Functional analysis of a point mutation in the ryanodine receptor of Plutella xylostella (L.) associated with resistance to chlorantraniliprole

Abstract

For the first time we experimentally proved that the G4946E mutation in PxRyR confers resistance to chlorantraniliprole in Plutella xylostella. These findings pave the way for the complete understanding of the mechanisms of diamide insecticides resistance in insects.

Molecular characteristics, mRNA expression, and alternative splicing of a ryanodine receptor gene in the Oriental fruit fly,Bactrocera dorsalis(Hendel)

“…Additionally, a single amino acid substitution (G4946E) in the C-terminal membrane-spanning domain of the RyR was reported to be associated with high levels of diamide resistance in field P. xylostella populations collected from the Philippines and Thailand [30]. This was confirmed using ligand binding assays [32].…”

“…Additionally, an amino acid replacement (G4946E) in the C-terminal membrane-spanning domain of the PxRyR was associated with diamide cross-resistance in P. xylostella collected from the Philippines and Thailand [30]. Subsequently, it was experimentally proven that the G4946E mutation in PxRyR conferred resistance to chlorantraniliprole in P. xylostella [32].…”

Ryanodine receptor genes of the rice stem borer, Chilo suppressalis: Molecular cloning, alternative splicing and expression profiling

“…xylostella RyR [34], was found as well. Other typical residues (I 4987 , R 5003 and D 5007 ) [35], which play an important role in activity and conductance of the Ca 2+ release channel and were conserved in OcRyR, OfRyR and CmRyR, were totally observed in CsRyR.…”

Seasonal proportion change of ryanodine receptor mutation (G4946E) in diamondback moth populations

“…[5][6][7][8] The diamide resistance of P. xylostella has been shown to be incompletely or almost completely recessive. 7,9,10) The amino acid mutation (G4946E) in RyR has been shown to be a major factor related to target-insensitive diamide resistance in P. xylostella. 5,[9][10][11] A method to estimate the proportions of G4946E in P. xylostella populations has been developed using quantitative sequencing (QS).…”

“…7,9,10) The amino acid mutation (G4946E) in RyR has been shown to be a major factor related to target-insensitive diamide resistance in P. xylostella. 5,[9][10][11] A method to estimate the proportions of G4946E in P. xylostella populations has been developed using quantitative sequencing (QS). 12) Unlike conventional time-consuming bioassay methods, the QS-based method can estimate the proportion using insects collected in the field in a short time.…”