FT-IR Microspectroscopy on molecular building of Zebrafish oocytes

Carnevali, Oliana; Conti, Carla; Ferraris, P.; Garavaglia, Maria Grazia; Gioacchini, Giorgia; Giorgini, Elisabetta; Rubini, Corrado; Sabbatini, Simona; Tosi, Giorgio

doi:10.1016/j.molstruc.2009.09.029

Cited by 27 publications

(14 citation statements)

References 23 publications

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“…Three peaks were observed at 3439, 2925, and 2849 cm À1 at 2500-3500 cm À1 and assigned to the -OH mode and CH 2 and C-H bands, respectively. [35][36][37] In addition, the signals at 1634, 1448, 1388, and 1055 cm À1 can be assigned to the CQC groups, CQO band, COO-groups, and C-O band, respectively. 38 To observe the morphology of the as-synthesized samples, we conducted FESEM (Fig.…”

Section: Resultsmentioning

confidence: 99%

V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

Xiao

Zhang

Xia

et al. 2019

Mater. Chem. Front.

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Section: Resultsmentioning

confidence: 99%

V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

Xiao

Zhang

Xia

et al. 2019

Mater. Chem. Front.

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“…Additional studies using different methodological approach such as the Fourier Transform Infrared (FTIR) microspectroscopy, a powerful method to study the composition and the macromolecular chemistry of fish oocytes [64] , [65] are actually in progress to provide e a biochemical fingerprint of oocytes from females exposed to melatonin to better understand the role of this hormone on zebrafish reproduction.…”

Section: Discussionmentioning

confidence: 99%

Melatonin Induces Follicle Maturation in Danio rerio

et al. 2011

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Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin by the pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin. Melatonin led to an increase of the Gonado Somatic Index (GSI) associated with the increase of eggs production, and the raise of gene and protein levels of vitellogenin (VTG) and estradiol receptor α (ERα) in the liver. The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15. The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation. Melatonin also exerted a direct action on follicles as shown by the increase of the oocytes undergoing to germinal vesicle break down (GVBD) and modulated mpr α and β gene expression in the in vitro exposure.These data highlight the effects of melatonin in promoting zebrafish reproduction exerting its effects either in the brain-pituitary and in the gonads.

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“…By coupling IR spectrometers with bidimensional arrays detectors, it is possible to spectroscopically map specific areas of non-homogeneous biological samples, providing, at the same time and on the same sample, unique biochemical and ultrastructural information 7,8 . In recent years, several reports exploited FTIRI to characterize the biochemical changes associated with oocyte growth and maturation, in various species including fish 9–13 . In addition, recently, this spectroscopic tool has been also applied to evaluate the macromolecular alterations induced in zebrafish by feed additives and pollutants 14–16 .…”

Section: Introductionmentioning

confidence: 99%

Macromolecular Characterization of Swordfish Oocytes by FTIR Imaging Spectroscopy

Carnevali

Candelma

Sagrati

et al. 2019

Sci Rep

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During folliculogenesis, primary oocytes of teleosts grow by several orders of magnitude by-self synthesizing proteins and mRNA, or sequestering from blood specific macromolecular components, such as fatty acids and vitellogenin. All these materials are stored into cortical alveoli, yolk globules or oil droplets during oocyte development. The proper synthesis, storage and displacement of these macromolecular components inside the oocyte play a key role for a successful fertilization process and for the subsequently correct embryo development. In this study, for the first time, the FTIR Imaging (FTIRI) spectroscopy has been applied to characterize the chemical building blocks of several cellular components of swordfish oocytes at different developmental stages. In particular, the spectral features of previtellogenic (PV), vitellogenic (VTG), mature (M) and atretic (A) follicles as well as and of cortical alveoli (CA), yolk vesicles (YV), oil droplets (OD) and Zona Radiata (ZR) have been outlined, providing new insights in terms of composition and topographical distribution of macromolecules of biological interest such as lipids, proteins, carbohydrates and phosphates. The macromolecular characterization of swordfish oocytes at different developmental stages represents a starting point and a useful tool for the assessment of swordfish egg quality caught in different conditions, such as periods of the year or different fishing area.

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FT-IR Microspectroscopy on molecular building of Zebrafish oocytes

Cited by 27 publications

References 23 publications

V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

Melatonin Induces Follicle Maturation in Danio rerio

Macromolecular Characterization of Swordfish Oocytes by FTIR Imaging Spectroscopy

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FT-IR Microspectroscopy on molecular building of Zebrafish oocytes

Cited by 27 publications

References 23 publications

V2(PO4)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

V2(PO4)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

Melatonin Induces Follicle Maturation in Danio rerio

Macromolecular Characterization of Swordfish Oocytes by FTIR Imaging Spectroscopy

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Product

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V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries

V₂(PO₄)O/C@CNT hollow spheres with a core–shell structure as a high performance anode material for lithium-ion batteries