Function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of Chromatium vinosum as studied with flash spectroscopy

Grondelle, Rienk van; Duysens, L.N.M.; wel, Jaap A. Van der; Wal, H. N. Van Der

doi:10.1016/0005-2728(77)90169-4

Cited by 91 publications

(23 citation statements)

References 28 publications

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“…The periplasmic cytochrome c-551 of which the complete purification has been described here for the first time is the fourth soluble cytochrome c, after a cytochrome c' (Bartsch, 1991), a low redox potential cytochrome c and a low spin/ high spin cytochrome c , isolated from the purple phototrophic bacterium C. vinosum. Evidence has previously shown that in whole cells of C. vinosum, a soluble ctype cytochrome c-551 would fulfill a role as electron carrier between the final donor pool and the oxidised membrane-bound c-type cytochromes of the photosynthetic reaction center (Van Grondelle et al, 1977). Our sequence data now reveal that the purified cytochrome c-551 belongs to the family of cytochromes c8.…”

Section: Discussionsupporting

confidence: 50%

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads

Samyn

Smet

Driessche

et al. 1996

European Journal of Biochemistry

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A minor cytochrome c-551 component of Chromatium vinosum was previously found to efficiently couple electron transfer between the cytochrome bc, complex and the photosynthetic reaction center. We have now determined the amino acid sequence of this cytochrome c-551 and find that it is homologous to cytochrome cx (formerly called Pseudomonas cytochrome c-551). It is most similar to Methylophilus methylotrophus, Rhodocyclus tenuis, and Azotobacter vinelandii cytochromes c, (respectively, 57 %, 52 % and 51%). The C. vinosum cytochrome c, has a single residue insertion relative to Pseudomonas and Azotobacter cytochromes cx. It has fewer charged residues than its homologs and is essentially neutral, which may explain why it is less soluble than the others. The cytochromes cx are only very distantly related to the cytochromes c2 found in other species of purple bacteria which are much larger in size and which usually mediate electron transfer between the cytochrome be, complex and the reaction center. The photosynthetic pathway in Chromatium thus appears to be radically different from that in purple non-sulfur bacteria.

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Section: Discussionsupporting

confidence: 50%

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads

Samyn

Smet

Driessche

et al. 1996

European Journal of Biochemistry

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“…This is also supported by the observation that photooxidized cytochrome c-555 accepts electrons from cytochrome c-551 which is located in the aqueous periplasmic space [9,22], and therefore cytochrome c-555 exposes at least its functional surface to the aqueous periplasmic space.…”

Section: Discussionsupporting

confidence: 61%

Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum

1983

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A membrane-bound cytochrome c-552 was isolated and purified from the photosynthetic bacterium Chromatium vinosum by treatment with sodium cholate, sodium deoxycholate and bacterial alkaline protease followed by gel filtration.The purified cytochrome c-552, which may have been modified by the protease treatment, was electrophoretically homogeneous. Its minimal molecular weight was estimated to be 19 and 20 kdaltons, respectively by SDS polyacrylamide gel electrophoresis and by gel filtration on Sephadex G-100.Cytochrome c-552 showed the absorption maxima at 419, 523 and 552 nm in the reduced form. Reduced-minus-oxidized difference millimolar absorption coefficient was 10.6 for the wavelength pair, 552 minus 540 nm. The midpoint potential at pH 8.0 was -130 mV. The polarity in the amino acid composition of cytochrome c-552 was 40.1% and reflected its hydrophobicity.The solubilized cytochrome c-552 was shown to be a different entity from the soluble flavocytochrome c-552 in several respects.

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“…rubrum and Rps. sphaeroides is difficult because in this case the electron is transferred from soluble c-551 to bound cytochrome c-555, but from the observed reaction rate [13] it may be surmised that the reaction probability is likewise small. In all these systems, despite the low reaction probability, the oxidation of soluble cytochrome is still sufficiently fast under physiological conditions to compete efficiently with the rate-limiting steps in the ubiquinol cytochrome c2 oxidoreductase which is in the order of 10 ms.…”

Section: Discussionmentioning

confidence: 99%

Oxidation of cytochrome c 2 by photosynthetic reaction centers of Rhodospirillum rubrum and Rhodopseudomonas sphaeroides in vivo. Effect of viscosity on the rate of reaction

1986

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Function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of Chromatium vinosum as studied with flash spectroscopy

Cited by 91 publications

References 28 publications

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads

Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum

Oxidation of cytochrome c 2 by photosynthetic reaction centers of Rhodospirillum rubrum and Rhodopseudomonas sphaeroides in vivo. Effect of viscosity on the rate of reaction

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Function and properties of a soluble c-type cytochrome c-551 in secondary photosynthetic electron transport in whole cells of Chromatium vinosum as studied with flash spectroscopy

Cited by 91 publications

References 28 publications

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C8 from Denitrifying Pseudomonads

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C8 from Denitrifying Pseudomonads

Isolation and properties of membrane-bound cytochrome c-552 from photosynthetic bacterium Chromatium vinosum

Oxidation of cytochrome c 2 by photosynthetic reaction centers of Rhodospirillum rubrum and Rhodopseudomonas sphaeroides in vivo. Effect of viscosity on the rate of reaction

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A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads

A High‐Potential Soluble Cytochrome C ‐551 from the Purple Phototrophic Bacterium Chromatium vinosum is Homologous to Cytochrome C₈ from Denitrifying Pseudomonads