Functional analysis of cytochrome P450 genes linked with acetamiprid resistance in melon aphid, Aphis gossypii

Ullah, Farman; Gül, Hina; Tariq, Kaleem; Desneux, Nicolas; Gao, Xiwu; Song, Dunlun

doi:10.1016/j.pestbp.2020.104687

Cited by 61 publications

(49 citation statements)

References 58 publications

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“…IDGFs were confirmed to be the proteins cooperating with insulin that promote cell lineages derived from imaginal discs in Drosophila melanogaster (Kawamura et al, 1999;Varela et al, 2002;Zurovcová and Ayala, 2002). RNAi has been widely used to find out the functions of vital genes in different insects of economic importance (Tomoyasu and Denell, 2004;Chen et al, 2008;Gong et al, 2012;Asokan et al, 2013;Zhang et al, 2013;Qi et al, 2015;Wang et al, 2017;Ullah et al, 2020a). Recently, a study reported that silencing of IDGF6 in Bactrocera correcta through RNAi significantly decreases the expression of IDGF6, causes larval mortality and wing malformation in adult flies (Zhao et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly, Zeugodacus cucurbitae

et al. 2021

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This study reports the first successful use of oral feeding dsRNA technique for functional characterization of imaginal disc growth factors (IDGFs) genes (IDGF1, IDGF3_1, IDGF4_0, IDGF4_1, and IDGF6) in melon fly Zeugodacus cucurbitae. Phylogenetic and domain analysis indicates that these genes had high similarity with other Tephritidae fruit flies homolog and contain only one conserved domain among these five genes, which is glyco-18 domain (glyco-hydro-18 domain). Gene expression analysis at different developmental stages revealed that these genes were expressed at larval, pupal, and adult stages. To understand their role in different developmental stages, larvae were fed dsRNA-corresponding to each of the five IDGFs, in an artificial diet. RNAi-mediated knockdown of IDGF1 shows no phenotypic effects but caused mortality (10.4%), while IDGF4_0 caused malformed pharate at the adult stage where insects failed to shed their old cuticle and remained attached with their body, highest mortality (49.2%) was recorded compared to dsRNA-green fluorescent protein (GFP) or DEPC. Silencing of IDGF3_1 and IDGF4_1 cause lethal phenotype in larvae, (17.2%) and (40%) mortality was indexed in Z. cucurbitae. IDGF6 was mainly expressed in pupae and adult stages, and its silencing caused a malformation in adult wings. The developmental defects such as malformation in wings, larval–larval lethality, pupal–adult malformation, and small body size show that IDGFs are key developmental genes in the melon fly. Our results provide a baseline for the melon fly management and understanding of IDGFs specific functions in Z. cucurbitae.

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Section: Introductionmentioning

confidence: 99%

RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly, Zeugodacus cucurbitae

et al. 2021

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“…Target mutations and the enhancement of the metabolic activities of detoxification enzymes are the two primary factors responsible for insecticide resistance in insects (Heckel 2012;Li et al 2007;Liu et al 2017). Different detoxification enzymes such as P450, CarE and GSTs have been reported to contribute to resistance Liu 2017, 2019;Li et al 2018;Lumjuan et al 2011;Ullah et al 2020b;Wang et al 2013). In this study, five metabolism-related differential proteins were found, and they accounted for the highest proportion of the differentially-expressed proteins.…”

Section: Discussionmentioning

confidence: 54%

“…The metabolic resistance, target-site resistance, penetration and behavioral resistance are primary resistance mechanisms in the target insects (Guedes et al 2019;Ingham et al 2018). Previously it was reported that cytochrome P450 monooxygenases (P450s) genes was involved in acetamiprid resistance in A. gossypii and chlorantraniliprole resistance in P. xylostella (L.) (Li et al 2018;Ullah et al 2020b). P450 gene regulate the insecticide resistance of mosquitoes through GPCR/Gαs/AC/cAMP-PKA signaling pathways Liu 2017, 2019).…”

