Optics in the Life Sciences 2015
DOI: 10.1364/boda.2015.jt3a.8
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Full-field Transmission-type Angle-deviation Optical Microscope with Reflectivity-Height Transformation

Abstract: This full-field transmission-type three-dimensional (3D) optical microscope is constructed based on the angle deviation method (ADM) and the algorithm of reflectivity-height transformation (RHT). The surface height is proportional to the deviation angle of light passing through the object. The angle deviation and surface height can be measured based on the reflectivity closed to the critical angle using a parallelogram prism and two CCDs.

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