Full-field laser–Doppler imaging and its physiological significance for tissue blood perfusion

Binzoni, Tiziano; Ville, Dimitri Van De

doi:10.1088/0031-9155/53/23/003

Cited by 13 publications

(13 citation statements)

References 42 publications

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“…Some of the issues are that these devices' probes require direct contact with the skin, measurements are not highly reproducible, and the sampled region is very small (1-2 mm). Laser Doppler imaging (LDI) technique is based on extending the principle of laser Doppler flowcytometry (LDF) which allows measurement from a distance of a larger skin area (Binzoni and Van De Ville, 2008;Svedman et al, 1998;Wårdell et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers

Jafari

Schawkat

Ville

et al. 2014

Microvascular Research

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We used real-time LDI to study regional variations in microcirculatory perfusion in healthy candidates to establish a new methodology for global perfusion body mapping that is based on intra-individual perfusion index ratios. Our study included 74 (37 female) healthy volunteers aged between 22 and 30 years (mean 24.49). Imaging was performed using a recent microcirculation-imaging camera (EasyLDI) for different body regions of each volunteer. The perfusion values were reported in Arbitrary Perfusion Units (APU). The relative perfusion indexes for each candidate's body region were then obtained by normalization with the perfusion value of the forehead. Basic parameters such as weight, height, and blood pressure were also measured and analyzed. The highest mean perfusion value was reported in the forehead area (259.21 APU). Mean perfusion in the measured parts of the body correlated positively with mean forehead value, while there was no significant correlation between forehead blood perfusion values and room temperature, BMI, systolic blood pressure and diastolic blood pressure (p = 0.420, 0.623, 0.488, 0.099, respectively). Analysis of the data showed that perfusion indexes were not significantly different between male and female volunteers except for the ventral upper arm area (p = .001). LDI is a non-invasive, fast technique that opens several avenues for clinical applications. The mean perfusion indexes are useful in clinical practice for monitoring patients before and after surgical interventions. Perfusion values can be predicted for different body parts for patients only by taking the forehead perfusion value and using the perfusion index ratios to obtain expected normative perfusion values.

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Section: Discussionmentioning

confidence: 99%

Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers

Jafari

Schawkat

Ville

et al. 2014

Microvascular Research

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“…A full medical history was taken and the scar photographed. Baseline noninvasive imaging of the scar was performed using spectrophotometric intracutaneous analysis (SIAscopy) (Astron Clinica, Cambridge, U.K.) and full‐field laser perfusion imaging (FLPI; Moor Instruments, Axminster, U.K.) (Fig. ).…”

Section: Methodsmentioning

confidence: 99%

An abnormality in glucocorticoid receptor expression differentiates steroid responders from nonresponders in keloid disease

et al. 2015

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SummaryBackgroundGlucocorticoids (GCs) are first‐line treatment for keloid disease (KD) but are limited by high incidence of resistance, recurrence and undesirable side‐effects. Identifying patient responsiveness early could guide therapy.MethodsNineteen patients with KD were recruited at week 0 (before treatment) and received intralesional steroids. At weeks 0, 2 and 4, noninvasive imaging and biopsies were performed. Responsiveness was determined by clinical response and a significant reduction in vascular perfusion following steroid treatment, using full‐field laser perfusion imaging (FLPI). Responsiveness was also evaluated using (i) spectrophotometric intracutaneous analysis to quantify changes in collagen and melanin and (ii) histology to identify changes in epidermal thickness and glycosaminoglycan (GAG) expression. Biopsies were used to quantify changes in glucocorticoid receptor (GR) expression using quantitative reverse transcriptase polymerase chain reaction, immunoblotting and immunohistochemistry.ResultsAt week 2, the FLPI was used to separate patients into steroid responsive (n = 12) and nonresponsive groups (n = 7). All patients demonstrated a significant decrease in GAG at week 2 (P < 0·05). At week 4, responsive patients exhibited significant reduction in melanin, GAG, epidermal thickness (all P < 0·05) and a continued reduction in perfusion (P < 0·001) compared with nonresponders. Steroid‐responsive patients had increased GR expression at baseline and showed autoregulation of GR compared with nonresponders, who showed no change in GR transcription or protein.ConclusionsThis is the first demonstration that keloid response to steroids can be measured objectively using noninvasive imaging. FLPI is a potentially reliable tool to stratify KD responsiveness. Altered GR expression may be the mechanism gating therapeutic response.

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“…where ω 0 and ω 2 have been expressed using equations (10) and (8), respectively. Equation 11 and V trans (normal velocity distribution plus a bulk translational movement) can be written as (equation 14in Binzoni and Van De Ville 2008)…”

Section: Link Between ω N and The Physiological Parametersmentioning

confidence: 99%

A fast time-domain algorithm for the assessment of tissue blood flow in laser-Doppler flowmetry

Binzoni¹,

Seelamantula²,

Ville³

2010

Phys. Med. Biol.

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In this study, we derive a fast, novel time-domain algorithm to compute the nth-order moment of the power spectral density of the photoelectric current as measured in laser-Doppler flowmetry (LDF). It is well established that in the LDF literature these moments are closely related to fundamental physiological parameters, i.e. concentration of moving erythrocytes and blood flow. In particular, we take advantage of the link between moments in the Fourier domain and fractional derivatives in the temporal domain. Using Parseval's theorem, we establish an exact analytical equivalence between the time-domain expression and the conventional frequency-domain counterpart. Moreover, we demonstrate the appropriateness of estimating the zeroth-, first- and second-order moments using Monte Carlo simulations. Finally, we briefly discuss the feasibility of implementing the proposed algorithm in hardware.

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Full-field laser–Doppler imaging and its physiological significance for tissue blood perfusion

Cited by 13 publications

References 42 publications

Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers

Relative indexes of cutaneous blood perfusion measured by real-time laser Doppler imaging (LDI) in healthy volunteers

An abnormality in glucocorticoid receptor expression differentiates steroid responders from nonresponders in keloid disease

A fast time-domain algorithm for the assessment of tissue blood flow in laser-Doppler flowmetry

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