Fucoidan/VEGF-based surface modification of decellularized pulmonary heart valve improves the antithrombotic and re-endothelialization potential of bioprostheses

Marinval, Nicolas; Morenc, Małgorzata; Labour, Marie-Noëlle; Samotus, A.; Mzyk, Aldona; Ollivier, Véronique; Maire, Murielle; Jesse, Katarzyna; Bassand, Kevin; Niemiec-Cyganek, Aleksandra; Haddad, Oualid; Jacob, Marie Paule; Chaubet, Frédéric; Charnaux, Nathalie; Wilczek, Piotr; Hlawaty, Hanna

doi:10.1016/j.biomaterials.2018.01.054

Cited by 73 publications

(53 citation statements)

References 60 publications

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Section: Discussionmentioning

confidence: 99%

“…Several VEGF‐based surface modification strategies have been reported to improve endothelialization of BHVs . A fucoidan/VEGF multilayer film‐coated valve scaffold has been developed to make a functional heart valve bioprosthesis with improved antithrombotic and reendothelialization potential . A heparin‐VEGF polyelectrolyte multilayer film has been used to cover a decellularized porcine aortic valve to decrease platelet adhesion, and the modified valve exhibited excellent biocompatibility for endothelial progenitor cells .…”

Section: Discussionmentioning

confidence: 99%

“…When pericardia are used for BHVs in the clinic, they are likely to cause serious pathological calcification and result in clinical failure . One major reason for this is the lack of endothelialization . Endothelial cells have an important role in the regulation of hemostatic balance, coagulation, and the local behavior of platelets and leucocytes.…”

Section: Introductionmentioning

confidence: 99%

“…Vascular endothelial growth factor (VEGF) is known to promote endothelial proliferation and adhesion . Fucoidan/VEGF‐based surface modification of decellularized pulmonary heart valves shows improved reendothelialization potential of bioprostheses . Polyelectrolyte multilayer films based on VEGF show enhanced valve scaffold biocompatibility by increasing the adhesive and proliferative properties of human umbilical vein endothelial cells (HUVECs) .…”

Section: Introductionmentioning

confidence: 99%

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Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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Bioprosthetic heart valves (BHVs) used in the clinic are mostly fixed by glutaraldehyde and the lack of endothelialization is a major problem for glutaraldehyde‐fixed pericardia. Hyaluronic acid is a major glycosaminoglycan that exists in native heart valves. Coupled with its inherent biocompatibility, it may enhance endothelial adhesion and proliferation when associated with vascular endothelial growth factor (VEGF). In this study, an optimized system is developed to improve the endothelialization of glutaraldehyde‐fixed pericardium. A hybrid pericardium with VEGF‐loaded hyaluronic acid hydrogel coating is developed by the crosslinking of 1,4‐butanediol diglycidyl ether. The adhesion and growth potential of human umbilical vein endothelial cells (HUVECs) on pericardia, platelet adhesion, and calcification by an in vivo rat subdermal implantation model are investigated. The results show improved HUVEC adhesion and proliferation, less platelet adhesion, and less calcification for hybrid pericardium by introducing the coating of VEGF‐loaded hyaluronic acid hydrogel. Thus, the coating of VEGF‐loaded hyaluronic acid hydrogel on pericardium is a promising approach to obtain bioprosthetic valves for clinical applications with increased endothelialization and antithrombotic and anticalcification properties.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Lei

Deng

Tang

et al. 2019

Macromolecular Bioscience

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“…The biological activity of the released VEGF was tested on HUVECs (Marinval et al, 2018). Treatment of HUVEC cells with VEGF demonstrated that released VEGF stimulates cell proliferation as effectively as free VEGF (Figure 2C).…”

Section: Biological Activity Of Gf/cmentioning

confidence: 99%

Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration

Jimi

Jaguparov

Nurkesh

et al. 2020

Front. Bioeng. Biotechnol.

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Growth factors and cytokines that are secreted by cells play a crucial role in the complex physiological reaction to tissue injury. The ability to spatially and temporally control their actions to maximize regenerative benefits and minimize side effects will help accelerate wound healing and improve tissue regeneration. In this study, the sequential targeted delivery of growth factor/cytokine combinations with regulatory functions on inflammation and tissue regeneration was examined using an internal splint wound healing model. Four examined growth factors and cytokines were effectively incorporated into a novel chitosan-based cryogel, which offered a controlled and sustained release of all factors while maintaining their biological activities. The cryogels incorporated with inflammation modulatory factors (IL-10 and TGF-β) and with wound healing factors (VEGF and FGF) were placed on the wound surface on day 0 and day 3, respectively, after wound initiation. Although wound area gradually decreased in all groups over time, the area in the cryogel group with growth factor/cytokine combinations was significantly reduced starting on day 7 and reached about 10% on day 10, as compared to 60-65% in the control groups. Sequential delivery of inflammation modulatory and wound healing factors enhanced granulation tissue formation, as well as functional neovascularization, leading to regenerative epithelialization. Collectively, the chitosan-based cryogel can serve as a controlled release system for sequential delivery of several growth factors and cytokines to accelerate tissue repair and regeneration.

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Silk Sericin is a Potent, Multifunctional Biomaterial for Endothelialization of Tissue‐Engineered Heart Valves

Song,

Wang,

et al. 2024

Adv Funct Materials

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Endothelialization is important for offering an anticoagulant surface, which is crucial for the construction of tissue‐engineered heart valves. Silk sericin is extensively investigated in the biomedical field due to its remarkable biological activities and diverse functional groups, favoring chemical modifications for the formation of versatile constructs. Herein, a sericin covalently modified valve (SCMV) is successfully fabricated by coupling maleylated silk sericin (MSS) with thiolated decellularized heart valve (DHV) through a Michael addition reaction. The results demonstrate that SCMV exhibits a smooth surface, higher hydrophilicity, and excellent resistance to platelets adhesion compared to DHV. Additionally, SCMV promotes endothelial cells (ECs) adhesion under both static and flow conditions and enhances their survival in a mouse subcutaneous implant model. The study also discovered that MSS‐stimulated ECs exhibit increased RhoA activation, expression of rho‐associated protein kinase 2 (ROCK2) and phosphorylated myosin light chain 2 (MLC2), actin polymerization, and cell adhesion; whereas actin organization and cell adhesion are significantly reduced by treatment with Y‐27632, a ROCK inhibitor. Furthermore, several integrin subunits, including α1, α2, α3, α5, α6, β1, β3, and β5, are involved in this regulatory process. Collectively, the findings highlight the potential application of silk sericin in promoting endothelialization of DHV while uncovering novel mechanisms underlying its regulatory effect on ECs adhesion.

show abstract

Fucoidan/VEGF-based surface modification of decellularized pulmonary heart valve improves the antithrombotic and re-endothelialization potential of bioprostheses

Cited by 73 publications

References 60 publications

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Hybrid Pericardium with VEGF‐Loaded Hyaluronic Acid Hydrogel Coating to Improve the Biological Properties of Bioprosthetic Heart Valves

Sequential Delivery of Cryogel Released Growth Factors and Cytokines Accelerates Wound Healing and Improves Tissue Regeneration

Silk Sericin is a Potent, Multifunctional Biomaterial for Endothelialization of Tissue‐Engineered Heart Valves

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