Fucoidan Exerts Anticancer Effects Against Head and Neck Squamous Cell Carcinoma In Vitro

Abstract

Fucoidans have been reported to exert anticancer effects with simultaneous low toxicity against healthy tissue. That correlation was observed in several cancer models, however, it has never been investigated in head and neck cancer before. To magnify the efficacy of conventional therapy, the administration of agents like fucoidan could be beneficial. The aim of this study was to evaluate the anticancer effect of Fucus vesiculosus (FV) extract alone and with co-administration of cisplatin in head and neck squamous cell carcinoma (HNSCC) in vitro. MTT assay results revealed an FV-induced inhibition of proliferation in all tested cell lines (H103, FaDu, KB). Flow cytometric cell cycle analysis showed an FV-induced, dose-dependent arrest in either S/G2 phase (H103, FaDu) or G1 arrest (KB). Furthermore, a dose-dependent gain in apoptotic fraction was observed. Western blot analysis confirmed the induction of apoptosis. A significant dose-dependent increase in reactive oxygen species (ROS) production was revealed in the H103 cell line, while FaDu cells remained unresponsive. On the contrary, an HPV-positive cell line, KB, demonstrated a dose-dependent decrease in ROS synthesis. Moreover, fucoidan enhanced the response to cisplatin (synergistic effect) in all cell lines with the HPV-positive one (KB) being the most sensitive. These results have been confirmed by flow-cytometric apoptosis analysis. In conclusion, we confirmed that fucoidan exhibits anticancer properties against HNSCC, which are manifested by the induction of apoptosis, regulation of ROS production, cell cycle arrest, and inhibition of proliferation.

Fucoidan affects oral squamous cell carcinoma cell functions in vitro by regulating FLNA‐derived circular RNA

“…47 Our results demonstrated that fucoidan could induce G 2 phase arrest, similar to that obtained by Blaszczak et al using FaDu cells. 48 Other evidence is available indicating that fucoidan mediates cell death by inducing apoptosis. Treatment with a LMWF resulted in apoptosis in breast cancer cells due to decrease of antiapoptotic proteins.…”

Antitumor Effects of Fucoidan Via Apoptotic and Autophagic Induction on HSC-3 Oral Squamous CellCarcinoma

“…Many previous studies suggested that fucoidan, a sulfated polysaccharide extracted from brown algae, exerts the suppression of growth and proliferation of human cancer cells through apoptotic mechanisms (Atashrazm et al, 2016;Blaszczak et al, 2018;Han et al, 2015;Kim et al, 2010;Narayani et al, 2019;Park et al, 2011). Moreover, one study reported the effect of fucoidan in reducing the invasion of oral squamous cell carcinoma CAL27 cell line (Lin et al, 2017).…”

“…Oral squamous cell carcinoma (OSCC) is one of the cancers that can cause mortality worldwide (Torre et al, 2015). Smoking, tobacco use, alcohol consumption, and HPV infection are the risk factors of oral cavity cancer (Blot et al, 1988;Hashibe et al, 2009). Although treatment methods have developed over the last few decades, the prognosis for patients with OSCC is still moderate or poor.…”

Overcoming Resistance to Platinum-Based Drugs in Ovarian Cancer by Salinomycin and Its Derivatives—An In Vitro Study

“…For the evaluation of cell proliferation inhibition, we used the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay as previously described [59]. Briefly, A2780 WT and CDDP (2000 cells/well), SK-OV-3 WT and CDDP (800 cells/well), and MRC-5 pd19 (3000 cells/well) were seeded onto a 96-well plate for overnight until the cells attached.…”