake (39). The top 10th percentile of subjects in all age groups typically consume approximately two times more fructose, exposing this particular age group (1-6 yr of age) to potential metabolic derangements caused by excessive fructose consumption. There are very few studies on physiological adaptations to excessive fructose consumpti...

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“…In suckling rats younger than 14 days, gavage feeding or perfusion in vivo of fructose has no effect on the already low levels of GLUT5 expression and activity ( Fig. 1) (38,45,74). This is understandable since milk is fructose free and there is no luminal signal that can stimulate GLUT5.…”

Section: Physiology and Functionmentioning

confidence: 95%

Regulation of the fructose transporter GLUT5 in health and disease

Douard¹,

Ferraris²

2008

American Journal of Physiology-Endocrinology and Metabolism

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400

366

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Fructose is now such an important component of human diets that increasing attention is being focused on the fructose transporter GLUT5. In this review, we describe the regulation of GLUT5 not only in the intestine and testis, where it was first discovered, but also in the kidney, skeletal muscle, fat tissue, and brain where increasing numbers of cell types have been found to have GLUT5. GLUT5 expression levels and fructose uptake rates are also significantly affected by diabetes, hypertension, obesity, and inflammation and seem to be induced during carcinogenesis, particularly in the mammary glands. We end by highlighting research areas that should yield information needed to better understand the role of GLUT5 during normal development, metabolic disturbances, and cancer.

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Fructose-responsive genes in the small intestine of neonatal rats

Cited by 48 publications

References 40 publications

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Gene Expression Changes in the Jejunum of Rats during the Transient Suckling-Weaning Period

Fructose-induced increases in neonatal rat intestinal fructose transport involve the PI3-kinase/Akt signaling pathway

Regulation of the fructose transporter GLUT5 in health and disease

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