Function of the Polyketide Synthase Domains of <i>Schizochytrium</i> sp. on Fatty Acid Synthesis in <i>Yarrowia lipolytica</i>

Jia, Yu-Lei; Du, Fei; Nong, Fang-Tong; Li, Jin; Huang, Pengwei; Ma, Wang; Gu, Yang; Sun, Xiao‐Man

doi:10.1021/acs.jafc.2c08383

Cited by 10 publications

(2 citation statements)

References 48 publications

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“…is incomplete [31,32]. The PKS pathway requires less NADPH and produces fewer intermediate fatty acids than the ELO/DES pathway [33]. The polyketide synthase gene cluster of Schizochytrium sp.…”

Section: Polyketide Synthase (Pks) Pathwaymentioning

confidence: 99%

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Song,

Wang,

Chen

et al. 2024

Marine Drugs

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Microalgal lipids hold significant potential for the production of biodiesel and dietary supplements. To enhance their cost-effectiveness and commercial competitiveness, it is imperative to improve microalgal lipid productivity. Metabolic engineering that targets the key enzymes of the fatty acid synthesis pathway, along with transcription factor engineering, are effective strategies for improving lipid productivity in microalgae. This review provides a summary of the advancements made in the past 5 years in engineering the fatty acid biosynthetic pathway in eukaryotic microalgae. Furthermore, this review offers insights into transcriptional regulatory mechanisms and transcription factor engineering aimed at enhancing lipid production in eukaryotic microalgae. Finally, the review discusses the challenges and future perspectives associated with utilizing microalgae for the efficient production of lipids.

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Section: Polyketide Synthase (Pks) Pathwaymentioning

confidence: 99%

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Song,

Wang,

Chen

et al. 2024

Marine Drugs

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“…Heterologous expression of orfABC genes of Schizochytrium sp. in E. coli or Yarrowia lipolytica led to accumulation of long-chain PUFAs in resulting recombinants [ 6 , 12 ]. Overexpression of the KS domain of ORFA subunit from Thraustochytrium sp.…”

Section: Introductionmentioning

confidence: 99%

Selectively superior production of docosahexaenoic acid in Schizochytrium sp. through engineering the fatty acid biosynthetic pathways

Liu,

Han,

Chen

et al. 2024

Biotechnol Biofuels

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Background Schizochytrium sp. is commercially used for production of docosahexaenoic acid (DHA). Schizochytrium sp. utilizes the polyketide synthase complex (PKS) and a single type I fatty acid synthase (FAS) to synthesize polyunsaturated fatty acids and saturated fatty acids, respectively. The acyl carrier protein (ACP) domains of FAS or PKS are used to load acyl groups during fatty acids biosynthesis. Phosphopantetheinyl transferase (PPTase) transfers the pantetheine moiety from Coenzyme A to the conserved serine residue of an inactive ACP domain to produce its active form. Results In this study, in order to improve production and content of DHA, we decreased the expression of fas, strengthened the expression of the PKS pathway, and enhanced the supply of active ACP in Schizochytrium sp. ATCC20888. Weakening the expression of fas or disruption of orfA both led to growth defect and reduction of lipid yields in the resulting strains WFAS and DPKSA, indicating that both FAS and PKS were indispensable for growth and lipid accumulation. Although WFAS had a higher DHA content in total fatty acids than the wild-type strain (WT), its growth defect and low DHA yield hinders its use for DHA production. Overexpression of the orfAB, orfC, orfC-DH (truncated orfC), or ppt promoted DHA and lipid production, respectively. The yields and contents of DHA were further increased by combined overexpression of these genes. Highest values of DHA yield (7.2 g/L) and DHA content (40.6%) were achieved in a recombinant OPKSABC-PPT, ⁓56.5% and 15.3% higher than the WT values, respectively. Conclusions This study demonstrates that genetic engineering of the fatty acid biosynthetic pathways provides a new strategy to enhance DHA production in Schizochytrium.

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Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology

Shen,

Yan,

et al. 2023

Biotechnol Lett

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Function of the Polyketide Synthase Domains of Schizochytrium sp. on Fatty Acid Synthesis in Yarrowia lipolytica

Cited by 10 publications

References 48 publications

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Engineering Fatty Acid Biosynthesis in Microalgae: Recent Progress and Perspectives

Selectively superior production of docosahexaenoic acid in Schizochytrium sp. through engineering the fatty acid biosynthetic pathways

Expansion of YALIcloneHR toolkit for Yarrowia lipolytica combined with Golden Gate and CRISPR technology

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