FTIR and WAXD Study of Regenerated Silk Fibroin

Kamalha, Edwin; Zheng, Yuan Sheng; Zeng, Yongchun; Fredrick, Mutua N.

doi:10.4028/www.scientific.net/amr.677.211

Cited by 32 publications

(33 citation statements)

References 19 publications

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“…The FTIR spectra of the extracted SF are shown in Figure 3. The indicated spectrum was similar to that of another study using the same extraction technique [54]. The FTIR peaks appeared within the recommended wavelengths.…”

Section: Silk Fibroin Characterizationsupporting

confidence: 79%

“…The random coils showed strong absorption bands at 1625-1660 and 1640-1648 cm −1 (amide I), 1520-1545 cm −1 (amide II), and 1257-1258 cm −1 (amide III), and the β-sheets showed absorption bands at 1625-1640 cm −1 (amide I), 1520-1530 cm −1 (amide II), and 1219-1245 cm −1 (amide III) [45][46][47][48][49][50][51][52][53]. The intense amide band at 3291 cm −1 (beta-sheet) indicated NH bending [54]. The FTIR spectra of the extracted SF are shown in Figure 3.…”

Section: Silk Fibroin Characterizationmentioning

confidence: 99%

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The Possibility of Interlocking Nail Fabrication from FFF 3D Printing PLA/PCL/HA Composites Coated by Local Silk Fibroin for Canine Bone Fracture Treatment

et al. 2020

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The biomaterials polylactic acid (PLA), polycaprolactone (PCL), and hydroxyapatite (HA) were selected to fabricate composite filaments for 3D printing fused filament fabrication (FFF), which was used to fabricate a composite biomaterial for an interlocking nail for canine diaphyseal fractures instead of metal bioinert materials. Bioactive materials were used to increase biological activities and provide a high possibility for bone regeneration to eliminate the limitations of interlocking nails. HA was added to PLA and PCL granules in three ratios according to the percentage of HA: 0%, 5%, and 15% (PLA/PCL, PLA/PCL/5HA, and PLA/PCL/15HA, respectively), before the filaments were extruded. The test specimens were 3D-printed from the extruded composite filaments using an FFF printer. Then, a group of test specimens was coated by silk fibroin (SF) using the lyophilization technique to increase their biological properties. Mechanical, biological, and chemical characterizations were performed to investigate the properties of the composite biomaterials. The glass transition and melting temperatures of the copolymer were not influenced by the presence of HA in the PLA/PCL filaments. Meanwhile, the presence of HA in the PLA/PCL/15HA group resulted in the highest compressive strength (82.72 ± 1.76 MPa) and the lowest tensile strength (52.05 ± 2.44 MPa). HA provided higher bone cell proliferation, and higher values were observed in the SF coating group. Therefore, FFF 3D-printed filaments using composite materials with bioactive materials have a high potential for use in fabricating an interlocking nail for canine diaphyseal fractures.

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Section: Silk Fibroin Characterizationsupporting

confidence: 79%

Section: Silk Fibroin Characterizationmentioning

confidence: 99%

The Possibility of Interlocking Nail Fabrication from FFF 3D Printing PLA/PCL/HA Composites Coated by Local Silk Fibroin for Canine Bone Fracture Treatment

et al. 2020

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“…The FTIR spectrum of the local extracted SF is shown in Figure 9. The FTIR spectrum of the local extracted SF was related to the recommended wavenumbers, as presented in Table 2 [86]. The spectrum was also similar to previous studies with the same extraction technique [15,86].…”

Section: Ftirsupporting

confidence: 72%

“…The FTIR spectrum of the local extracted SF was related to the recommended wavenumbers, as presented in Table 2 [86]. The spectrum was also similar to previous studies with the same extraction technique [15,86]. The FTIR peaks at 1637.7 cm −1 , 1514.3 cm −1 , and 1234.1 cm −1 represent amide I, amide II, and amide III, respectively.…”

