Functional and transcriptomic analyses of the NF-Y family provide insights into the defense mechanisms of honeybees under adverse circumstances

Li, Guilin; Zhao, Hang; Guo, Hongbin; Wang, Ying; Cui, Xuepei; Xu, Baohua; Guo, Xingqi

doi:10.1007/s00018-019-03447-0

Cited by 9 publications

(7 citation statements)

References 62 publications

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“…superoxide dismutase, glutathione S -transferase, catalase) and genes involved with antioxidant defence mechanisms (e.g. thioredoxin) ( Choi et al., 2006 ; Orčić et al., 2017 ; Li et al., 2019 ; Li et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, floral nectar is generally low in antioxidants ( Baker, 1977 ), which may constrain the capacity of B. impatiens to combat oxidative damage and/or select for enhanced capacities for endogenous antioxidant production, such as the upregulation of various genes (e.g. thioredoxin) linked to antioxidant defence ( Choi et al., 2006 ; Li et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Heat exposure limits pentose phosphate pathway activity in bumblebees

Rossi,

Elbassiouny,

Jamison

et al. 2024

Conservation Physiology

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Bumblebee populations across the globe are experiencing substantial declines due to climate change, with major consequences for pollination services in both natural and agricultural settings. Using an economically important species, Bombus impatiens, we explored the physiological mechanisms that may cause susceptibility to extreme heat events. We tested the hypothesis that heat exposure limits the activity of the pentose phosphate pathway (PPP)—a parallel pathway to glycolysis that can use nectar sugar to generate antioxidant potential and combat oxidative stress. Using isotopically labelled glucose, we tracked PPP activity in B. impatiens at rest, during exercise and during a post-exercise recovery period under two different temperature regimes (22°C and 32°C). We found that the PPP is routinely used by B. impatiens at moderate temperatures, but that its activity is markedly reduced when ATP demands are high, such as during periods of exercise and heat exposure. We also exposed B. impatiens to either 22°C or 32°C for 5 hours and assessed levels of oxidative damage (lipid peroxidation, protein carbonyls) and antioxidant potential [reduced (GSH) and oxidized (GSSG) glutathione concentrations]. Interestingly, bees exhibited little oxidative damage after the thermal exposure, but we found a lower GSH:GSSG ratio in 32°C-exposed bees, reflecting lower antioxidant potential. Overall, our study demonstrates that acute heat stress severely limits PPP activity and may constrain antioxidant potential in B. impatiens. The repeated attenuation of this pathway in a warming climate may have more severe physiological consequences for this species, with potential implications for pollination services across North America.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Heat exposure limits pentose phosphate pathway activity in bumblebees

Rossi,

Elbassiouny,

Jamison

et al. 2024

Conservation Physiology

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“…The expression level of AccJNK was significantly upregulated after treatment, indicating that pesticides stimulated the expression of AccJNK , causing harm to Acc . Pesticide exposure induces oxidative stress by increasing ROS production (Li et al ., 2020). Abamectin is a commonly used insecticide which induces cytotoxicity through the ROS‐mediated JNK pathway, causing oxidative stress in mammals, and resulting in DNA double‐strand breaks and cell apoptosis (Feng et al ., 2020).…”

Section: Discussionmentioning

confidence: 99%

Role of c‐Jun NH₂‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana

et al. 2022

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The mitogen-activated protein kinase (MAPK) cascade pathway plays an important role in regulating stress responses. The function of the c-Jun NH 2 -terminal kinase (JNK), a component of the MAPK cascade pathway, in Apis cerana cerana (Acc) remains unclear. Here, JNK was isolated and identified from Acc. Bioinformatics analyses revealed there is a typical serine/threonine protein kinase catalytic domain in the AccJNK protein. An expression profile analysis showed that AccJNK was significantly induced by pesticide treatments. To further explore the functional mechanisms of AccJNK, a yeast 2-hybrid screen was performed, activator protein-1 (AP-1) was screened as the interaction partner of AccJNK, and the interaction relationship was further verified by pull-down assay. Quantitative real-time polymerase chain reaction showed the expression pattern of AccAP-1 was similar to that of AccJNK. After a knockdown of AccJNK or AccAP-1 by RNA interference, the survival rate of Acc after pesticide treatments increased. Additionally, the expression levels of antioxidant-related genes and the activities of antioxidant enzymes increased, suggesting that the knockdown of AccJNK or AccAP-1 increased the antioxidant capacity of bees. Our study revealed that the JNK-mediated MAPK pathway responds to pesticide stress by altering the antioxidant capacity of Acc.

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“…Previous studies conducted in our laboratory revealed that AcZFP41 was significantly induced by RNA‐Seq analysis under heat stress treatment (Li et al, 2020); so, we were fortunate to investigate it. In our study, AcZFP41 was isolated from A. cerana , and its function in the responses to oxidative stress was demonstrated.…”

Section: Introductionmentioning

confidence: 94%

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

Guo

Wang

et al. 2021

Arch Insect Biochem Physiol

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Zinc finger proteins (ZFPs) are a class of transcription factors that contain zinc finger domains and play important roles in growth, aging, and responses to abiotic and biotic stresses. These proteins activate or inhibit gene transcription by binding to single‐stranded DNA or RNA and through RNA/DNA bidirectional binding and protein–protein interactions. However, few studies have focused on the oxidation resistance functions of ZFPs in insects, particularly Apis cerana. In the current study, we identified a ZFP41 gene from A. cerana, AcZFP41, and verified its function in oxidative stress responses. Real‐time quantitative polymerase chain reaction showed that the transcription level of AcZFP41 was upregulated to different degrees during exposure to oxidative stress, including that induced by extreme temperature, UV radiation, or pesticides. In addition, the silencing of AcZFP41 led to changes in the expression patterns of some known antioxidant genes. Moreover, the activities of the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and glutathione S‐transferase (GST) in AcZFP41‐silenced honeybees were higher than those in a control group. In summary, the data indicate that AcZFP41 is involved in the oxidative stress response. The results provide a theoretical basis for further studies of zinc finger proteins and improve our understanding of the antioxidant mechanisms of honeybees.

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Functional and transcriptomic analyses of the NF-Y family provide insights into the defense mechanisms of honeybees under adverse circumstances

Cited by 9 publications

References 62 publications

Heat exposure limits pentose phosphate pathway activity in bumblebees

Heat exposure limits pentose phosphate pathway activity in bumblebees

Role of c‐Jun NH₂‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

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Functional and transcriptomic analyses of the NF-Y family provide insights into the defense mechanisms of honeybees under adverse circumstances

Cited by 9 publications

References 62 publications

Heat exposure limits pentose phosphate pathway activity in bumblebees

Heat exposure limits pentose phosphate pathway activity in bumblebees

Role of c‐Jun NH2‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana

Identification of an Apis cerana zinc finger protein 41 gene and its involvement in the oxidative stress response

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Role of c‐Jun NH₂‐terminal kinase‐mediated mitogen‐activated protein kinase pathway in response to pesticides in Apis cerana cerana