Section: Introductionmentioning

confidence: 99%

The glutathione S-transferase (PxGST2L) may contribute to the detoxification metabolism of chlorantraniliprole in Plutella xylostella(L.)

et al. 2021

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The diamondback moth (Plutella xylostella L.), is an economic pest of cruciferous plants worldwide, which causes great economic loss to cruciferous plants production. However, the pest has developed resistance to insecticides. One of such insecticides is chlorantraniliprole. The study of the mechanisms underlying resistance is key for the effective management of resistance. In this study, a comparative proteomics approach was used to isolate and identify various proteins that differed between chlorantraniliprole-susceptible and -resistant strains of P. xylostella. Eleven proteins were significantly different and were successfully identified by MALDI-TOF-MS. Metabolism-related proteins accounted for the highest proportion among the eleven different proteins. The function of the PxGST2L protein was validated by RNAi. Knockdown of PxGST2L reduced the GST activity and increased the toxicity of chlorantraniliprole to the diamondback moth. The resistance ratio of diamondback moth to chlorantraniliprole was reduced from 1029 to 505. The results indicated that PxGST2L is partly responsible for chlorantraniliprole insecticide resistance in DBM. Our finding contributes to the understanding of the mechanism underlying resistance to chlorantraniliprole in the DBM, to develop effective resistance management tactics.

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“…5). In addition, CYP450 is a multifunctional enzyme, which plays an important role in the formation of insecticide resistance [34]. In a variety of animal cells, it has been proved that a variety of insecticides can induce the production of reactive oxygen species (ROS), and then induce oxidative stress [35].…”

Section: Discussionmentioning

confidence: 99%

Sublethal Effects of Bifenazate on Biological Traits and Enzymatic properties in the Panonychus citri (Acari: Tetranychidae)

Wang

Xin

Wang

et al. 2021

Preprint

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Panonychus citri, is a major citrus pest with a global distribution. But in Pest Management, the extensive use of insecticides has resulted in insecticide tolerance. Bifenazate, a novel acaricides with high biological activity against red mites, such as the T.urticae, P. citri. However, its sublethal effects on P. citri have not been reported. Therefore, in present study, P. citri exposed to sublethal concentration of bifenazate shows high biological activity, and causes great changes of population parameters and enzymatic activity. Life table technology was used to demonstrate sublethal effects on the growth and reproduction of P. citri. The results showed that the developmental duration of immature stage used sublethal concentration of bifenazate (LC10, LC30) were no difference than control. Nevertheless, compared with control group, the female adult duration, the fecundity and longevity were significantly decrease, but the pre-oviposition period was longer; In addition, the net reproductive rate (R0), the mean generation(T) were significantly decreased compared to those of the control, but the intrinsic rate of increase(r), the finite rate(λ) were decreased in LC30 group, however, the doubling time was increased with the increasing concentrations of bifenazate. Furthermore, Enzymatic tests showed that CAT, POD, and CarE activities were higher in LC10 and LC30 treatments than control. The SOD and GST activities were lower in LC30 treatment than control and LC10 treatment. Moreover, the CYP450 activity was decreased with the increasing concentrations of bifenazate. Based on these results, the sublethal concentration of bifenazate may have adverse effects on population growth of P. citri. This result provides certain significant guidance for the Pest Management. As well as that, further studies should be done to explore resistance mechanisms of P. citri exposure to a sublethal concentrations of bifenazate.

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Functional analysis of cytochrome P450 genes linked with acetamiprid resistance in melon aphid, Aphis gossypii

Cited by 61 publications

References 58 publications

RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly, Zeugodacus cucurbitae

RNAi-Mediated Knockdown of Imaginal Disc Growth Factors (IDGFs) Genes Causes Developmental Malformation and Mortality in Melon Fly, Zeugodacus cucurbitae

The glutathione S-transferase (PxGST2L) may contribute to the detoxification metabolism of chlorantraniliprole in Plutella xylostella(L.)

Sublethal Effects of Bifenazate on Biological Traits and Enzymatic properties in the Panonychus citri (Acari: Tetranychidae)

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