Fully Electronic Urine Dipstick Probe for Combinatorial Detection of Inflammatory Biomarkers

Kamakoti, Vikramshankar; Kinnamon, David; Choi, Kyoung-Hee; Jagannath, Badrinath; Prasad, Shalini

doi:10.4155/fsoa-2017-0142

Cited by 12 publications

(12 citation statements)

References 43 publications

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“…Electrochemical methods [14] are highly sensitive with easy implementation, and they are a candidate for miniaturization. Our group explored the electrochemical platform to fabricate a biosensor for important protein markers including CRP [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Design and Electrochemical Characterization of Spiral Electrochemical Notification Coupled Electrode (SENCE) Platform for Biosensing Application

et al. 2020

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C-reactive protein (CRP) is considered to be an important biomarker associated with many diseases. During any physiological inflammation, the level of CRP reaches its peak at 48 h, whereas its half-life is around 19 h. Hence, the detection of low-level CRP is an important task for the prognostic management of diseases like cancer, stress, metabolic disorders, cardiovascular diseases, and so on. There are various techniques available in the market to detect low-level CRP like ELISA, Western blot, etc. An electrochemical biosensor is one of the important miniaturized platforms which provides sensitivity along with ease of operation. The most important element of an electrochemical biosensor platform is the electrode which, upon functionalization with a probe, captures the selective antibody–antigen interaction and produces a digital signal in the form of potential/current. Optimization of the electrode design can increase the sensitivity of the sensor by 5–10-fold. Herein, we come up with a new sensor design called the spiral electrochemical notification coupled electrode (SENCE) where the working electrode (WE) is concentric in nature, which shows better response than the market-available standard screen-printed electrode. The sensor is thoroughly characterized using a standard Ferro/Ferri couple. The sensing performance of the fabricated platform is also characterized by the detection of standard H2O2 using a diffusion-driven technique, and a low detection limit of 15 µM was achieved. Furthermore, we utilized the platform to detect a low level (100 ng/mL) of CRP in synthetic sweat. The manuscript provides emphasis on the design of a sensor that can offer good sensitivity in electrochemical biosensing applications.

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Section: Introductionmentioning

confidence: 99%

Design and Electrochemical Characterization of Spiral Electrochemical Notification Coupled Electrode (SENCE) Platform for Biosensing Application

et al. 2020

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“…Simultaneous multiplex detection of urinary pathogens (bacterial nucleic acid (16S rRNA)) and lactoferrin by an amperometric immunosensor was demonstrated as a more promising and informative tool for diagnosis of the urinary tract infection than the current standard method [ 111 ]. Impedimetric quantification of C-reactive protein and interleukin 6 (IL6) by a novel portable dipstick-type immunosensor, based on molybdenum electrodes on nanoporous polyamide substrate, was reported in [ 112 ]; both proteins were detected in human urine samples with a LOD of 1 pg/mL. The ionic composition of urine can be useful for evaluating a patient’s general condition and diagnosing certain metabolic diseases [ 113 ].…”

Section: Application Of Msss and Msasmentioning

confidence: 99%

Multisensor Systems and Arrays for Medical Applications Employing Naturally-Occurring Compounds and Materials

Pauliukaitė

Voitechovič

2020

Sensors

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The significant improvement of quality of life achieved over the last decades has stimulated the development of new approaches in medicine to take into account the personal needs of each patient. Precision medicine, providing healthcare customization, opens new horizons in the diagnosis, treatment and prevention of numerous diseases. As a consequence, there is a growing demand for novel analytical devices and methods capable of addressing the challenges of precision medicine. For example, various types of sensors or their arrays are highly suitable for simultaneous monitoring of multiple analytes in complex biological media in order to obtain more information about the health status of a patient or to follow the treatment process. Besides, the development of sustainable sensors based on natural chemicals allows reducing their environmental impact. This review is concerned with the application of such analytical platforms in various areas of medicine: analysis of body fluids, wearable sensors, drug manufacturing and screening. The importance and role of naturally-occurring compounds in the development of electrochemical multisensor systems and arrays are discussed.

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“…It takes around five minutes for sensitive and specific detection of albumin in urine with a limit-of-detection (LoD) of 5 to 10 μg/ml, which is three times lower than the clinically accepted normal range. Beyond these colorimetric and fluorescent detection methods, other platforms commercially available and actively in research include an electrochemical urine dipstick probe biosensor that used molybdenum electrodes on nanoporous polyamide substrate for the quantitative multiplexed detection of inflammatory factors C-reactive protein (CRP) and Interleukin-6 (IL-6) in urine [74]. The portable electronics platform demonstrated results within five minutes with a LoD of 1 pg/ml for both CRP and IL-6 in human and synthetic urine.…”

Section: Protein Urinalysismentioning

confidence: 99%

“…As a result, fluids can be transported and manipulated through horizontal and vertical patterns tailored to the complexity and requirements of the given analytical application. An example of a 2D μPAD is explained in [74], as mentioned above.…”

Section: Microfluidic Paper-based Analytical Device (μPad) Applicationsmentioning

confidence: 99%

Current and emerging trends in point-of-care urinalysis tests

Lei

Huo

Mohan

2019

Expert Review of Molecular Diagnostics

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Introduction-The development of point-of-care testing (POCT) has made clinical diagnostics available, affordable, rapid, and easy to use since the 1990s. The significance of this platform rests on its potential to empower patients to monitor their own health status more frequently in the convenience of their home so that diseases can be diagnosed at the earliest possible time-point. Recent advances have expanded traditional formats such as qualitative or semi-quantitative dipsticks and lateral flow immunoassays to newer platforms such as microfluidics and paper-based assays where signals can be measured quantitatively using handheld devices.Areas Covered-This review discusses: (1) working principles and operating mechanisms of both existing and emerging POCT platforms, (2) urine analytes measured using POCT in comparison to the laboratory or clinical "gold standard," and (3) limitations of existing POCT and expectations of emerging POCT in urinalysis.Expert Opinion-Currently, a variety of biological samples such as urine, saliva, serum, plasma, and other fluids can be applied to POCT for quick diagnosis, especially in resource-limited settings. Emerging platforms will increasingly empower individuals to monitor their health status through frequent urine analysis even from their homes. The impact of these emerging technologies on healthcare is likely to be transformative.

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Fully Electronic Urine Dipstick Probe for Combinatorial Detection of Inflammatory Biomarkers

Cited by 12 publications

References 43 publications

Design and Electrochemical Characterization of Spiral Electrochemical Notification Coupled Electrode (SENCE) Platform for Biosensing Application

Design and Electrochemical Characterization of Spiral Electrochemical Notification Coupled Electrode (SENCE) Platform for Biosensing Application

Multisensor Systems and Arrays for Medical Applications Employing Naturally-Occurring Compounds and Materials

Current and emerging trends in point-of-care urinalysis tests

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