Fully automatic d-lactate assay using a modified commercially available method

Validation of a short turnaround time automated method for the 24/7 determination of plasma d-lactate on Roche Cobas c502

“…Interference did not exceed 10% of d -lactate increase until a L-LDH level of 1000 IU.L −1 , which is consistent with the literature. To be noted that to avoid such interference, it is possible to deproteinize samples with precipitation or ultrafiltration [ 14 , 19 ]. Nevertheless, it implies additional preanalytical steps which can be challenging in routine practice.…”

Acute Intestinal Necrosis: The Preoperative Diagnostic Approach: Particularly References to Acute Vascular Intestinal Necrosis

“…Great expectations have been placed on D-lactate as an AIN biomarker. Thus, considerable effort was spent on the operationalization of Dlactate analysis in a fully automated spectrophotometric setup (172). The analysis was performed according to Rasmussen et al (172).…”

“…Thus, considerable effort was spent on the operationalization of Dlactate analysis in a fully automated spectrophotometric setup (172). The analysis was performed according to Rasmussen et al (172). Initial analytical considerations were built on cut-off levels in the literature as mentioned.…”

“…Arteriosclerosis in the IMA was reported in three out of four AIN patients in our study. A considerable effort was spent in the development of the D-lactate analysis, making it operational on an automated analytical setup ensuring fast and reliable analysis reports around the clock (172). Nevertheless, half of the patient cohort in this study was excluded from the analysis due to lipemic interference, and D-lactate failed to distinguish AIN patients from non-AIN patients in the nonexcluded part of the patients.…”