Functional analysis of serum microRNAs miR-21 and miR-106a in renal cell carcinoma

Tusong, Hamulati; Maolakuerban, Naibijiang; Guan, Jian; Rexiati, Mulati; Wang, Wenguang; Azhati, Baihetiya; Nuerrula, Yiliyaer; Wang, Yujie

doi:10.3233/cbm-160676

Cited by 55 publications

(53 citation statements)

References 20 publications

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“…Emerging evidence suggests that miRNAs are involved in tumor development and progression (19). In the present study, miR-136-5p was revealed to be downregulated in RCC tissues and cell lines compared with adjacent non-tumor tissues and cells in vitro.…”

Section: Discussionsupporting

confidence: 48%

Tumor suppressor microRNA‑136‑5p regulates the cellular function of renal cell carcinoma

et al. 2018

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Abstract. MicroRNAs (miRs) are involved in diverse physiological and developmental processes at the post-transcriptional level in cells. Previous studies have demonstrated that miR-136-5p is involved in certain types of cancer. However, the function of miR-136-5p in renal cell carcinoma (RCC) remains to be fully elucidated. In present study, miR-136-5p expression levels were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and MTT assays, CCK-8 assays, Transwell assays, wound healing assays and flow cytometry were performed to investigate the function of miR-136-5p in RCC. RT-qPCR revealed that the expression of miR-136 was significantly lower in RCC tissues and cells compared with adjacent non-tumor tissues and cells in vitro. miR-136-5pwas also demonstrated to be associated with RCC cell proliferation, viability, migration, invasion and apoptosis. miR-136-5p may therefore function as a tumor suppressor in RCC. Further studies are required to elucidate the molecular mechanisms and signaling pathways underlying these functions of miR-136-5p, to investigate the potential function of miR-136-5p as a biomarker for the early detection and prognosis of RCC, and its potential as a therapeutic target for the treatment of RCC.

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Section: Discussionsupporting

confidence: 48%

Tumor suppressor microRNA‑136‑5p regulates the cellular function of renal cell carcinoma

et al. 2018

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“…Transwell chamber inserts (BD Biosciences, Franklin Lakes, NJ, USA) with (for invasion) or without Matrigel (for migration) were used in the assay according to the manufacturer's protocol. Briefly, 200 µl serum-free medium containing ~1x10 4 transfected cells was plated in the upper compartment of the chamber. Complete medium as the source of chemo-attractants was added into the lower chamber.…”

Section: Micrornamentioning

confidence: 99%

“…Clear cell RCC is the most common subtype accounting for approximately 75% of all RCC cases (2). According to the American Joint Committee on Cancer staging of RCC (3), the 5-year survival rate of patients with stage I RCC is ~98%, while that of patients with stage III is ~5% (4). Thus, early detection and treatment is important.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‑191‑5p exerts a tumor suppressive role in renal cell carcinoma

et al. 2017

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Abstract. Renal cell carcinoma (RCC) is a common tumor of the urinary system. Previously, miR-191-5p has been reported to be associated with various types of cancer; however, its specific functions in RCC have not been investigated to date. In the present study, the expression of miR-191-5p in the 786-O and ACHN cell lines was detected in vitro by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results of RT-qPCR revealed that miR-191-5p was significantly downregulated in the two cell lines compared with the 293T cell line. miR-191-5p was also significantly downregulated in RCC tissue compared with paired normal tissue. In addition, the effects of miR-191-5p on cell proliferation, migration, invasion and apoptosis were examined by CCK-8, MTT, wound scratch, Transwell and flow cytometry assays. Downregulation of miR-191-5p was observed to promote cell proliferation, migration and invasion, as well as to repress the cell apoptosis of 786-O and ACHN cells. Therefore, the current study suggests that miR-191-5p functions as a tumor suppressor in RCC. Further studies are required to uncover the underlying signaling pathway of miR-191-5p and its potential role as a biomarker for early detection and prognosis prediction, and as a therapeutic target of RCC.

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“…The expression of miR-106 has been studied in renal cell carcinoma (RCC) [18], osteosarcoma [19], and gastric [20], lung [21], and colorectal [22] cancers. Moreover, the diagnostic and prognostic significance of miR-106 has also been explored in several cancer types.…”

Section: Introductionmentioning

confidence: 99%

Matrine induced G0/G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia (T-ALL) cells

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et al. 2017

Bosn J of Basic Med Sci

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Matrine, a natural product extracted from the root of Sophora flavescens, is a promising alternative drug in different types of cancer. Here, we aimed to investigate the therapeutic effects and underlying molecular mechanisms of matrine on human acute lymphoblastic leukemia (ALL) cell line, CCRF-CEM. Cell viability and IC50 values were determined by WST-1 cell cytotoxicity assay. Cell cycle distribution and apoptosis rates were analyzed by flow cytometry. Expression patterns of 44 selected miRNAs and 44 RNAs were analyzed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using the Applied Biosystems 7500 Fast Real-Time PCR System. Matrine inhibited cell viability and induced apoptosis of CCRF-CEM cells in a dose-dependent manner. Cell cycle analysis demonstrated that matrine-treated CCRF-CEM cells significantly accumulated in the G0/G1 phase compared with the untreated control cells. hsa-miR-376b-3p (-37.09 fold, p = 0.008) and hsa-miR-106b-3p (-16.67 fold, p = 0.028) expressions were decreased, whereas IL6 (95.47 fold, p = 0.000011) and CDKN1A (140.03 fold, p = 0.000159) expressions were increased after matrine treatment. Our results suggest that the downregulation of hsa-miR-106b-3p leads to the upregulation of target p21 gene, CDKN1A, and plays a critical role in the cell cycle progression by arresting matrine-treated cells in the G0/G1 phase.

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Functional analysis of serum microRNAs miR-21 and miR-106a in renal cell carcinoma

Abstract: We conclude that serum miR-21 and miR106a are expected to be molecular markers for RCC.

Cited by 55 publications

References 20 publications

Tumor suppressor microRNA‑136‑5p regulates the cellular function of renal cell carcinoma

Tumor suppressor microRNA‑136‑5p regulates the cellular function of renal cell carcinoma

MicroRNA‑191‑5p exerts a tumor suppressive role in renal cell carcinoma

Matrine induced G0/G1 arrest and apoptosis in human acute T-cell lymphoblastic leukemia (T-ALL) cells

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