Full genomic sequence analysis of swine genotype 3 hepatitis E virus isolated from Shanghai

Construction of an Infectious cDNA Clone of a Swine Genotype 3 HEV Strain Isolated in Shanghai, China

“…RACE was used to amplify the 5′ and 3′ UTR ends, and the remaining seven fragments were acquired by overlapping PCR as described previously [40]. A subclone of the human hepatoma cell line Huh7 was grown as a monolayer in Dulbecco's modified Eagle's medium (DMEM) supplemented with 2 m M L -glutamine and 8% fetal bovine serum in a 5% CO 2 incubator at 37°.…”

“…Nine previously amplified fragments [40] encompassing the entire SAAS-JDY5 genome were used as the template for the construction of a full-length cDNA clone of HEV adopting the strategy outlined in figure 1. Firstly, fragment 5′-2 (F5′-2) was obtained by overlapping PCR using primers 5′-S and I2-A (table 1) and three overlapping fragments (5′, 1, 2) as template.…”

“…In 2007, swine genotype 3 HEV was reported in Shanghai [25], and a strain designated SAAS-JDY5 was isolated later for full-length genome sequence analysis [40]. More recently, a human genotype 3 HEV infection was reported in Jiangsu Province adjoining the municipality of Shanghai [41] and, since this genotype is evidently an emerging infectious disease agent in China for both animals and humans, there is a clear need for more research on the mechanisms of cross-species transmission and the evolution of genotype 3 HEV in the region.…”

Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China

“…Notably, the highest genomic nucleotide similarity of 87.9% was revealed between ZhJ-PJ050-3 and the reported genotype 3 HEV strain SAAS-JDY5 (GenBank, accession No. FJ527832) isolated from Shanghai, China (Si et al, 2009). The ORF1, ORF2, and ORF3 sequences of the two Chinese strains were 87.9%, 86.1%, and 93.6% similar, respectively.…”

“…However, infections with HEV 3 have been found more commonly in recent years in China (Liu et al, 2012;Zhang et al, 2013). To date, only one full genome of Chinese swine genotype 3 HEV strain from Shanghai has been documented (Si et al, 2009). We report here the first full genome sequence of a genotype 3 swine HEV strain from Zhejiang, China.…”