Fully Integrated Thermoplastic Genosensor for the Highly Sensitive Detection and Identification of Multi‐Drug‐Resistant Tuberculosis

Wang, Hong; Chen, Hui-Wen; Hupert, Mateusz L.; Chen, Pin Chuan; Datta, Proyag; Pittman, Tana L.; Goettert, Jost; Murphy, Michael C.; Williams, Diana L.; Barany, Francis; Soper, Steven A.

doi:10.1002/anie.201200732

Cited by 41 publications

(35 citation statements)

References 33 publications

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“…Recently, a fully integrated thermoplastic microfluidic device providing sample-in-result-out based technology was demonstrated to detect DR-TB from clinical sputum samples [61]. DR-TB identification was enabled by the detection of single nucleotide variations in rpoB gene frequently associated with rifampicin-resistant Mtb utilizing a PCR/ligase system [61].…”

Section: Recent Advances In Nano/microscale Technologies For Tb/drmentioning

confidence: 99%

“…DR-TB identification was enabled by the detection of single nucleotide variations in rpoB gene frequently associated with rifampicin-resistant Mtb utilizing a PCR/ligase system [61]. The main advantage of the system is the use of inexpensive fluidic cartridge to perform multistep reactions such as cell lysis, solid-phase micropillar DNA extraction, PCR/ligase reaction, and endpoint array fluorescence detection (Fig.…”

Section: Recent Advances In Nano/microscale Technologies For Tb/drmentioning

confidence: 99%

“…(Adapted with permission from ref [61]). A) Schematic of a fluidic cartridge integrated with sample and reagent inlets/outlets (1-6), membrane valves (v1-v6) and serpentine reaction chambers that perform DNA extraction, PCR amplification, ligase detection reaction, and endpoint array-based fluorescence detection.…”

Section: Figmentioning

confidence: 99%

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Emerging technologies for monitoring drug-resistant tuberculosis at the point-of-care

Mani

Wang

İnci

et al. 2014

Advanced Drug Delivery Reviews

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Infectious diseases are the leading cause of death worldwide. Among them, tuberculosis (TB) remains a major threat to public health, exacerbated by the emergence of multiple drug-resistant (MDR) and extensively drug-resistant (XDR) Mycobacterium tuberculosis (Mtb). MDR-Mtb strains are resistant to first-line anti-TB drugs such as isoniazid and rifampicin; whereas XDR-Mtb strains are resistant to additional drugs including at least to any fluoroquinolone and at least one of the second-line anti-TB injectable drugs such as kanamycin, capreomycin, or amikacin. Clinically, these strains have significantly impacted the management of TB in high-incidence developing countries, where systemic surveillance of TB drug resistance is lacking. For effective management of TB on-site, early detection of drug resistance is critical to initiate treatment, to reduce mortality, and to thwart drug-resistant TB transmission. In this review, we discuss the diagnostic challenges to detect drug-resistant TB at the point-of-care (POC). Moreover, we present the latest advances in nano/microscale technologies that can potentially detect TB drug resistance to improve on-site patient care.

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Section: Recent Advances In Nano/microscale Technologies For Tb/drmentioning

confidence: 99%

Section: Recent Advances In Nano/microscale Technologies For Tb/drmentioning

confidence: 99%

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Emerging technologies for monitoring drug-resistant tuberculosis at the point-of-care

Mani

Wang

İnci

et al. 2014

Advanced Drug Delivery Reviews

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“…4,5 One of the most promising techniques for detecting point mutations is the polymerase chain reaction (PCR)/ligase detection reaction (LDR)/hybridization assay. [6][7][8][9][10][11][12][13][14][15] A conceptual schematic of this assay is depicted in Fig. 1.…”

Section: Introductionmentioning

confidence: 99%

Development of a PCR/LDR/Flow-Through Hybridization Assay Using a Capillary Tube, Probe DNA-Immobilized Magnetic Beads and Chemiluminescence Detection

et al. 2013

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A polymerase chain reaction (PCR)/ligase detection reaction (LDR)/flow-through hybridization assay using chemiluminescence (CL) detection was developed for analyzing point mutations in gene fragments with high diagnostic value for colorectal cancers. A flow-through hybridization format using a capillary tube, in which probe DNA-immobilized magnetic beads were packed, provided accelerated hybridization kinetics of target DNA (i.e. LDR product) to the probe DNA. Simple fluid manipulations enabled both allele-specific hybridization and the removal of non-specifically bound DNA in the wash step. Furthermore, the use of CL detection greatly simplified the detection scheme, since CL does not require a light source for excitation of the fluorescent dye tags on the LDR products. Preliminary results demonstrated that this analytical system could detect both homozygous and heterozygous mutations, without the expensive instrumentation and cumbersome procedures required by conventional DNA microarray-based methods.

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“…17–20 Building on this premise, several groups have reported polymer-based systems that can analyze genetic material. For example, a monolithic system, which integrated PCR and DNA microarrays, was described by Liu et al 21 The chip was fabricated in PC using CO 2 laser machining with the system employed for the analysis of both E. coli and Enterococcus faecalis .…”

Section: Introductionmentioning

confidence: 99%

Modular microfluidic system fabricated in thermoplastics for the strain-specific detection of bacterial pathogens

et al. 2012

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Fully Integrated Thermoplastic Genosensor for the Highly Sensitive Detection and Identification of Multi‐Drug‐Resistant Tuberculosis

Cited by 41 publications

References 33 publications

Emerging technologies for monitoring drug-resistant tuberculosis at the point-of-care

Emerging technologies for monitoring drug-resistant tuberculosis at the point-of-care

Development of a PCR/LDR/Flow-Through Hybridization Assay Using a Capillary Tube, Probe DNA-Immobilized Magnetic Beads and Chemiluminescence Detection

Modular microfluidic system fabricated in thermoplastics for the strain-specific detection of bacterial pathogens

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