Functional analysis target of rapamycin (TOR) on the Penaeus vannamei in response to acute low temperature stress

Liang, QingJian; Ou, MuFei; Li, Zhonghua; Ren, Yinghao; Wei, Wei; Qiao, Xue-Li; Hu, Rui; Wu, XuJian; Liu, Yuan; Wang, Weina

doi:10.1016/j.fsi.2019.11.070

Cited by 22 publications

(7 citation statements)

References 41 publications

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“…Previous studies have shown that loss of circulating hemocytes or hemocyte DNA damage would endanger survival (Li et al, 2013;Liang et al, 2019a). Thus, this possibility was observed, and results showed that overexpression of pva-miR-151 significantly elevated THC, while silencing pva-miR-151 significantly reduced the THC in low-temperature challenge (Figures 5D,E).…”

Section: Alters Of Pva-mir-151 Impact Low-temperature Tolerance Of Shrimpmentioning

confidence: 68%

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

Liang

Dong

et al. 2021

Front. Cell Dev. Biol.

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MicroRNAs (miRNAs) play key roles in many physiologic and pathologic processes, including autophagy. Autophagy is cellular in an emergency response mechanism of environment stress, but their complex molecular regulatory mechanism under low-temperature stress is largely unknown in shrimp, especially miRNA-mediated regulation of autophagy in low-temperature tolerance. In this article, a shrimp PvTOR and miRNA pva-miR-151 cooperation in response to low-temperature stress has been reported. Pva-miR-151 showed expression patterns opposite to target PvTOR under low-temperature stress. The pva-miR-151 targets the 3′-UTR region of PvTOR, regulate the formation of autophagosome, which contribute to the degradation and recycling of damaged organelles. In addition, the low-temperature tolerance was correlated positively with autophagy in shrimp. Silenced pva-miR-151 increased sensitivity to low-temperature stress, whereas overexpression pva-miR-151 decreased the expression of PvTOR and p-TOR and increased tolerance to low-temperature stress by improving the formation of autophagosome and total hemocyte count. In addition, the TOR activator 3BDO can partially rescue autophagy induced by overexpression of pva-miR-151; these results indicate that miR-151 was necessary for the low-temperature tolerance in shrimp. Taken together, we provide a novel strategy and mechanism for shrimp breeding to improve shrimp low-temperature tolerance.

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Section: Alters Of Pva-mir-151 Impact Low-temperature Tolerance Of Shrimpmentioning

confidence: 68%

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

Liang

Dong

et al. 2021

Front. Cell Dev. Biol.

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“…Autophagy is a relatively conservative cell degradation process in evolution, through which cells recycle ineffectual cellular components (such as damaged DNA, damaged organelles, intracellular pathogens, and misfolded proteins) to maintain homeostasis [56]. The autophagy-lysosome system could clean the oxidized, damaged, and misfolded proteins induced by environmental stress in the cell [58,59]. A recent study reported that low temperature-induced autophagy is a strategy to adapt to and cope with stress in Penaeus vannamei [59].…”

Section: Discussionmentioning

confidence: 99%

Application of Transcriptome Analysis to Understand the Adverse Effects of Hypotonic Stress on Different Development Stages in the Giant Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

et al. 2022

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Salinity is one of the important environmental factors affecting survival and growth of aquatic animals. However, the impact of low-salinity stress on M. rosenbergii post-larvae at different development stages remains elusive. Therefore, the aim of this study was to explore the underlying mechanisms of hypotonic stress at different development stages of M. rosenbergii post-larvae through transcriptome analysis and antioxidant parameters detection. The salinity of the control group was 15 psu (S15) and the hypotonic stress group was 6 psu (S6). Samples were collected at 7 days-post-hatch (dph), 14 dph and 21 dph larvae. The results showed that hypotonic stress caused oxidative damage in post-larvae evidenced by decreased glutathione peroxidase (GSH-Px); superoxide dismutase (SOD); anti-superoxide anion free radical (ASAFR); and increased malondialdehyde (MDA); nitric oxide (NO); and inducible nitric oxide synthase (iNOS) levels. Transcriptome analysis showed that there were 1428, 1187, 132 DEGs including 301, 366, 4 up-regulated genes and 1127, 821, 128 down-regulated genes at 7 dph, 14 dph and 21 dph larvae under hypotonic stress, respectively. Furthermore, GO and KEGG enrichment indicated that hypotonic stress led to dysregulation of immune signals including lysosome and autophagy in the 7 dph larvae. The autophagy-related genes including beclin 1-associated autophagy-related key regulator (Barkor); ubiquitin-like modifier-activating enzyme ATG7 (ATG7); Beclin; autophagy-related protein 13 (ATG13); nuclear receptor-binding factor 2 (Nrbf2); ubiquitin-like-conjugating enzyme ATG3 (ATG3); vacuole membrane protein 1 (VMP1); and autophagy-related protein 2 (ATG2) decreased at 7 dph, and 14 dph larvae, and then increased at 21 dph larvae under hypotonic stress. In the 14 dph and 21 dph larvae, the renin-angiotensin system was activated. In conclusion, our data indicated that hypotonic stress reduced the antioxidant capacity and impaired the immune system in post-larvae, but as development progresses, the adaptability of post-larvae to hypotonic stress gradually increased, and might reach a new homeostasis through the RAS signaling pathway.