Section: Ftirsupporting

confidence: 70%

The 3D-Printed Bilayer’s Bioactive-Biomaterials Scaffold for Full-Thickness Articular Cartilage Defects Treatment

et al. 2020

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The full-thickness articular cartilage defect (FTAC) is an abnormally severe grade of articular cartilage (AC) injury. An osteochondral autograft transfer (OAT) is the recommended treatment, but the increasing morbidity rate from osteochondral plug harvesting is a limitation. Thus, the 3D-printed bilayer’s bioactive-biomaterials scaffold is of major interest. Polylactic acid (PLA) and polycaprolactone (PCL) were blended with hydroxyapatite (HA) for the 3D-printed bone layer of the bilayer’s bioactive-biomaterials scaffold (B-BBBS). Meanwhile, the blended PLA/PCL filament was 3D printed and combined with a chitosan (CS)/silk firoin (SF) using a lyophilization technique to fabricate the AC layer of the bilayer’s bioactive-biomaterials scaffold (AC-BBBS). Material characterization and mechanical and biological tests were performed. The fabrication process consists of combining the 3D-printed structure (AC-BBBS and B-BBBS) and a lyophilized porous AC-BBBS. The morphology and printing abilities were investigated, and biological tests were performed. Finite element analysis (FEA) was performed to predict the maximum load that the bilayer’s bioactive-biomaterials scaffold (BBBS) could carry. The presence of HA and CS/SF in the PLA/PCL structure increased cell proliferation. The FEA predicted the load carrying capacity to be up to 663.2 N. All tests indicated that it is possible for BBBS to be used in tissue engineering for AC and bone regeneration in FTAC treatment.

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“…As can be observed from Figure 7, the band of 1640 cm −1 shifted to 1620 cm −1 with a shoulder at 1640 cm −1 for amide I, after blending SF with curcumin, glutaraldehyde, and glycerol for SF/Gly/GA/Cur film preparation. As the frequency range of 1618–1640 cm −1 was assigned to the enriched β -sheet structure in silk fibroin [37,38,39], and the band at 1620 cm −1 can be attributed to the parallel β -sheet conformation [40], our results verified the changes in the secondary structure of SF and revealed the conformation of SF transit from a poorly defined β -sheet structure to the well-ordered parallel β -sheet conformation after blending with curcumin, glutaraldehyde, and glycerol.…”

Section: Resultsmentioning

confidence: 99%

Fabrication and Characterization of Silk Fibroin/Curcumin Sustained-Release Film

et al. 2019

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In the present work, a sustained-release film composed of silk fibroin (SF), curcumin (Cur), glutaraldehyde (GA), and glycerol (Gly) was prepared successfully for wound dressings. Features relevant to wound dressings of SF/Gly/GA/Cur film were assessed. Physical and chemical properties of the fabricated materials were also characterized. The results showed that the prepared SF/Gly/GA/Cur film demonstrated a good sustained-release performance, flexibility, and gas permeability. In addition, it was found that the prepared SF/Gly/GA/Cur film possessed the capability to effectively inhibit the growth of bacteria and prevent bacterial penetration with a suitable water vapor transmission rate. Furthermore, the prepared composite film was non-cytotoxic, which makes it an ideal material for wound dressings.

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FTIR and WAXD Study of Regenerated Silk Fibroin

Cited by 32 publications

References 19 publications

The Possibility of Interlocking Nail Fabrication from FFF 3D Printing PLA/PCL/HA Composites Coated by Local Silk Fibroin for Canine Bone Fracture Treatment

The Possibility of Interlocking Nail Fabrication from FFF 3D Printing PLA/PCL/HA Composites Coated by Local Silk Fibroin for Canine Bone Fracture Treatment

The 3D-Printed Bilayer’s Bioactive-Biomaterials Scaffold for Full-Thickness Articular Cartilage Defects Treatment

Fabrication and Characterization of Silk Fibroin/Curcumin Sustained-Release Film

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