Fully integrated digital microfluidics platform for automated immunoassay; A versatile tool for rapid, specific detection of a wide range of pathogens

Coudron, Loïc; McDonnell, Martin B.; Munro, Ian; McCluskey, Daniel; Johnston, I. D. A.; Tan, Christabel; Tracey, M.C.

doi:10.1016/j.bios.2018.12.014

Cited by 69 publications

(50 citation statements)

References 50 publications

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“…The concept of such a device was invented in the late 1990s, employing various mechanisms of the actuation of droplets on the surface such as electro-wetting, surface accounting waves, magnetically controlled droplet movement, dielectrophoresis, or thermocapillary transport. There are numerous analytical applications of this device, for instance, for immunoassays of pathogens [278] in chemical synthesis, [279] or even in synthetic biology [280]. The developed processes with the movement of droplets and non-flow detection are considered to be discrete systems, not flow chemistry procedures; hence, they are not further discussed here.…”

Section: Microfluidics In Flow Analysis and Flow Synthesismentioning

confidence: 99%

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Trojanowicz

2020

Molecules

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Flow chemistry is an area of contemporary chemistry exploiting the hydrodynamic conditions of flowing liquids to provide particular environments for chemical reactions. These particular conditions of enhanced and strictly regulated transport of reagents, improved interface contacts, intensification of heat transfer, and safe operation with hazardous chemicals can be utilized in chemical synthesis, both for mechanization and automation of analytical procedures, and for the investigation of the kinetics of ultrafast reactions. Such methods are developed for more than half a century. In the field of chemical synthesis, they are used mostly in pharmaceutical chemistry for efficient syntheses of small amounts of active substances. In analytical chemistry, flow measuring systems are designed for environmental applications and industrial monitoring, as well as medical and pharmaceutical analysis, providing essential enhancement of the yield of analyses and precision of analytical determinations. The main concept of this review is to show the overlapping of development trends in the design of instrumentation and various ways of the utilization of specificity of chemical operations under flow conditions, especially for synthetic and analytical purposes, with a simultaneous presentation of the still rather limited correspondence between these two main areas of flow chemistry.

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Section: Microfluidics In Flow Analysis and Flow Synthesismentioning

confidence: 99%

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Trojanowicz

2020

Molecules

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“…In the portable DMF platform developed by Coudron et al an 18F45K22 microcontroller contains an 8-bit sine wave lookup table and output to an R-2R resistor ladder to produce a 1 kHz analog sine wave. After amplification, up to 225 V rms have been applied to 48 driving electrodes individually by a digital potentiometric control and use of high voltage MOSFET switches [48].…”

Section: Plos Onementioning

confidence: 99%

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

et al. 2020

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Electrowetting-on-dielectric is a decent technique to manipulate discrete volumes of liquid in form of droplets. In the last decade, electrowetting-on-dielectric systems, also called digital microfluidic systems, became more frequently used for a variety of applications because of their high flexibility and reconfigurability. Thus, one design can be adapted to different assays by only reprogramming. However, this flexibility can only be useful if the entire system is portable and easy to use. This paper presents the development of a portable, standalone digital microfluidic system based on a Linux-based operating system running on a Raspberry Pi, which is unique. We present "PortaDrop" exhibiting the following key features: (1) an "all-in-one box" approach, (2) a user-friendly, self-explaining graphical user interface and easy handling, (3) the ability of integrated electrochemical measurements, (4) the ease to implement additional lab equipment via Universal Serial Bus and the General Purpose Interface Bus as well as (5) a standardized experiment documentation. We propose that PortaDrop can be used to carry out experiments in different applications, where small sample volumes in the nanoliter to picoliter range need to be handled an analyzed automatically. As a first application, we present a protocol, where a droplet is consequently exchanged by droplets of another medium using passive dispensing. The exchange is monitored by electrical impedance spectroscopy. It is the first time, the media exchange caused by passive dispensing is characterized by electrochemical impedance spectroscopy. Summarizing, PortaDrop allows easy combination of fluid handling by means of electrowetting and additional sensing.

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“…NOTE: The photodetection system pivots around a photodiode, which has a large collection area (10 x 10 mm 2 ) to maximize light collection without additional optics, plus a trans-impedance amplifier to minimize noise 6 . However, the system is extremely sensitive and can collect minute signals.…”

Section: Loading Assembly and Operation Of The Ewod Chip On The Dmf mentioning

confidence: 99%

“…A detection technology capable of addressing the threats from pathogens must feature high-throughput (e.g. short assay time), efficiency (low Limit of Detection -LoDand high sensitivity) and specificity (to the target pathogen type) for it to be functional 6 .…”

Section: Introductionmentioning

confidence: 99%

“…The worst-case scenario is when a critical amount of protein adsorbs thus rendering the device useless. To minimize the biofouling, various approaches have been investigated from minimizing the residence time of the droplet on the chip, through coatings 23 , to additives (i.e., surfactants or pluronic acid) into the biomaterial-laden droplets 6,22 . Hence an important aspect of the immunoassay assay on EWOD is to choose anti-biofouling strategies that are compatible with the specific protocol at hand.…”

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confidence: 99%

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Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

Dimov

McDonnell

Munro

et al. 2020

JoVE

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Electrowetting is the effect by which the contact angle of a droplet exposed to a surface charge is modified. Electrowetting-on-dielectric (EWOD) exploits the dielectric properties of thin insulator films to enhance the charge density and hence boost the electrowetting effect. The presence of charges results in an electrically induced spreading of the droplet which permits purposeful manipulation across a hydrophobic surface. Here, we demonstrate EWOD-based protocol for sample processing and detection of four categories of antigens, using an automated surface actuation platform, via two variations of an Enzyme-Linked Immunosorbent Assay (ELISA) methods. The ELISA is performed on magnetic beads with immobilized primary antibodies which can be selected to target a specific antigen. An antibody conjugated to HRP binds to the antigen and is mixed with H 2 O 2 /Luminol for quantification of the captured pathogens. Assay completion times of between 6 and 10 min were achieved, whilst minuscule volumes of reagents were utilized. Video Link The video component of this article can be found at https://www.jove.com/video/60489/ 15. Recently, in-field diagnostic against measles and rubella IgG has been demonstrated in remote Northwestern Kenya's

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Fully integrated digital microfluidics platform for automated immunoassay; A versatile tool for rapid, specific detection of a wide range of pathogens

Abstract: Accepted/In press). Fully integrated digital microfluidics platform for automated immunoassay; a versatile tool for rapid, specific detection of a wide range of pathogens.

Cited by 69 publications

References 50 publications

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

Flow Chemistry in Contemporary Chemical Sciences: A Real Variety of Its Applications

PortaDrop: A portable digital microfluidic platform providing versatile opportunities for Lab-On-A-Chip applications

Electrowetting-based Digital Microfluidics Platform for Automated Enzyme-linked Immunosorbent Assay

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