Functional acellular matrix for tissue repair

Wang, Bin; Tang, Qinglai; Yang, Qian; Li, Mengmeng; Zeng, Shiying; Yang, Xinming; Xiao, Zhu; Tong, Xinying; Lei, Lanjie; Li, Shisheng

doi:10.1016/j.mtbio.2022.100530

Cited by 39 publications

(35 citation statements)

References 376 publications

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“…Recently, nanofibers development has made a major contribution to the scope of scaffolds for fabrication that can potentially meet this challenge in combination with an acellular scaffold. Regardless of their fabrication criteria, nanofibers have been used as a scaffold for several applications, e.g., musculoskeletal, heart valve, skin, neural, and vascular tissue engineering [ 11 , 12 , 13 ]. Tranquillo et al reported for the first time a tubular heart valve fabricated from two decellularized, engineered tissue tubes connected with absorbable sutures, which met this need, in principle [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment

et al. 2023

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Right ventricle outflow tract obstruction (RVOTO) is a congenital pathological condition that contributes to about 15% of congenital heart diseases. In most cases, the replacement of the right ventricle outflow in pediatric age requires subsequent pulmonary valve replacement in adulthood. The aim of this study was to investigate the extracellular matrix scaffold obtained by decellularization of the porcine pulmonary valve using a new detergent (Tergitol) instead of Triton X-100. The decellularized scaffold was evaluated for the integrity of its extracellular matrix (ECM) structure by testing for its biochemical and mechanical properties, and the cytotoxicity/cytocompatibility of decellularized tissue was assessed using bone marrow-derived mesenchymal stem cells. We concluded that Tergitol could remove the nuclear material efficiently while preserving the structural proteins of the matrix, but without an efficient removal of the alpha-gal antigenic epitope. Therefore, Tergitol can be used as an alternative detergent to replace the Triton X-100.

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Section: Introductionmentioning

confidence: 99%

Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment

et al. 2023

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“…In recent years, the acellular extracellular decellularized matrices of livers have been used to produce hydrogels. This material is then processed (undone in relation to the shape of the organ) and mixed with cells, to fabricate bioinks that are customary in bioprinting [ 31 ]. Thus, the protocol for decellularization described in the present paper can be used to manufacture optimized biomaterials.…”

Section: Discussionmentioning

confidence: 99%

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

2023

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Decellularization of organs creates an acellular scaffold, ideal for being repopulated by cells. In this work, a low-cost perfusion system was created to be used in the process of liver decellularization and as a bioreactor after recellularization. It consists of a glass chamber to house the organ coupled to a peristaltic pump to promote liquid flow through the organ vascular tree. The rats’ liver decellularization was made with a solution of sodium dodecyl sulfate. The recellularization was made with 108 mesenchymal stromal/stem cells and cultivated for seven days. The decellularized matrices showed an absence of DNA while preserving the collagen and glycosaminoglycans quantities, confirming the efficiency of the process. The functional analyses showed a rise in lactate dehydrogenase levels occurring in the first days of the cultivation, suggesting that there is cell death in this period, which stabilized on the seventh day. Histological analysis showed conservation of the collagen web and some groups of cells next to the vessels. It was possible to establish a system for decellularization and a bioreactor to use for the recellularization method. It is easy to assemble, can be ready to use in little time and be easily sterilized.

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“…Additionally, different species of NPs, such as polymers, gelatin, upconversion nanoparticles (UCNPs), iron oxide, black phosphorus (BP) nanosheets, gold, and mesoporous silica, have been successfully employed for CM camouflaging. [13,[25][26][27][28][29][30][31] To fabricate CM-NPs, it is necessary to acquire an adequate number of source cells from an appropriate source. The existing infrastructure for blood collection and processing makes it relatively straightforward to obtain primary blood cells from commercial sources.…”

Section: Sources Of Membrane Coatings and Nps Corementioning

confidence: 99%

Biomedical Applications of Cell Membrane‐Based Biomimetic Nano‐Delivery System

Zhang,

Wang,

et al. 2023

Advanced Therapeutics

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Surface modification of nanoparticles (NPs) is crucial for their effective utilization in diverse medical conditions, as it addresses challenges such as rapid passive clearance by the immune system, damage to the reticuloendothelial system, lack of specific cell targeting, and inadequate biocompatibility. The natural cell membrane (CM)‐coating technology has recently garnered considerable attention as an optimal strategy for enhancing artificial nanoformulations. This biomimetic technique directly confers inner NPs with multiple functions, such as immune evasion, precise targeting, and excellent biocompatibility, all of which are mediated by source cell‐inherent surface membrane proteins. Additionally, engineering techniques originally designed to enhance existing theranostic modalities employed in clinical settings are applied to natural CM carriers, thereby improving the physical properties of nanomaterials to drive further advancements. In this comprehensive review, diverse CM sources, such as red blood cells, platelets, white blood cells, cancer cells, bacteria, and other unconventional types, the core NPs employed in these cell membrane‐coated nanoparticles (CM‐NPs), preparation methods, and the latest advancements of CM‐NPs across diverse biomedical fields, are reviewed. Engineering strategies for enhancing natural CMs have also been emphasized. Furthermore, the review comprehensively addresses the major challenges and their corresponding solutions in the clinical translation of this evolving field.

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Functional acellular matrix for tissue repair

Cited by 39 publications

References 376 publications

Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment

Tergitol Based Decellularization Protocol Improves the Prerequisites for Pulmonary Xenografts: Characterization and Biocompatibility Assessment

Easy-to-Assembly System for Decellularization and Recellularization of Liver Grafts in a Bioreactor

Biomedical Applications of Cell Membrane‐Based Biomimetic Nano‐Delivery System

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