Functional Analysis of a Novel Chrysanthemum WRKY Transcription Factor Gene Involved in Salt Tolerance

Liu, Qinglin; Xu, Kedong; Pan, Yuanzhi; Jiang, Beibei; Liu, Guangli; Yin, Jing; Zhang, Haiqing

doi:10.1007/s11105-013-0639-3

Cited by 45 publications

(35 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, the antioxidant enzyme genes AtAPX, AtCAT, and AtGST which can scavenge reactive oxygen species (ROS) to protect plants from oxidative damage (Mittler et al 2004) are important downstream genes regulated by WRKY TFs. The increase of salt tolerance in plants may be due to the high expression of these genes (Doğan 2012;Shi et al 2014b;Liu et al 2014). In our study, the expression levels of AtAPX, AtCAT, and AtGST were downregulated, suppression of which could decrease tolerance to salt stress in the transgenic A. thaliana.…”

Section: Mrwrky30 Is Involved In Abiotic Resistancementioning

confidence: 48%

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Jiang

Zhang

et al. 2015

Protoplasma

View full text Add to dashboard Cite

WRKY transcription factors (TFs) play important roles in many plant processes, including responses to biotic and abiotic stresses. In the present study, Muscadinia rotundifolia MrWRKY30 dramatically accumulated in grapevine leaves in response to inoculation of Plasmopara viticola, a pathogen causing grapevine downy mildew disease. Similar responses were also found on grapevines treated with exogenous SA/JA/ET. Ectopic expression of MrWRKY30 in Arabidopsis thaliana "COL0" enhanced its resistance to downy mildew pathogen Peronospora parasitica. Pathogenesis-related (PR) genes, including AtPR1, AtPR4, AtPR5, and AtPDF1.2, were significantly upregulated in transgenic A. thaliana after P. parasitica inoculation. In the mean time, two critical genes in SA and JA signaling pathways, AtEDS5 and AtJAR1, were abundantly expressed as well, indicating that MrWRKY30 may enhance disease resistance of A. thaliana through SA and JA defense system. The transgenic A. thaliana plants also enhanced tolerance to cold stress accompanied with upregulation of AtCBF1, AtCBF3, AtICE1, and AtCOR47. MrWRKY30 might protect A. thaliana from cold damage by activating the AtCBF-mediated signaling pathway to induce the downstream AtCOR47 gene. Interestingly, the transgenic seedlings had a negative effect on salt tolerance. Reverse transcription PCR (RT-PCR) analysis revealed that antioxidant enzyme genes AtAPX (ascorbate peroxidase), AtCAT (catalase), and AtGST (glutathione-S-transferase) were suppressed in transgenic plants, which may lead to reactive oxygen species (ROS)-mediated sensitivity to salt stress.

show abstract

Section: Mrwrky30 Is Involved In Abiotic Resistancementioning

confidence: 48%

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Jiang

Zhang

et al. 2015

Protoplasma

View full text Add to dashboard Cite

show abstract

“…In salt stress conditions the expression of WRKY33 is more with 6.1 folds increase in the mRNA transcript levels and two genes WRKY 40 and WRKY 53 were found to be down-regulated. In earlier reports, the overexpression of DgWRKY1 from chrysanthemum has shown better tolerance to salt stress in transgenic tobacco plants [37].…”

Section: Discussionmentioning

confidence: 91%

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (<i>Macrotyloma uniflorum</i> (Lam.) Verdc.)

Kiranmai¹,

Gunupuru²,

Nareshkumar³

et al. 2016

AJMB

View full text Add to dashboard Cite

Drought and salt stress are two major environmental constraints that limit the productivity of agriculture crops worldwide. WRKY transcription factors are the plantspecific transcription factors that regulate several developmental events and stress responses in plants. The WRKY domain is defined by a 60-amino acid conserved sequence named WRKYGQK at N-terminal and a Zinc Finger-like motif at the Cterminal. WRKY genes are known to respond several stresses which may act as negative or positive regulators. The function of most of the WRKY transcription factors from non-model plants remains poorly understood. This investigation shows the expression levels of eight WRKY transcription factor genes from horsegram plant under drought and salt stress conditions. The increase in mRNA transcript levels of WRKY transcription factor genes was found to be high in drought stressed plants compared to salt-stressed plants. The levels of MDA which indicates the lipid peroxidation were less in drought stress. More ROS is produced in salt stress conditions compared to drought. The results show that the expression of WRKY transcription factors in drought stress conditions is reducing the adverse effect of stress on plants. These results also suggest that, during abiotic stress conditions such as drought and salt stress, WRKY transcription factors are regulated at the transcription level.

show abstract

“…Some other WRKY TFs were reported to localize also in the nucleus (Ramamoorthy et al 2008;Liu et al 2014;Wang et al 2016). The chlorophyl content and RWC were usually used as the markers for drought stress degree, which in BdWRKY36-overexpressing tobaccos were more higher than those in WT line under drought stress (Sun et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Studies on WRKY genes predominantly point to an involvement in salicylic acid signaling and disease responses (Eulgem et al 2000). Moreover, WRKY genes are involved in plant responses to drought, heat, cold, salinity (Shen et al 2012;Liu et al 2014;Wang et al 2016), and oxidative stress (Rizhsky et al 2004). Many WRKY TFs related to the drought stress responses have been isolated and studied in the recent years.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Han

Zhang

Ding

et al. 2018

Journal of Plant Interactions

View full text Add to dashboard Cite

Plant-specific WRKY transcription factors are widely involved in abiotic stress responses. In this study, a WRKY gene was isolated from Malus baccata (L.) Borkh and designated it as MbWRKY2. Subcellular localization showed that MbWRKY2 was localized in the nucleus. The expression levels of MbWRKY2 were up-regulated by abiotic stresses in M. baccata. When MbWRKY2 was introduced into tobacco, it improved drought stress tolerance in transgenic plants. The transgenic tobaccos had the higher contents of chlorophyl, proline, relative water content, AsA, and GSH, increased activities of CAT, APX, SOD, and POD, and decreased levels of MDA, H 2 O 2 , and electrolyte leakage than wild-type, especially when dealt with dehydration treatment. Moreover, the MbWRKY2-OE plants enhanced the expression of oxidative stress response and stress-related genes involved in osmotic adjustment and membrane protection. These results suggest that MbWRKY2 gene plays a positive regulatory role in drought stress response. ARTICLE HISTORY

show abstract

Functional Analysis of a Novel Chrysanthemum WRKY Transcription Factor Gene Involved in Salt Tolerance

Cited by 45 publications

References 24 publications

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (<i>Macrotyloma uniflorum</i> (Lam.) Verdc.)

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Contact Info

Product

Resources

About

Functional Analysis of a Novel Chrysanthemum WRKY Transcription Factor Gene Involved in Salt Tolerance

Cited by 45 publications

References 24 publications

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Isolation of a WRKY30 gene from Muscadinia rotundifolia (Michx) and validation of its function under biotic and abiotic stresses

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (&lt;i&gt;Macrotyloma uniflorum&lt;/i&gt; (Lam.) Verdc.)

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Contact Info

Product

Resources

About

Expression Analysis of WRKY Transcription Factor Genes in Response to Abiotic Stresses in Horsegram (<i>Macrotyloma uniflorum</i> (Lam.) Verdc.)