Function of the Exon 7 Deletion Variant Estrogen Receptor α Protein in an Estradiol-Resistant, Tamoxifen-Sensitive Human Endometrial Adenocarcinoma Grown in Nude Mice

Transcriptional Interaction of an Estrogen Receptor Splice Variant and ErbB4 Suggests Convergence in Gene Susceptibility Pathways in Schizophrenia

“…This notion is based on a 30-fold higher expression of the ⌬3-ER␣ variant in normal breast cells rather than in breast cancer tissues (62,63). Moreover, estradiol treatment of human endometrial adenocarcinomas grown in nude mice show decreased WT-ER␣ and increased ⌬7-ER␣ protein expression (64). Our finding that SHSY5Y cells are capable of robust induction of estrogen-stimulated luciferase reporter activity by exogenous WT-ER␣ overexpression is consistent with this notion.…”

Observations on the presence of E domain variants of estrogen receptor‐α in the breast tumors

“…Most of the cases with metastasis and all the cases with recurrence were ER-negative as measured by ELISA. It is possible that in such cases the increased expression of varE5 and varE6 þ 7 following tamoxifen treatment generates a receptor variant that is non-functional and contributes to non-responsiveness to treatment as shown in endometrial cancer [40]. Like the findings of Chappel et al [28] and Desai et al [41], we also did not find any correlation between the expression of E domain variants and different stages of tumor progression.…”

Estrogen receptor (ER) expression and function in the pregnant human myometrium: estradiol via ERα activates ERK1/2 signaling in term myometrium

“…We performed further testing of the specificity of ERa immunodetection, since truncated ERa isoforms of various sizes have been reported previously in a number of human cells and tissues following the use of the ERa F10 and HC-20 antibodies (Flouriot et al 2000, Russell et al 2000, Horvath et al 2002, Figtree et al 2003, Penot et al 2005. By RNA interference, we reduced the expression of endogenous or overexpressed ERa protein in hTERT-HM myometrial smooth muscle cells, followed by ERa immunoblotting (Fig.…”