Full-length transcriptome analysis reveals the differences between floral buds of recessive genic male-sterile line (RMS3185A) and fertile line (RMS3185B) of cabbage

Tian, Aimei; Zhang, Enhui; Cui, Zhuoyue

doi:10.1007/s00425-020-03542-8

Cited by 7 publications

(3 citation statements)

References 62 publications

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“…1 c). KEGG enrichment analysis revealed that the targets of DEmiRNAs were significantly enriched in several pathways that may be related to anther development, including ‘plant hormone signal transduction’, which plays an important regulatory role in plant anther development 31 , ‘endocytosis’, which is essential for the development of male reproductive organs in Arabidopsis 32 , ‘plant–pathogen interaction’, which plays a dominate role in recessive genic male sterility of cabbage 33 , ‘protein processing in the endoplasmic reticulum’, and ‘oxidative phosphorylation’, which were also significantly enriched in the comparative transcriptome analysis of tobacco sua -cytoplasmic male sterility, indicating that they may be involved in anther development 34 (Fig. 1 d).…”

Section: Resultsmentioning

confidence: 99%

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Shi

Pang

Liu

et al. 2022

Sci Rep

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Anther development is precisely regulated by a complex gene network, which is of great significance to plant breeding. However, the molecular mechanism of anther development in Chinese cabbage is unclear. Here, we identified microRNAs (miRNAs), mRNAs, long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) related to anther development in Chinese cabbage (Brassica campestris L. ssp. pekinensis) to construct competitive endogenous RNA (ceRNA) regulatory networks and provide valuable knowledge on anther development. Using whole-transcriptome sequencing, 9055, 585, 1344, and 165 differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs), lncRNAs (DElncRNAs), and circRNAs (DEcircRNAs) were identified, respectively, in the anthers of Chinese cabbage compared with those in samples of the vegetative mass of four true leaves. An anther-related ceRNA regulatory network was constructed using miRNA targeting relationships, and 450 pairs of ceRNA relationships, including 97 DEmiRNA–DEmRNA, 281 DEmiRNA–DElncRNA, and 23 DEmiRNA–DEcircRNA interactions, were obtained. We identified important genes and their interactions with lncRNAs, circRNAs, and miRNAs involved in microsporogenesis, tapetum and callose layer development, pollen wall formation, and anther dehiscence. We analyzed the promoter activity of six predominant anther expression genes, which were expressed specifically in the anthers of Arabidopsis thaliana, indicating that they may play an important role in anther development of Chinese cabbage. This study lays the foundation for further research on the molecular mechanisms of anther growth and development in Chinese cabbage.

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Section: Resultsmentioning

confidence: 99%

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Shi

Pang

Liu

et al. 2022

Sci Rep

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“…The CBP genes encode a class of important messengers that contribute to the regulation of numerous cellular processes. Previous studies have found that CPBs play a key role in pollen tube germination, growth, guidance, and sperm delivery by functioning as calcium-sensitive signal molecules ( Hepler et al, 2012 ; Tian et al, 2021 ). The expression of CAM 7 was upregulated during the pollen germination and pollen tube growth of Arabidopsis, implying its critical involvement in pollen germination and tube growth ( Wang et al, 2008 ).…”

Section: Discussionmentioning

confidence: 99%

Integrative Analyses of Transcriptomics and Metabolomics in Sex Differentiation of Mulberry Flowers

Liu

Zhu

et al. 2022

Front. Mol. Biosci.

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Sex determination and sex differentiation of plants are important physiological processes of plant development. Mulberry (Morus indica L.) is an important economic tree being cultivated in sericulture countries, and mulberry leaf is commonly used for sericulture. The transcriptomic and metabolomic differences between the staminate flowers (SFs) and pistillate flowers (PFs) of mulberry were investigated by RNA sequencing and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Overall, we uncovered 4,230 genes and 209 metabolites are significantly differentially expressed between the SFs and PFs of mulberry. The combined transcriptomic and metabolomic analysis revealed these differentially expressed genes (DEGs) and differentially expressed metabolites (DEMs) are involved in flavonoid biosynthesis, galactose metabolism, plant–pathogen interaction, and starch and sucrose metabolism, and these detected DEGs and DEMs may be associated with sex differentiation of mulberry through the regulation of the enrichment pathways, such as the MAPK pathway, flavonoid biosynthesis, galactose metabolism, plant–pathogen interaction, and starch and sucrose metabolism. This study will provide a rich source for the analysis of the molecular mechanism of mulberry sex differentiation processes.

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“…Our laboratory has been devoted to research on the molecular mechanism of cabbage pollen development for many years and has identified genes such as polygalacturonase BcMF2 and BcMF9, arabinogalactoprotein BcMF8 and BcMF18, and pectin lyase BcPLL9 and BcPLL10 to regulate cabbage pollen development (Huang et al, 2009a(Huang et al, , 2009bJiang et al, 2014aJiang et al, , 2014bLim et al, 2013;Lin et al, 2018). Our previous research revealed that the expression level of the Bra015092 gene in the flower buds of the recessive male sterile line (RMS3185A) of Chinese cabbage was significantly higher than that of the fertile line (RMS3185B) using full-length transcriptome analysis (Tian et al, 2021). Protein structure and function prediction revealed that the Bra015092 gene encodes a RING-type E3 ubiquity ligase.…”

Section: Introductionmentioning

confidence: 99%

Functional characterization of E3 ubiquity ligase Bra015092 in pollen development of Brassica campestris ssp. Chinensis

Tian

Cui

2022

Physiologia Plantarum

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Pollen development plays an important role in the sexual reproduction of seed‐type plants. Ubiquitination of proteins is an essential link in the post‐translational modification of proteins. E3 ubiquity ligase is a key protein that recognizes substrates in the protein ubiquitination pathway. The hybrid line “Bcajh97‐01A/B” of Chinese cabbage (Brassica campestris L. ssp. Chinensis) was used as test material. The gene Bra015092, with a size of 642 bp, was amplified. Semi‐quantitative (RT‐PCR) and quantitative real‐time PCR (qRT‐PCR) techniques were utilized to analyze the expression of Bra015092 in the dual‐purpose line of Chinese cabbage. It was found that Bra015092 had a higher expression level in inflorescence. Subcellular localization analysis showed that Bra015092 and GFP fusion expression protein widely exist in tobacco epidermal cells. Bra015092 was transformed into “Youqing49” cabbage to obtain Bra015092OE overexpressing transgenic lines. The morphological observation of Bra015092OE plants showed that the pollen of BcMF29OE plants became deformed and inactive, and the vegetative and reproductive nuclei were abnormally developed. The in vitro germination experiments showed that about 24.5% of the pollen in Bra015092OE plants could not germinate. The results of the semi‐thin section showed that the pollen development of Bra015092OE plants was abnormal at the stage of binuclear pollen grains. Transmission electron microscopy revealed that the pollen grains of Bra015092OE plants gradually degraded from the binuclear to the trinucleate pollen grain stage, and the pollen inner wall was abnormally developed, indicating that Bra015092 plays a major role in the process of pollen development.

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Full-length transcriptome analysis reveals the differences between floral buds of recessive genic male-sterile line (RMS3185A) and fertile line (RMS3185B) of cabbage

Cited by 7 publications

References 62 publications

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Whole-transcriptome analysis and construction of an anther development-related ceRNA network in Chinese cabbage (Brassica campestris L. ssp. pekinensis)

Integrative Analyses of Transcriptomics and Metabolomics in Sex Differentiation of Mulberry Flowers

Functional characterization of E3 ubiquity ligase Bra015092 in pollen development of Brassica campestris ssp. Chinensis

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