Functional analysis of metals distribution in organs of the beetle Chrysolina pardalina exposed to excess of nickel by Micro-PIXE

Przybyłowicz, W.J.; Przybylowicz, Jolanta Mesjasz; Migula, P.; Głowacka, E.; Nakonieczny, Mirosław; Augustyniak, Maria

doi:10.1016/s0168-583x(03)01029-2

Cited by 38 publications

(30 citation statements)

References 9 publications

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“…1). We suppose that presence of more Ni in this region was due to intensive transport through the filter chamber and its elimination with the honeydew straight to the hindgut without entering the midgut (Przybyłowicz et al 2003). In N. berkheyae such region with higher Ni concentration was also present (Fig.…”

Section: Discussionmentioning

confidence: 80%

“…Earlier studies of Crawford et al (1995) showed that dietary levels of cadmium or copper had little or no effect on the performance of the aphid Aphis fabae. Not all ingested metals are harmful, due to various mechanisms of internal sequestration or rapid transport from the foregut directly to the hindgut, leading to isolation of metals from the metabolic pathways (Przybyłowicz et al 2003;Vijver et al 2004). Elemental distribution can be obtained for the whole bodies or for isolated body organs using the micro-PIXE (particle induced X-ray emission).…”

Section: Introductionmentioning

confidence: 99%

“…Elemental distribution can be obtained for the whole bodies or for isolated body organs using the micro-PIXE (particle induced X-ray emission). This technique was earlier used to search for strategies of Ni tolerance in various insect species associated with B. coddii (Przybyłowicz et al 2003;2004). In the present work we checked whether transfer of Ni from the plant to sap-feeders might influence elemental distributions within insect's body.…”

Section: Introductionmentioning

confidence: 99%

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Micro-PIXE studies of elemental distribution in sap-feeding insects associated with Ni hyperaccumulator, Berkheya coddii

et al. 2007

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The aim of this study was to determine elemental composition of sap-feeding insects inhabiting various parts of the Ni hyperaccumulating plant Berkheya coddii Roessl., the endemic species of ultramafic outcrops in Mpumalanga, South Africa. Three species were examined: the aphid Protaphis pseudocardui (Aphididae), abundant on young leaves; the mealybug Orthesia sp. (Ortheziidae) colonizing underground parts of this plant, and the bug Norialsus berkheyae (Cixiidae) living on young shoots. Maps of Ni, K, Ca, Zn, and Fe for selected body areas of these species were generated using Dynamic Analysis method on the basis of particle-induced X-ray emission (micro-PIXE) and proton backscattering (BS) measurements. Atomic absorption spectrometry was used to determine Ni, Zn, Cu, Fe contents in the B. coddii organs, in some sapfeeding insect species including these mentioned above, and in the assassin bug hunting on Chrysolina pardalina, a monophagous beetle of B. coddii. Bioaccumulation factor for Ni in the examined species was below 0.05, and much higher for other metals (Zn ‡ 2; Fe £ 5). Ni distribution within body was species-dependent. It was the highest in the antennae of P. pseudocardui, in the head of Orthesia sp. and in the metathorax of N. berkheyae. Distribution patterns of other metals were different among examined species. Ca was recorded mainly in peripheral parts of the body in all species. Zn showed similar distribution to Ni. Fe distribution was similar to Ni only in the mealybugs. Uneven concentrations of metals within selected body regions indicated their relations with specific organs. Analysis of Ni transfer to higher trophic levels was done on the basis of two food nets: B. coddii-C. pardalina-Rhinocoris neavii and B. coddii-P. pseudocardui-Polyrhachis ant and led to the conclusion that the role of sap-feeding insects in Ni transfer was marginal.

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Section: Discussionmentioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micro-PIXE studies of elemental distribution in sap-feeding insects associated with Ni hyperaccumulator, Berkheya coddii

et al. 2007

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“…Cadmium can be easily determined by the PIXE technique, which allow us to correlate its quantity with toxicity of intracellularly accumulated metal ions. PIXE has been conventionally used to estimate metal contents in organic and inorganic materials (Kozai et al 2003;Przybylowicz et al 2003).…”

Section: Resultsmentioning

confidence: 99%

The function of Alr1p of Saccharomyces cerevisiae in cadmium detoxification: Insights from phylogenetic studies and particle-induced X-ray emission

et al. 2005

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Two genes in Saccharomyces cerevisiae, ALR1 and ALR2, encode transmembrane proteins involved in Mg2+ uptake. The present study investigates the phylogenetic relationship of Alr1p/Alr2p with bacterial CorA proteins and some proteins related to Mg2+ influx/efflux transport in mitochondrial and bacterial zinc transporters; including hydrophobic cluster analysis (HCA). The phylogenetic results indicate that the Alrp sequences of S. cerevisiae share a common carboxy-terminus with proteins related to zinc efflux transport. We also analyse the intracellular metal content by particle-induced X-ray emission (PIXE) after cell exposure to cadmium. The PIXE analysis of cadmium-exposed ALR mutants and wild-type yeast cells suggests that Alrp has a central role in cell survival in a cadmium-rich environment.

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“…These techniques include cryofixation of specimens to immobilize Ni in tissues after death, and elemental analysis using sensitive microscopic techniques (such as microscopic proton-induced X-ray emission, or Micro-PIXE). In a series of pioneering studies using this technique (Mesjasz- Przybylowicz et al, 2003;Przybylowicz et al, 2004;Augustyniak et al, 2008), five insect species (including the high-Ni insects Chrysolina pardalina and Stenoscepa sp.) that feed on the South African Ni hyperaccumulator Berkheya coddii have been analyzed.…”

Section: Where Is Ni In Insect Bodies?mentioning

confidence: 99%

High‐nickel insects and nickel hyperaccumulator plants: A review

Boyd

2009

Insect Science

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Functional analysis of metals distribution in organs of the beetle Chrysolina pardalina exposed to excess of nickel by Micro-PIXE

Cited by 38 publications

References 9 publications

Micro-PIXE studies of elemental distribution in sap-feeding insects associated with Ni hyperaccumulator, Berkheya coddii

Micro-PIXE studies of elemental distribution in sap-feeding insects associated with Ni hyperaccumulator, Berkheya coddii

The function of Alr1p of Saccharomyces cerevisiae in cadmium detoxification: Insights from phylogenetic studies and particle-induced X-ray emission

High‐nickel insects and nickel hyperaccumulator plants: A review

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