Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from <i>Taxus</i> Rhizosphere

Hao, Da‐Cheng; Song, Si Meng; Cheng, Yanwei; Qin, Zhi Qiang; Ge, Guang Bo; An, Bai Lin; Xiao, Pei

doi:10.21307/pjm-2018-050

Cited by 7 publications

(3 citation statements)

References 43 publications

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“…There were reports of peroxidase/polyhenol oxidase activities by some P. chrysogenum isolates, while no peroxidase/polyhenol oxidase activities were reported for others [50,51]. To the authors' best knowledge, there have been no reports on these activities from P. antarcticum.…”

Section: Xylanase Induction By Wb During Lsf and The Qualitative Asse...mentioning

confidence: 84%

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Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Dowd,

Tuohy

2023

Fermentation

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Fungi are increasingly recognised as being able to inhabit extreme environments. The deep sea is considered an extreme environment because of its low temperatures, high hydrostatic and lithostatic pressures, 3.5% salinity, and low oxygen, nutrient and light availability. Fungi inhabiting the deep sea may have evolved to produce proteins that allow them to survive these conditions. Investigation and characterisation of fungal lignocellulolytic enzymes from extreme environments like the deep sea is needed, as they may have unusual adaptations that would be useful in industry. This work, therefore, aimed to profile in detail the lignocellulolytic capabilities of fungi isolated from deep-sea sediments in the Atlantic Ocean, and a comparative lignocellulolytic terrestrial isolate. The isolates were strains of Emericellopsis maritima, Penicillium chrysogenum, P. antarcticum and Talaromyces stollii. Lignocellulolytic enzyme induction was achieved using liquid-state fermentation (LSF) with wheat bran as the main carbon source, while enzyme characteristics were evaluated using biochemical assays and gel-based proteomics. This study revealed that the isolates were halotolerant, produced xylanase over wide pH and temperature ranges, and produced a variety of glycoside hydrolase and feruloyl esterase activities. The T. stollii secretome demonstrated remarkable levels of exo-glycoside hydrolase activity, with xylanase activity optimum between pH 1.5–6.0 and temperatures between 1–60 °C, making this isolate an ideal candidate for biotechnological applications. This study is the first to quantitatively characterise xylanase activities and exo-glycoside hydrolase activities secreted by E. maritima, P. antarcticum and a marine T. stollii strain. This study is also the first to quantitatively characterise xylanase activities by a marine strain of P. chrysogenum during LSF.

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Section: Xylanase Induction By Wb During Lsf and The Qualitative Asse...mentioning

confidence: 84%

“…To the authors' best knowledge, there have been no reports on these activities from P. antarcticum. T. stollii was previously reported to produce laccase activity [51], while no other polyphenol oxidase/peroxidase activities have so far been recorded for this species.…”

Section: Xylanase Induction By Wb During Lsf and The Qualitative Asse...mentioning

confidence: 85%

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Dowd,

Tuohy

2023

Fermentation

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“…The development of advanced sequencing technology allows the investigation of molecular mechanisms involved in the detoxi cation and adaptation of microbial strains to environmental pollutant exposure [34][35][36] . For example, transcriptomic analysis using differentially expressed genes (DEGs) has been used to examine the gene responses to various environmental stimuli, particularly for the elucidation of genes involved in degradation [37][38][39] . Transcriptome sequencing can provide strong support for identifying genes involved in pollutant degradation by microorganisms 40 .…”

Section: Introductionmentioning

confidence: 99%

Molecular Response Mechanism of Pseudomonas Nicosulfuronedens LAM1902 to A Typical Sulfonylurea Herbicide Nicosulfuron

Yao

et al. 2022

Preprint

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The overuse of the herbicide nicosulfuron (NS) has become a global environmental concern. As a potential bioremediation technology, the microbial degradation of NS shows much promise; however, the detailed mechanisms of microbial taxa responding to NS exposure require further study. An isolated soil-borne bacteria Pseudomonas nicosulfuronedens LAM1902 displaying NS degradabilities was used to characterize the molecular responses to NS exposure, and LAM1902 can degrade exceed 95% of 50 mg/L nicosulfuron under the optimal conditions. Using transcriptomic sequencing, RNA-Seq results indicated that 1102 differentially expressed genes (DEGs) were up-regulated and 702 down-regulated in response to NS. Among them, “ABC transporters,” “sulfur metabolism,” and “ribosome” gene pathways were significantly enriched (p ≤ 0.05). Several gene pathways involved in the glycolysis and pentose phosphate pathways, a two-component regulation system, as well as in bacterial chemotaxis metabolisms were up-regulated under NS exposure. Surprisingly, NS exposure showed positive effects on the production of oxalic acid that is synthesized by genes encoding glycolate oxidase through the glyoxylate cycle pathway. The results suggest that P. nicosulfuronedens LAM1902 adopt acid metabolites production strategies in response to NS, with concomitant NS degradation. Meanwhile, strain LAM1902 likely grows and survives amid NS stress by increased energy production. The present studies provide a glimpse at the molecular response of microorganisms to sulfonylurea pesticide toxicity and a potential framework for future mechanistic studies.

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Mining pharmacotherapy utility from chemodiversity/biodiversity of Taxaceae- and Cephalotaxaceae-associated microbes: Molecular mechanisms and functions

Hao

2021

Taxaceae and Cephalotaxaceae

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Functional and Transcriptomic Characterization of a Dye-decolorizing Fungus from Taxus Rhizosphere

Cited by 7 publications

References 43 publications

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Induction and Characterisation of Lignocellulolytic Activities from Novel Deep-Sea Fungal Secretomes

Molecular Response Mechanism of Pseudomonas Nicosulfuronedens LAM1902 to A Typical Sulfonylurea Herbicide Nicosulfuron

Mining pharmacotherapy utility from chemodiversity/biodiversity of Taxaceae- and Cephalotaxaceae-associated microbes: Molecular mechanisms and functions

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