Full-length genomic characterization and molecular evolution of canine parvovirus in China

Zhou, Ling; Tang, Qun; Shi, Lisong; Kong, Miaomiao; Liang, Lin; Mao, Qianqian; Bu, Bin; Yao, Lunguang; Zhao, Kai; Cui, Shangjin; Leal, Élcio

doi:10.1007/s11262-016-1309-y

Cited by 16 publications

(9 citation statements)

References 29 publications

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“…CPV-2 is a common etiological agent that causes severe gastroenteritis in puppies and coinfects with more than one enteric pathogen (Zhou et al, 2016). T. Thaiwong hypothesizes that CPV-2 infection predisposed dogs to CaCV-1 infection and ultimately resulted in more severe diseases (Thaiwong et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

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A B S T R A C TPorcine circovirus type 3 (PCV3) is an emerging circovirus species associated with several diseases. The study aimed to investigate the frequency of porcine circovirus 3 (PCV3) and its coinfection with canine parvovirus type 2 (CPV-2) in dogs in the Guangxi province from 2015 to 2017, China, and to examine the genome diversity of PCV3. Using polymerase chain reaction (PCR) amplification and sequencing, 96 of 406 (23.6%)samples were positive for PCV3, 38 out of 406 (9.4%) samples were coinfected with both PCV3 and CPV-2. The CPV-positive rate was significantly higher in the PCV3-positive samples than in the non-PCV3 samples, and the difference was extremely significant (P < 0.01). The complete genome (n=4) and ten capsid genes (n=10) of PCV3 were sequenced. Multiple sequence alignment results showed that these sequences shared 98.5-100% nucleotide similarity with the reference genome sequence and 97.5-100% nucleotide similarity with the reference capsid gene sequence. PCV3 was classified into two different genotypes, according to phylogenetic analysis based on the whole genome. These strains were clustered in PCV3a, showing a close relationship with PCV3-US/SD2016. Surprisingly, we separately analyzed these PCV3 strains from the Guangxi province and found that the dog and pig PCV3 are from different branches. In summary, this was the first seroprevalence and genetic investigation of PCV3 in dogs in the Guangxi province, China, and the first complete genome PCV3 from dogs obtained in the world. The results provide insights into the epidemiology and pathogenesis of this important virus.

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Section: Discussionmentioning

confidence: 99%

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

et al. 2019

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“…Subsequently, widespread outbreaks of canine haemorrhagic enteritis occurred throughout the country and CPV‐2 infection emerged as an important infectious disease of dogs because of the high morbidity and mortality associated with the virus (Wu et al., ; Xu et al., ; Zhao et al., , , ). In 2015, new CPV‐2a and new CPV‐2b were discovered with full‐length genome analysis in Gansu province (Zhou et al., ). To investigate further the genomic characteristics of CPV‐2 in China, CPV‐2 identified from pet dogs with clinical signs of vomiting and haemorrhagic diarrhoea in the Animal Hospital of Chengdu in Sichuan province were characterized in this study.…”

Section: Introductionmentioning

confidence: 99%

Genome sequence characterization of canine parvoviruses prevalent in the Sichuan province of China

Zhuang

Qiu

Pan

et al. 2019

Transbound Emerg Dis

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Canine parvovirus 2 (CPV‐2) infection is responsible for large numbers of animal deaths worldwide and is one of the most dangerous infectious diseases in young puppies. Twenty‐four rectal swabs were collected from dogs with clinical signs of vomiting and haemorrhagic diarrhoea and were initially verified to be infected with CPV‐2 using colloidal gold test strips. From the 24 CPV‐positive samples, complete genome of 5050–5054 nucleotides was sequenced with a next‐generation sequencing platform. Characteristics of the Open Reading Frames from different CPV‐2 strains detected in this study were analyzed. Several VP2 point mutations were discovered, and demonstrated the co‐circulation of new CPV‐2a, new CPV‐2b and CPV‐2c in Sichuan province of China. The analysis results of the Chinese CPV‐2 retrieved from the NCBI nucleotide, showed that new CPV‐2a has become the predominant variant in some provinces of China. Phylogenetic analysis of global VP2 and NS1 nucleotide sequences revealed certain correlations among geographical regions, types and circulating time, which lays the foundation for further research concerning the epidemiology, genetic variation, vaccination and molecular evolutionary relationships of the CPV‐2 identified at different times and from different regions.

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“…The viral particle shows a spherical capsid and non-enveloped structure, with a diameter of 25 nm. The genome consists of a linear and single-stranded DNA molecule that is 5.2 kb in length, with two open reading frames (ORF) [ 2 , 6 ]. The first ORF encodes two non-structural proteins (NS1 and NS2) and the second ORF encodes two structural proteins (VP1 and VP2) that assemble the capsid with 54 units of VP1 and 6 units of VP2 [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador

Torre¹,

Mafla²,

Puga³

et al. 2018

Vet World

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AimThe objective of this study was to determine the presence of the variants of canine parvovirus (CPV)-2 in the city of Quito, Ecuador, due to the high domestic and street-type canine population, and to identify possible mutations at a genetic level that could be causing structural changes in the virus with a consequent influence on the immune response of the hosts.Materials and MethodsThirty-five stool samples from different puppies with characteristic signs of the disease and positives for CPV through immunochromatography kits were collected from different veterinarian clinics of the city. Polymerase chain reaction and DNA sequencing were used to determine the mutations in residue 426 of the VP2 gene, which determines the variants of CPV-2; in addition, four samples were chosen for complete sequencing of the VP2 gene to identify all possible mutations in the circulating strains in this region of the country.ResultsThe results revealed the presence of the three variants of CPV-2 with a prevalence of 57.1% (20/35) for CPV-2a, 8.5% (3/35) for CPV-2b, and 34.3% (12/35) for CPV-2c. In addition, complete sequencing of the VP2 gene showed amino acid substitutions in residues 87, 101, 139, 219, 297, 300, 305, 322, 324, 375, 386, 426, 440, and 514 of the three Ecuadorian variants when compared with the original CPV-2 sequence.ConclusionThis study describes the detection of CPV variants in the city of Quito, Ecuador. Variants of CPV-2 (2a, 2b, and 2c) have been reported in South America, and there are cases in Ecuador where CVP-2 is affecting even vaccinated puppies.

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Full-length genomic characterization and molecular evolution of canine parvovirus in China

Cited by 16 publications

References 29 publications

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

An epidemiological investigation of porcine circovirus 3 infection in dogs in the Guangxi Province from 2015 to 2017, China

Genome sequence characterization of canine parvoviruses prevalent in the Sichuan province of China

Molecular characterization of canine parvovirus variants (CPV-2a, CPV-2b, and CPV-2c) based on the VP2 gene in affected domestic dogs in Ecuador

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