Full-Length Genome Sequence of Type M/
            <i>emm83</i>
            Group A Streptococcus pyogenes Strain STAB1101, Isolated from Clustered Cases in Brittany

Soriano, Nicolas; Vincent, P; Auger, Gabriel; Cariou, Marie-Estelle; Moullec, Séverine; Lagente, Vincent; Ygout, J.-F.; Kayal, Samer; Faili, Ahmad

doi:10.1128/genomea.01459-14

Cited by 7 publications

(9 citation statements)

References 11 publications

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“…STAB1101, whose genome was published while this article was in preparation (23). Some of the newly sequenced strains had large rearrangements which occurred at repetitive elements, such as prophages and rRNA operons (see Fig.…”

Section: Fig 2 (A) Emm Type Distribution Of Isolates Causing Igas Dismentioning

confidence: 99%

High Incidence of Invasive Group A Streptococcus Disease Caused by Strains of Uncommon emm Types in Thunder Bay, Ontario, Canada

et al. 2016

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An outbreak of type emm59 invasive group A Streptococcus (iGAS) disease was declared in 2008 in Thunder Bay District, Northwestern Ontario, 2 years after a countrywide emm59 epidemic was recognized in Canada. Despite a declining number of emm59 infections since 2010, numerous cases of iGAS disease continue to be reported in the area. We collected clinical information on all iGAS cases recorded in Thunder Bay District from 2008 to 2013. We also emm typed and sequenced the genomes of all available strains isolated from 2011 to 2013 from iGAS infections and from severe cases of soft tissue infections. We used whole-genome sequencing data to investigate the population structure of GAS strains of the most frequently isolated emm types. We report an increased incidence of iGAS in Thunder Bay compared to the metropolitan area of Toronto/Peel and the province of Ontario. Illicit drug use, alcohol abuse, homelessness, and hepatitis C infection were underlying diseases or conditions that might have predisposed patients to iGAS disease. Most cases were caused by clonal strains of skin or generalist emm types (i.e., emm82, emm87, emm101, emm4, emm83, and emm114) uncommonly seen in other areas of the province. We observed rapid waxing and waning of emm types causing disease and their replacement by other emm types associated with the same tissue tropisms. Thus, iGAS disease in Thunder Bay District predominantly affects a select population of disadvantaged persons and is caused by clonally related strains of a few skin and generalist emm types less commonly associated with iGAS in other areas of Ontario. Group A Streptococcus (GAS) causes a wide variety of diseases ranging in severity from uncomplicated pharyngitis to lifethreatening necrotizing fasciitis and streptococcal toxic shock syndrome (1). More than 240 GAS emm types are recognized based on the sequence of the hypervariable 5= end of gene emm, encoding M protein, a major GAS virulence factor with antiphagocytic properties (2-4). Beginning in 2006, Canada experienced an epidemic of invasive GAS (iGAS) infections caused by strains of the previously rare emm59 type (5). A hypervirulent clone, which later disseminated to several areas of the United States, was shown to be responsible for hundreds of cases countrywide (6-10). Invasive type emm59 strains were frequently isolated from soft tissue infections and were recovered in higher percentages from patients with distinctive risk factors, including alcohol abuse, homelessness, hepatitis C virus (HCV) infection, and intravenous drug use (IVDU) (5, 6). In the province of Ontario, most emm59 iGAS infections occurred in Thunder Bay, the most populous municipality in the Northwestern area of the province (population, approximately 110,000), and the regional center of the Thunder Bay District (TBD), which extends over an area of 103,720 square kilometers.The number of emm59 iGAS cases in Canada, particularly in Ontario, has been in continuous decline since 2010 (8). However, TBD public health authorities continue to observe ra...

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Section: Fig 2 (A) Emm Type Distribution Of Isolates Causing Igas Dismentioning

confidence: 99%

High Incidence of Invasive Group A Streptococcus Disease Caused by Strains of Uncommon emm Types in Thunder Bay, Ontario, Canada

et al. 2016

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“…The marS gene is conserved throughout GAS, and the binding site in the 5′ UTR of mga is conserved in several GAS emm types as determined by BLAST 23 analysis. Amongst others, these include emm18 , emm28 , emm 59, and emm83 , which are responsible for diseases as diverse as acute rheumatic fever, puerperal sepsis, severe invasive disease, and skin tissue infections 47 – 50 . Binding of sRNAs to their target mRNAs is a typical characteristic of trans-acting sRNAs in many bacteria.…”

Section: Discussionmentioning

confidence: 99%

The Regulatory Small RNA MarS Supports Virulence of Streptococcus pyogenes

Pappesch

Warnke

Mikkat³

et al. 2017

Sci Rep

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Small regulatory RNAs (sRNAs) play a role in the control of bacterial virulence gene expression. In this study, we investigated an sRNA that was identified in Streptococcus pyogenes (group A Streptococcus, GAS) but is conserved throughout various streptococci. In a deletion strain, expression of mga, the gene encoding the multiple virulence gene regulator, was reduced. Accordingly, transcript and proteome analyses revealed decreased expression of several Mga-activated genes. Therefore, and because the sRNA was shown to interact with the 5′ UTR of the mga transcript in a gel-shift assay, we designated it MarS for m ga-activating regulatory sRNA. Down-regulation of important virulence factors, including the antiphagocytic M-protein, led to increased susceptibility of the deletion strain to phagocytosis and reduced adherence to human keratinocytes. In a mouse infection model, the marS deletion mutant showed reduced dissemination to the liver, kidney, and spleen. Additionally, deletion of marS led to increased tolerance towards oxidative stress. Our in vitro and in vivo results indicate a modulating effect of MarS on virulence gene expression and on the pathogenic potential of GAS.

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“…For each strain (STAB090229, STAB120304, and STAB14018), a total of 15,201,760, 11,304,212, and 14,127,274 high-quality reads, giving average coverages of 1,253-, 1,068-, and 927-fold, respectively, were assembled using CLC Genomics Workbench v.6 software. The resulting assembly consisted of 25, 25, and 30 contigs oriented on the basis of available sequences of GAS, and 21, 23, and 25 persisting gaps, respectively, were filled as previously described ( 6 , 7 ). Genome annotations were performed in parallel by using the Rapid Annotations using Subsystems Technology (RAST) server ( 8 ) and NCBI-PGAAP ( http://ncbi.nlm.nih.gov/genome/annotation_prok ).…”

Section: Genome Announcementmentioning

confidence: 99%

Full Sequencing and Genomic Analysis of Three emm 75 Group A Streptococcus Strains Recovered in the Course of an Epidemiological Shift in French Brittany

et al. 2017

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Full-Length Genome Sequence of Type M/ emm83 Group A Streptococcus pyogenes Strain STAB1101, Isolated from Clustered Cases in Brittany

Cited by 7 publications

References 11 publications

High Incidence of Invasive Group A Streptococcus Disease Caused by Strains of Uncommon emm Types in Thunder Bay, Ontario, Canada

High Incidence of Invasive Group A Streptococcus Disease Caused by Strains of Uncommon emm Types in Thunder Bay, Ontario, Canada

The Regulatory Small RNA MarS Supports Virulence of Streptococcus pyogenes

Full Sequencing and Genomic Analysis of Three emm 75 Group A Streptococcus Strains Recovered in the Course of an Epidemiological Shift in French Brittany

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