Fucoidans: The importance of processing on their anti-tumoral properties

Torres, M.D.; Flórez‐Fernández, Noelia; Simón‐Vázquez, Rosana; Giménez-Abián, Juan F.; Díaz, José-Luis; González-Fernández, África; Domı́nguez, Herminia

doi:10.1016/j.algal.2019.101748

Cited by 27 publications

(15 citation statements)

References 209 publications

(315 reference statements)

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“…Based on its effects on different types of tumor cells, fucoxanthin is currently being studied as a new anticancer compound [ 20 ]. One of the aspects of most conventional therapies that requires improvement is the selectivity of anti-tumor agents.…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Advances in Studies on the Pharmacological Activities of Fucoxanthin

et al. 2020

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Fucoxanthin is a natural carotenoid derived mostly from many species of marine brown algae. It is characterized by small molecular weight, is chemically active, can be easily oxidized, and has diverse biological activities, thus protecting cell components from ROS. Fucoxanthin inhibits the proliferation of a variety of cancer cells, promotes weight loss, acts as an antioxidant and anti-inflammatory agent, interacts with the intestinal flora to protect intestinal health, prevents organ fibrosis, and exerts a multitude of other beneficial effects. Thus, fucoxanthin has a wide range of applications and broad prospects. This review focuses primarily on the latest progress in research on its pharmacological activity and underlying mechanisms.

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Section: Pharmacological Activitiesmentioning

confidence: 99%

Advances in Studies on the Pharmacological Activities of Fucoxanthin

et al. 2020

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“…Both fucoidan polysaccharides and fucoidan oligosaccharides have been reported to display a broad array of valuable biological activities [ 5 , 6 ]. These effects have mainly been shown in vitro in different cell systems, but in some cases also in vivo in animal systems, including anticancer [ 7 , 8 , 9 , 10 , 11 , 12 ], anti-inflammatory [ 1 , 13 ], anticoagulant [ 14 , 15 ], immune-modulatory [ 16 ], and most recently, potentially protective effects against age-related macular degeneration induced vision loss [ 17 ]. Although details of the structure-function of fucoidans remain unclear, it is evident that structural integrity is critical for achieving particular effects [ 1 , 2 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of a New GH107 Endo-α-(1,4)-Fucoidanase from the Marine Bacterium Formosa haliotis

et al. 2020

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Fucoidans from brown macroalgae are sulfated fucose-rich polysaccharides, that have several beneficial biological activities, including anti-inflammatory and anti-tumor effects. Controlled enzymatic depolymerization of the fucoidan backbone can help produce homogeneous, defined fucoidan products for structure-function research and pharmaceutical uses. However, only a few endo-fucoidanases have been described. This article reports the genome-based discovery, recombinant expression in Escherichia coli, stabilization, and functional characterization of a new bacterial endo-α-(1,4)-fucoidanase, Fhf1, from Formosa haliotis. Fhf1 catalyzes the cleavage of α-(1,4)-glycosidic linkages in fucoidans built of alternating α-(1,3)-/α-(1,4)-linked l-fucopyranosyl sulfated at C2. The native Fhf1 is 1120 amino acids long and belongs to glycoside hydrolase (GH) family 107. Deletion of the signal peptide and a 470 amino acid long C-terminal stretch led to the recombinant expression of a robust, minimized enzyme, Fhf1Δ470 (71 kDa). Fhf1Δ470 has optimal activity at pH 8, 37–40 °C, can tolerate up to 500 mM NaCl, and requires the presence of divalent cations, either Ca2+, Mn2+, Zn2+ or Ni2+, for maximal activity. This new enzyme has the potential to serve the need for controlled enzymatic fucoidan depolymerization to produce bioactive sulfated fucoidan oligomers.

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“…Furthermore, the improvement of fucoidans activity was investigated, targeting several points. Among these was the modification of the chemical structure of the native fucoidans scaffolding, including depolymerization [57,58] and over-sulfation [59]. These modifications could be attempted chemically [60], enzymatically [35,61] or physically [62].…”

Section: Of 22mentioning

confidence: 99%

“…Others patented production techniques that have assisted in the marketing of several commercial fucoidans by well-known companies (e.g., Sigma-Aldrich ® , Algues and Mer and Marinova ® ) derived from Fucus vesiculosus and other brown algae species [54][55][56].Furthermore, the improvement of fucoidans activity was investigated, targeting several points. Among these was the modification of the chemical structure of the native fucoidans scaffolding, including depolymerization [57,58] and over-sulfation [59]. These modifications could be attempted chemically [60], enzymatically [35,61] or physically [62].…”

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confidence: 99%

Fucoidans: Downstream Processes and Recent Applications

Zayed

Ulber

2020

Marine Drugs

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Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, physicochemical properties, biological properties and industrial applications. Since a universal protocol for fucoidans production has not been established yet, all the currently used processes were presented and justified. The current article complements our previous articles in the fucoidans field, provides an updated overview regarding the different downstream processes, including pre-treatment, extraction, purification and enzymatic modification processes, and shows the recent non-traditional applications of fucoidans in relation to their characters. of 22reproductive, immune and cell-to-cell communicative roles [23]. As recommended by the International Union of Pure and Applied Chemistry (IUPAC), fucoidans is a general term used to describe sulfated L-fucose-based polymers including sulfated fucans cited by the Swedish scholar Kylin, as well as other fucose-rich sulfated heteropolysaccharides [23,28]. Their chemical structures, in terms of monomeric composition and branching, are quite simple in marine invertebrates compared to their analogues in brown algae [13,29].Hundreds of articles have thoroughly discussed and reviewed the biological, pharmacological and pharmaceutical applications of fucoidans [30][31][32][33], including nanomedicine, [34] which has made it a hot topic in the last few decades [35][36][37]. All these studies tried to investigate fucoidans molecular mechanisms in relation to their chemical structure and physicochemical properties. Therefore, different hypotheses were suggested for each activity, such as anti-tumor [31,[38][39][40], anti-coagulant [41,42], anti-viral [43,44] and anti-inflammatory activity [45,46]. These investigations revealed that various factors are relevant, such as molecular weight, sulfation pattern, sulfate content and monomeric composition [47][48][49]. For example, different fractions were produced with different physicochemical properties in our previous experiments; sulfation pattern and sulfate content were highly related to anti-viral and cytotoxic activities against HSV-1 and Caco-2 cell lines, respectively, while molecular weight and sugar composition were potential factors in anti-coagulation activity [41,50]. In addition, degree of purity was reported as an influential factor [32], where co-extracted contaminants (e.g., phlorotannins or polyphenols) could lead to significant interference in anti-oxidant activity and, consequently, cosmetic applications [51,52].Therefore, several key production challenges regarding fucoidans were discussed in our last review article in order to obtain a product that follows the universal good manufactured practice (GMP) guidelines. The article discussed sources of heterogeneity in extracted fucoidans, including the different biotic (e.g., biogenic, geographical and seas...

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Fucoidans: The importance of processing on their anti-tumoral properties

Cited by 27 publications

References 209 publications

Advances in Studies on the Pharmacological Activities of Fucoxanthin

Advances in Studies on the Pharmacological Activities of Fucoxanthin

Functional Characterization of a New GH107 Endo-α-(1,4)-Fucoidanase from the Marine Bacterium Formosa haliotis

Fucoidans: Downstream Processes and Recent Applications

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