Functional Analysis of a Novel Complement C5a Receptor 1-Blocking Monoclonal Antibody
Leon Cyranka,
Ida Mariegaard,
Mikkel-Ole Skjødt
et al.
Abstract:Introduction
The complement system anaphylatoxin C5a is a critical player in inflammation. By binding to complement C5a receptor 1 (C5aR1/CD88), C5a regulates many cellular functions, mainly as a potent pro-inflammatory inducer, such as cytokine release, cellular motility, and homeostasis. We describe the generation and selection of a potent antagonistic C5aR1 mouse monoclonal antibody (mAb).
Methods and results
Initial C5aR1 hybridoma clone selection was performed with a cell binding study on human whole blo… Show more
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