Function of the macrophage‑capping protein in colorectal carcinoma

Abstract

Abstract.To investigate the role of macrophage-capping protein (CapG) in the development and progression of colorectal carcinoma (CRC), immunohistochemistry (IHC), Kaplan-Meier survival analysis, wound healing and Transwell migration assays were performed. The IHC results demonstrated that CapG was relatively highly expressed in CRC tissue compared with non-tumor tissue (P<0.001), and that the expression of CapG was significantly associated with the tumor site, differentiation, lymph node metastasis and clinical stage (P=0.021, P=0.036, P=0.012 and P=0.009, respectively). Wound healing and Transwell migration assays demonstrated that the reduction of CapG expression in a CRC cell line by RNA interference was associated with significantly impaired motility (P<0.001). Kaplan-Meier survival analysis revealed that the expression of CapG in tumor samples was not significantly associated with disease-free survival time. In conclusion, CapG was overexpressed in CRC and was associated with tumor progression; therefore, it may be a useful prognostic biomarker and therapeutic target in CRC.

Multidirectional Pharma-Toxicological Study on Harpagophytum procumbens DC. ex Meisn.: An IBD-Focused Investigation

“…Of note are the pro-homeostatic effect on the levels of peroxiredoxin-2 (PRDX2), glutathione reductase (GSHR), catalase (CATA), and superoxide dismutase (SODC), which are deeply involved in contrasting oxidative stress-induced organ injury [40,41]. In addition, the water extract of H. procumbens normalized the levels of specific proteins, namely, drebrin-like protein (DBNL), macrophage-capping protein (CAPG), prothymosin alpha (PTMA), and high mobility group protein B2 (HGMB2), which are involved in the anti-proliferative effect, T-cell regulation, and defense against opportunistic infections [42][43][44][45]. Collectively, the pro-homeostatic effect exerted by the extract on the selected proteins is consistent with the reported anti-radical/anti-oxidant, anti-inflammatory, and anti-micotic effects.…”

“…Collectively, the pro-homeostatic effect exerted by the extract on the selected proteins is consistent with the reported anti-radical/anti-oxidant, anti-inflammatory, and anti-micotic effects. Moreover, considering the involvement of CAPG in the colon cancer progression [45], the present proteomic analysis further corroborates the anti-proliferative effect exerted by H. procumbens water extract (1000 µg/mL) on the human colon cancer HCT116 cell line [8]. On the other hand, the tested extract was ineffective against a limited number (n = 4) of proteins, whereas it determined a supra-physiological alteration of about 30 proteins, whose levels were not modified by the LPS stimulus.…”

Cross-Species Proteomics Identifies CAPG and SBP1 as Crucial Invasiveness Biomarkers in Rat and Human Malignant Mesothelioma

“…Its upregulation in clinical high-grade glioblastoma has also been reported by Xing and Zeng [ 25 ], while the correlation of its expression level with shorter survival time was demonstrated by Fu et al [ 26 ]. Moreover, the link between its abundance and occurrence of lymph node metastasis has also been documented for three different types of cancer [ 20 , 27 , 28 ], as well as its association with the prediction of response to treatment [ 20 , 29 ].…”

Proteomic analysis of nasal mucus samples of healthy patients and patients with chronic rhinosinusitis

“…Macrophage-capping protein (CAPG) is an actin-binding protein of the gelsolin/villin superfamily associated with cell motility. 41 CAPG has exhibited its importance in host immunity through macrophage motility and facilitation of IgG complement phagocytosis. 42 Thus, the reduced expression in CRSsNP mucus observed in this study may indicate impaired macrophage mediated inflammatory responses.…”