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“…The tissue lysate was mixed with 5× SDS-PAGE buffer (Beyotime) and boiled for 10 min. Then, 30 μg of each sample was subjected to 10% SDS-PAGE and Western blotting, as previously reported [ 24 ]. The primary antibody diluted 1:1000 and secondary antibody diluted 1:2000 in 0.5% BSA-TBST.…”

Section: Methodsmentioning

confidence: 99%

“…The tissue was lysed in RIPA buffer (Beyotime, China), containing 1 mm PMSF for 30 min at 4 • C, and centrifuged at 12,000× g for 15 min at 4 • C. The tissue lysate was mixed with 5× SDS-PAGE buffer (Beyotime) and boiled for 10 min. Then, 30 µg of each sample was subjected to 10% SDS-PAGE and Western blotting, as previously reported [24]. The primary antibody diluted 1:1000 and secondary antibody diluted 1:2000 in 0.5% BSA-TBST.…”

Section: Western Blottingmentioning

confidence: 99%

A Novel Kelch-Like-1 Is Involved in Antioxidant Response by Regulating Antioxidant Enzyme System in Penaeus vannamei

Qiao

Liang

Liu

et al. 2020

Genes

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Heavy metals are typical cumulative pollutants that can enter and poison the human body through the food chain. However, the molecular mechanism of heavy metal-induced oxidative stress is unclear. In this study, we characterize PvKelch-like-1 from P. vannamei and explore its antioxidant roles in immune regulation of crustaceans. PvKelch-like-1 full length contains 2107 nucleotides, consists of a 5′ untranslated region (UTR) of 79 bp, a 3′ UTR of 180 bp, and a ORF of 1848 encoded 615 amino acids, which contain a BTB, BACK and Kelch motif, putative molecular mass and isoelectric point were 69 KDa and 6.54. PvKelch-like-1 mRNA was ubiquitously expressed in all detected tissue of P. vannamei, and mRNA expression levels were significantly up-regulated from 6 to 24 h after cadmium stress and reached the highest level (3.2-fold) at 12 h in the hepatopancreas. Subcellular localization analysis revealed that PvKelch-like-1 was localized in the nucleus. Silencing PvKelch-like-1 significantly increased reactive oxygen species (ROS) (1.61-fold) production and DNA damage (1.32-fold) in the shrimp hemolymph, and significantly decreased total hemocyte counts (THC) (0.64-fold) at 6 h in hemolymph. Additionally, the antioxidant genes PvCAT (0.43-fold), PvMnSOD (0.72-fold), PvGST (0.31-fold) and PvGPx (0.59-fold) at 6 h were decreased significantly in PvKelch-like-1 silenced shrimp after cadmium stress. Overexpression of PvKelch-like-1 has the opposite results in enzyme activity. The SOD (2.44-fold) and CAT (2.19-fold) activities were significantly increased after overexpressing PvKelch-like-1. These results suggest that PvKelch-like-1 plays a vital role in shrimp innate immune defense by positively regulating the expression of antioxidant enzyme genes to respond to cadmium stress.

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Functional analysis target of rapamycin (TOR) on the Penaeus vannamei in response to acute low temperature stress

Cited by 22 publications

References 41 publications

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

miR-151 Affects Low-Temperature Tolerance of Penaeus vannamei by Modulating Autophagy Under Low-Temperature Stress

Application of Transcriptome Analysis to Understand the Adverse Effects of Hypotonic Stress on Different Development Stages in the Giant Freshwater Prawn Macrobrachium rosenbergii Post-Larvae

A Novel Kelch-Like-1 Is Involved in Antioxidant Response by Regulating Antioxidant Enzyme System in Penaeus vannamei

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